Method elements
2
3
1
4
5
9
8
7
6
1
Method
tab
2
Heated cover temperature
3
Reaction volume
4
Stage of thermal protocol
5
Step within a stage
6
Number of cycles for the stage
7
Temperature for the step
8
Hold time for the step
9
Data collection point
Edit the method
1.
In the
Method
tab, tap
Edit
.
2.
Tap a field, then enter the changes.
• Cover temperature
• Volume
• Temperature for any steps
• Time for any steps
• Number of PCR cycles
3.
Tap
Save
.
4.
Enter a name for the plate file, then tap
Done
.
5.
In the
Save Template
screen, select a storage location for the plate file, then tap
Done
.
A confirmation is displayed.
6.
Tap
Close
.
Chapter 4
Edit a plate before starting a run
Options to edit the method of a plate file
4
QuantStudio
™
6 Pro and 7 Pro Real-Time PCR Systems User Guide
55
