Thermo NanoDrop One, User Manual, Page: 195 / 312

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Measure a Micro-Volume Sample
Thermo Scientific NanoDrop One User Guide 195
Recommended sample volumes
Pipetting Samples
• Use recommended sample volumes to
ensure proper liquid column
formation.
• Use calibrated precision pipettor
(0–2 μL volume range) with
well-fitting, low-retention precision
tips to apply sample material to
instrument for measurement.
If using low accuracy (0-10 μL)
pipettor, use 2 μL sample volumes.
• Use new tip for each blank and sample
aliquot.
• Use new aliquot of sample for each
measurement.
• If solvents are used, make sure they are
compatible with the pedestals. (see
“Compatible Solvents” in
Hazardous
Materials ).
Application Sample Volume
Nucleic acid (aqueous solution) 1 μL
a
a
Use 2 μL for samples that contain materials that may reduce surface tension such as a surfactant.
Purified protein 2 μL
Other protein applications such as Bradford or BCA 2 μL
Microbial cell suspensions 2 μL