Thermo 6464K Instruction Manual Download Page 5

OM6464K Rev. 4    11 January 2004

Page 5 of 24

1.  Introduction

1.1 Product Description

The IEC Centra CL5 units are high-speed, multi purpose 
centrifuges used in medical, industrial, and scientific 

applications. They are availabe in two models:

• Ventilated (CL5)

• Refrigerated (CL5R)

Both models feature maintenance free brushless motors. 
Both models also accommodate swinging bucket or fixed 
angle rotors.  They can process a variety of tubes, bottles, 

and microsample tubes.  Swinging bucket styles include a 
4 x 250 mL or microplate carrier rotor.  The microplate 
rotor can process both standard and deepwell microplates.  

Fixed angle styles include the following rotor types:

• 6  x  85  mL

• 8  x  28  mL
• Microtube   

Operation Modes

The IEC Centra CL5 Series offers two operation modes: 

Manual Mode

: to enter temperature (IEC Centra CL5R 

only), speed/force, and time values for individual runs

Program Mode

: to define and save a maximum of 99 

specific sets of run parameters, to recall and reuse

Timing Modes

Three timing modes are available on each unit: 

• Automatic timed run
• Short spin (momentary)

• Hold (continuous mode).  

Other Features

Acceleration and brake rates may be controlled to optimize 
runs; rapid for fast separations or slow for delicate 
samples.  Repeat runs, with the same speed and time 

settings, may be achieved at the touch of a key.
A fail-safe cover interlock insures that the cover is closed 

before a run can begin, and keeps the cover closed until the 
rotor has reached a safe low speed (below 100 rpm), even 
in the event of a power failure.  
The rugged steel cabinet and rigid construction provide 
quiet operation and long-term reliability.

1.2  About This Manual

Contents

This manual contains information to install, operate, and 

maintain IEC Centra CL5 centrifuges. Differences between 
the refrigerated and ventilated models are noted in the text. 
This manual also contains speed and force, derating, 

chemical resistance, and decontamination tables.

Audience/Purpose

This manual is written for centrifuge operators.  In addition 
to operation information, it contains a few basic 
troubleshooting techniques, and a chapter on maintenance. 

This manual is not a guide for servicing centrifuge units.

Warnings, Cautions, and Notes

WARNING

WARNING

!

A WARNING advises against certain actions or 
situations that could result in personal injury.

CAUTION

!

A CAUTION advises against actions or situations that 
could damage equipment, produce inaccurate data, or 

invalidate a procedure.

NOTE 

provides useful information regarding an operation, 

function, or procedure.

2. Installation

2.1 Receive the Unit

All units are shipped in protective packaging. 
1. Follow the unpacking instructions on the carton. 
2. Inspect the unit upon receipt and immediately file any 

damage claims with the shipper/carrier. 

3. Complete and return the postage-paid warranty card.

2.2 Prepare the Installation Site

The unit normally resides on a bench top.
1. OPTIONAL: 

The IEC Centra CL5  (ventilated model) can be placed in 
a cold room (no colder than 4°C), for processing 

temperature-sensitive samples.  

CAUTION

!

When you remove the centrifuge from a cold 

environment, do not operate for a minimum of two 
hours to allow condensation to evaporate prior to 
use. 
NOTE

: When used in a cold room environment, some 

bearing noise may become evident. The bearing 
lubricant thickens at low temperatures. As the 

centrifuge speeds up, it is thinned and distributed more 
evenly. Once this occurs, any noise should subside.

2. Place the centrifuge on a clean, dry surface, to make 

certain that the suction feet at the bottom grip the 

surface firmly. Keep the area beneath the unit free of 
debris and loose materials.

CAUTION

!

The resting surface must be level, to ensure quiet, 
vibration-free operation.  A rigid and stable location 
is important.  An improperly loaded centrifuge may 

vibrate or move

3. Locate the centrifuge to allow a clearance of 8 cm 

(3 inches) on all sides of the unit, to ensure proper 

ventilation. 

Dimensions:

IEC Centra CL5R

IEC Centra CL5

Sample Loading Height

13.7“ (34.8 cm)

Cover Closed Height

15.5” (39.4 cm)

Cover Open Height

38.5” (97.8 cm)

Width

28” (71.1 cm)

20.3”

 

(51.4 cm)

Height

23.4” (59.4 cm)

Summary of Contents for 6464K

Page 1: ...EC Centra CL5 and CL5R Centrifuges OM6464K Rev 4 11 January 2004 CL5 VENTILATED Cat No 6464K 120 VAC 60Hz Cat No 6465K 220 240 VAC 50 60Hz CL5R REFRIGERATED Cat No 6466K 120 VAC 60Hz Cat No 6467K 220 240 VAC 50 60Hz Instruction Manual ...

Page 2: ......

Page 3: ... 1 Rotor and Accessories 6 3 2 Starting and Stopping a Run 7 3 3 Stored Programs 8 3 4 Rotor Recognition System 8 3 5 Refrigeration IEC Centra CL5R only 8 3 6 Front Panel 9 3 7 Diagnostic Messages and Error Codes 10 4 Applications 11 4 1 Introduction 11 4 2 Speed and Force Tables 12 4 3 Derating Tables for Dense Samples 14 4 4 Chemical Resistance Table 15 4 5 Decontamination Table 16 4 6 RCF Nomog...

Page 4: ...OM6464K Rev 4 11 January 2004 Page 4 of 24 ...

Page 5: ...nual is written for centrifuge operators In addition to operation information it contains a few basic troubleshooting techniques and a chapter on maintenance This manual is not a guide for servicing centrifuge units Warnings Cautions and Notes WARNING WARNING A WARNING advises against certain actions or situations that could result in personal injury CAUTION A CAUTION advises against actions or si...

Page 6: ...its new location ensure that the suction cups adhere correctly to the work surface 3 Operation 3 1 Rotor and Accessories A balanced load is essential for all centrifuges An unbalanced load produces vibration and can damage the unit ALWAYS balance containers on opposite sides of the rotor NOTE A 2 gram load imbalance at a speed of 4600 RPM imparts force equivalent to 20 pounds 9 1 kg at rest Always...

Page 7: ...ions 1 5 and 10 14 or 7 3 and 16 12 3 Load six tubes at positions 1 9 5 and 14 18 10 or 7 9 3 and 12 18 16 4 Loading an odd number of tubes is not recommended Rotor Removal To remove the rotor 1 If your rotor has a dic remove this first by loosening the nut 2 Using the wrench provided loosen the rotor locking nut 3 Remove the wrench 4 Remove the locking nut 5 Lift the rotor off the shaft 3 2 Start...

Page 8: ...ther with its default radius if not explicitly entered to the program Recall Program 1 Press the PROGRAM key to enter program mode 2 Select the appropriate program number by entering the desired program number on the numeric key pad and press ENTER The program s set parameters will display 3 Press START to begin this run Lock Program Unlock Program To lock a program to prevent changes to the progr...

Page 9: ...lay indicates relative centrifugal force in units of g Use the numeric keys to change the set speed or force Select speed in increments of 50 RPM from 500 through 15 000 RPM depending on the max allowable speed limit for the particular rotor Select RCF from 500 21 000 xg depending on the max allowable RCF limit for the particular rotor in increments of 50 xg ROTOR RADIUS SPEED RPM RCF Press these ...

Page 10: ... 3 7 Diagnostic Messages and Error Codes The beeper sounds in these situations Two times on power up Three times at the end of a spin Five times when a warning occurs Diagnostics Diagnostic messages appear in place of the speed display in the following cases Message Description bAL bAL indicates an unbalanced rotor Open the cover to erase this message Verify that a balanced load is installed Inspe...

Page 11: ... Direction of Rotation The microprocessor discovered wrong direction of rotation during acceleration Warnings during a spin LId PFAIL and dIR error messages can occur during a spin In this case the rotor brakes or coasts to a stop and the run ends 4 Applications 4 1 Introduction This section describes the use of specific rotors and accessories More detailed information is shipped with the rotor or...

Page 12: ...6 2 pr x 6559E 4 x 100 ml sealed Round bottom 38x111 mm 4500 3940 17 4 2 pr x 6557E 8 x 50 mL sealed Corning 8240 50 Thermo Electron 2997 4500 3980 17 6 2 pr x 6556E 8 x 50 mL sealed Falcon Corning conical 4500 3980 17 6 2 pr x 6560E 12 x 25 30 mL sealed Universal 25 x 107mm 4500 3400 15 0 2 pr x 6568E 20 x 15 mL sealed Falcon Corning conical 4500 3980 17 6 2 pr x 6561E 20 x 10 ml sealed Kova Uris...

Page 13: ...earest 50 rpm or 50 xg Tube Maximum Radius Tube Adapter No x Vol ml Description RPM RCF Quantity x Cat No 24 x 1 5 2 0 mL 11x54 mm microtube 15000 21000 8 35 24 x 0 5 mL 8 x 54 mm microtube 15000 17432 6 93 24 x 0 5 mL 8 x 54 mm microtube 15000 18740 7 45 2 pr x 5763E 24 x 0 4 mL 6 x 50 mm microtube 15000 21000 8 35 2 pr x 5764E 24 x 0 25 mL 6 x 50 mm microtube 15000 18240 7 25 2 pr x 5764E Tube M...

Page 14: ...700 RPM 4 000 x 925 3 700 Specific gravities greater than 3 0 This table is based on the formula Use the same formula to compute derating factors for specific gravities greater than 3 0 S0 is the maximum specific gravity allowed before derating 1 2 Sa is the actual specific gravity of the sample CAUTION Do not exceed the rated speed or specific gravity Higher speeds or specific gravities will impo...

Page 15: ...irs For Oxidizing Acids see Oxidizing Agents strong Derating Table for Microplate Rotor Load per Carrier grams Max Speed rpm 320 3650 340 3550 360 3450 380 3400 400 3300 450 3100 500 2950 600 2700 Greater than 600 do not use Plastic Metal Other POLYALLOMER POLYCARBONATE POLYETHYLENE POLYPROPYLENE POLYURETHANE MODIFIED PHENYLENE OXIDE NORYL ACETAL HOMOPOLYMER DELRIN ACETAL COPOLYMER CELCON NYLON PO...

Page 16: ...EL ALUMINUM MAGANESE BRONZE MAGNESIUM RUBBER BUNA N VITON PHENOLIC FIBER PT PAINTED SURFACE Mechanical Autoclave S M U S M U S S S U S S S S S S S M S M Ethylene Oxide Gas S S S S S S S S S S S S S S S U U S S S Dry Heat 2Hrs 160 C U U U U U U U U U U S S U S S U U U U U Chemical Ethanol S S S S U S S S U M S S S S S S S S S S 40 Formalin S S S S U S S S S U S S S S S S U S S S Methanol S M S S M ...

Page 17: ...OM6464K Rev 4 11 January 2004 Page 17 of 24 4 6 RCF Nomograph ...

Page 18: ... remove the corrosion discontinue use of the product and inform technical service 5 3 Storage Keep the Unit Dry Store parts on a soft surface to avoid damage Rotors and other parts should be clean and dry Store them open to the air not in a plastic bag so any residual moisture evaporates Face the parts upward to avoid moisture retention in the cavities 5 4 Decontamination Procedures WARNING WARNIN...

Page 19: ...y 9 to 40 C by 1 Rotation Counterclockwise RPM 500 15 000 by 50 RPM RCF 50 21 000xg by 50 xg Spin Duration 0 99 min 59 sec by 1 min and or 1 sec HOLD MODE Momentary Spin Brake Active Electric 3 profiles Repeatability Temperature Control IEC Centra CL5R only 2 C within range of 4 C to ambient Rotation Accuracy within 10 RPM Timer Microprocessor controlled Accuracy within 1 sec System Components Mot...

Page 20: ...OM6464K Rev 4 11 January 2004 Page 20 of 24 ...

Page 21: ...ANY PERSON OR ENTITY OTHER THAN THERMO WITHOUT THERMO S PRIOR WRITTEN APPROVAL OR ANY USE OF REPLACEMENT PARTS NOT SUPPLIED BY THERMO SHALL IMMEDIATELY VOID AND CANCEL ALL WARRANTIES WITH RESPECT TO THE AFFECTED PRODUCTS THE OBLIGATIONS CREATED BY THIS SECTION TO REPAIR OR REPLACE A NON CONFORMING PRODUCT SHALL BE THE SOLE REMEDY OF PURCHASER IN THE EVENT OF A NON CONFORMING PRODUCT EXCEPT AS EXPR...

Page 22: ...OM6464K Rev 4 11 January 2004 Page 22 of 24 Notes ...

Page 23: ...OM6464K Rev 4 11 January 2004 Page 23 of 24 ...

Page 24: ...2778 1101 Fax 91 22 2778 1103 Bangaalore India 81 45 453 9122 Fax 81 45 453 9222 Yokohama City Japan International Sales Office Locations Bioscience Technologies Thermo Electron Corporation 866 9 THERMO 866 984 3766 Molecular Biology info molbio thermo com Sample Preparation info sampleprep thermo com New Labs newlabs thermo com Services services biosciencetech thermo com Laboratory Pipetting and ...

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