ThermoFisher Scientific Vanquish VH-D10, Operating Manual

Page: 136 / 196

7 • Maintenance and Service
Page 136 Vanquish Diode Array Detectors (VH-D10) Operating Manual
Determining the required pump flow
NOTICE Flow cells are highly sensitive to high pressures. Even if the
pressure exceeds the upper limit for a very short time only, the flow cell
may be permanently damaged. Do not connect the flow cell to the pump
for this pump flow determination procedure.
1. Connect the inlet line of the back-flush capillary to the outlet of the
pump.
2. Route the overpressure line of the back-flush capillary to waste.
3. Disconnect the Viper connector from the back-flush capillary and
connect the back-flush plug to the T piece.
Figure 32: Setup for determining the pump flow
No. Description
1 To the outlet of the pump
2 Plug
3 Waste container
4. Set the upper pressure limit of the pump to 60% of the specified
pressure limit of the flow cell plus 0.5 MPa.
5. Turn on the pump flow at a flow rate of 0.1 mL/min with the back-
flushing agent.
6. Slowly increase the pump flow until the pump pressure reaches 60%
of the specified pressure limit of the flow cell and write down the
determined value. If the pump pressure does not reach the 60%, set
the maximum pump flow.