5
•
Installation
Fluorescence Detectors (VC-D50, VC-D51, VF-D50, VF-D51)
Operating Manual
Page 77
5.7
Determining the Pressure inside the Flow Cell
All modules, capillaries and waste lines downstream of the flow cell
contribute to the pressure inside the flow cell. If you attach additional
modules such as detectors, fraction collectors or a mass spectrometer in
the flow path after the flow cell, make sure that the pressure within the
flow cell does not exceed its pressure specification.
When
•
If you install additional modules and capillaries in the flow path after
the flow cell:
¨
Before installing the mentioned additional modules
¨
After having installed the mentioned additional modules
•
Before you use a new method
•
After you have replaced capillaries in the flow path after the flow
cell
•
Depending on the application, for example, if you use high salt
concentrations, this procedure may help you troubleshooting.
Parts required
•
Union connector (for example, Viper union from the system ship kit)
•
Capillaries and waste line from the detector ship kit
•
One of the following solvent mixtures of high viscosity:
Gradient usage
Solvent Mixture
If using gradients
Solvent to be used in your application. Use the mixing ratio
with the highest viscosity.
If not using
gradients
•
60% water and 40% methanol
–or–
•
70% water and 30% acetonitrile
General Outline of the Procedure
1. Measure the backpressure of the waste line (see
Backpressure of the Waste Line (
Flow path: Pump – waste line
2. Measure the backpressure of the Vanquish system including the
column, detector inlet capillary and waste line (without installed
flow cell) (see
Measuring the Vanquish System Backpressure
).
Flow path: Pump – autosampler – column – waste line
Summary of Contents for Vanquish VC-D50
Page 4: ...Contacting Us Page 4 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 10: ...Contents Page 10 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 30: ...2 Safety Page 30 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 42: ...3 Device Overview Page 42 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 88: ...5 Installation Page 88 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 122: ...6 Operation Page 122 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 170: ...9 Specifications Page 170 Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual...
Page 187: ...Index Fluorescence Detectors VC D50 VC D51 VF D50 VF D51 Operating Manual Page 187...