Troubleshoot verification failure
Observation
Possible cause
Recommended action
Verification failed
The plate was improperly
prepared.
Ensure the following:
• The correct plate was used for the
verification performed.
• The plate was properly thawed.
• The plate was properly centrifuged.
• The plate was properly sealed.
Open the data file for verification in the desktop
software or the Connect platform to view the
flags and the troubleshooting details for the
failed wells.
The plate is damaged or
contaminated.
Check for damage, improper plate seal, or
contamination.
Order a replacement plate. Use Smart Help if
the replacement plate fails (see “Request
technical support with Smart Help” on
page 159).
High fluorescence signal
The reaction volume is not
correct.
Ensure that reaction volumes in the plate are
correct and match the volume that is entered in
the Method tab.
Signals that exceed the limit of
normal fluorescence can
indicate fluorescent
contaminants on the plate or on
the sample block.
Examine the bottom of the reaction plate. If
there is contamination, prepare a new plate and
run the experiment again.
Identify the location of contamination on the
plate or sample block.
1. Obtain or prepare a background plate (see
“Create a background plate (optional)” on
page 90).
2. Follow the procedures to identify
contamination (see “Identify contamination”
on page 90).
Verification failed but plate is
undamaged
The incorrect plate was used for
verification.
Use the correct RNase P plate for verification.
Chapter 7
Calibrate and verify instrument performance
Perform verification using RNase P plates
7
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QuantStudio
™
6 Pro and 7 Pro Real-Time PCR Systems User Guide
