ThermoFisher Scientific Vanquish VC-D50, Operating Manual

Page: 104 / 188

6 • Operation
Page 104 Fluorescence Detectors (VC-D50, VC-D51, VF-D50, VF-D51)
Operating Manual
6.8 Important Operating Parameters
The commands and parameters described in the table should be
considered for simple routine operation of the device. You can usually
access these parameters from the Chromeleon user interface.
If a parameter listed below is not available in the Chromeleon software,
consider updating the firmware and Chromeleon version.
TIP The Instrument Method Wizard provides different parameter view
modes. Depending on the parameters to be set, you can select the
desired view mode (for example,
Easy or Advanced ).
For more information, refer to Chromeleon Help and documents .
Parameter Description
Emission signal
parameters The Emission signal channels ( Emission_x ) are the signal
channels for recording an excitation/emission wavelength
pair. Signal parameters are settable for each signal channel
separately. For guidelines on selecting the signal
parameters, see
Optimizing the Performance of the Device
( } page 106) .
Excitation wavelength Sets the excitation wavelength in nm. Select the excitation
wavelength at the absorption maximum of the sample
component to be analyzed.
Emission wavelength Sets the emission wavelength in nm. The emission
wavelength must be at least 20 nm above the excitation
wavelength.
ZeroOrder : The grating of the emission monochromator is
set to the position of zero order. For details, see Zero Order
Mode ( } page 100) .
Sensitivity Sets the detector sensitivity, see Sensitivity (Detector
Sensitivity) ( } page 108) .
Data collection rate Sets the number of data points per second (Hz) that the
Chromeleon software collects from the detector and stores
as raw data.
For further information, see Data Collection Rate and
Response Time (Single-Channel Mode) (
}
page 113) .
Lamp mode Sets the mode of operation for the xenon flash lamp. A
higher frequency of flashes improves the baseline noise and
thus improves sensitivity. A lower flash frequency extends
the lifetime of the lamp.
For details, see Lamp Mode ( } page 115) .
Effective lamp age Provides information about the lamp operating hours to
help you in estimating the remaining lamp lifetime. For
further information, see
Monitoring the Lamp Age
( } page 131) .