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Thermo 6464K, Instruction Manual

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Analyze Detect Measure Control™

IEC Centra CL5 and CL5R

Centrifuges

OM6464K Rev. 4   11 January 2004

CL5: VENTILATED

 Cat. No.6464K: 120 VAC, 60Hz

 Cat. No.6465K: 220/240 VAC, 50/60Hz

CL5R: REFRIGERATED

 Cat. No.6466K: 120 VAC, 60Hz

 Cat. No.6467K: 220/240 VAC, 50/60Hz

Instruction Manual

®

Summary of Contents for 6464K

Page 1: ...EC Centra CL5 and CL5R Centrifuges OM6464K Rev 4 11 January 2004 CL5 VENTILATED Cat No 6464K 120 VAC 60Hz Cat No 6465K 220 240 VAC 50 60Hz CL5R REFRIGERATED Cat No 6466K 120 VAC 60Hz Cat No 6467K 220 240 VAC 50 60Hz Instruction Manual ...

Page 2: ......

Page 3: ... 1 Rotor and Accessories 6 3 2 Starting and Stopping a Run 7 3 3 Stored Programs 8 3 4 Rotor Recognition System 8 3 5 Refrigeration IEC Centra CL5R only 8 3 6 Front Panel 9 3 7 Diagnostic Messages and Error Codes 10 4 Applications 11 4 1 Introduction 11 4 2 Speed and Force Tables 12 4 3 Derating Tables for Dense Samples 14 4 4 Chemical Resistance Table 15 4 5 Decontamination Table 16 4 6 RCF Nomog...

Page 4: ...OM6464K Rev 4 11 January 2004 Page 4 of 24 ...

Page 5: ...nual is written for centrifuge operators In addition to operation information it contains a few basic troubleshooting techniques and a chapter on maintenance This manual is not a guide for servicing centrifuge units Warnings Cautions and Notes WARNING WARNING A WARNING advises against certain actions or situations that could result in personal injury CAUTION A CAUTION advises against actions or si...

Page 6: ...its new location ensure that the suction cups adhere correctly to the work surface 3 Operation 3 1 Rotor and Accessories A balanced load is essential for all centrifuges An unbalanced load produces vibration and can damage the unit ALWAYS balance containers on opposite sides of the rotor NOTE A 2 gram load imbalance at a speed of 4600 RPM imparts force equivalent to 20 pounds 9 1 kg at rest Always...

Page 7: ...ions 1 5 and 10 14 or 7 3 and 16 12 3 Load six tubes at positions 1 9 5 and 14 18 10 or 7 9 3 and 12 18 16 4 Loading an odd number of tubes is not recommended Rotor Removal To remove the rotor 1 If your rotor has a dic remove this first by loosening the nut 2 Using the wrench provided loosen the rotor locking nut 3 Remove the wrench 4 Remove the locking nut 5 Lift the rotor off the shaft 3 2 Start...

Page 8: ...ther with its default radius if not explicitly entered to the program Recall Program 1 Press the PROGRAM key to enter program mode 2 Select the appropriate program number by entering the desired program number on the numeric key pad and press ENTER The program s set parameters will display 3 Press START to begin this run Lock Program Unlock Program To lock a program to prevent changes to the progr...

Page 9: ...lay indicates relative centrifugal force in units of g Use the numeric keys to change the set speed or force Select speed in increments of 50 RPM from 500 through 15 000 RPM depending on the max allowable speed limit for the particular rotor Select RCF from 500 21 000 xg depending on the max allowable RCF limit for the particular rotor in increments of 50 xg ROTOR RADIUS SPEED RPM RCF Press these ...

Page 10: ... 3 7 Diagnostic Messages and Error Codes The beeper sounds in these situations Two times on power up Three times at the end of a spin Five times when a warning occurs Diagnostics Diagnostic messages appear in place of the speed display in the following cases Message Description bAL bAL indicates an unbalanced rotor Open the cover to erase this message Verify that a balanced load is installed Inspe...

Page 11: ... Direction of Rotation The microprocessor discovered wrong direction of rotation during acceleration Warnings during a spin LId PFAIL and dIR error messages can occur during a spin In this case the rotor brakes or coasts to a stop and the run ends 4 Applications 4 1 Introduction This section describes the use of specific rotors and accessories More detailed information is shipped with the rotor or...

Page 12: ...6 2 pr x 6559E 4 x 100 ml sealed Round bottom 38x111 mm 4500 3940 17 4 2 pr x 6557E 8 x 50 mL sealed Corning 8240 50 Thermo Electron 2997 4500 3980 17 6 2 pr x 6556E 8 x 50 mL sealed Falcon Corning conical 4500 3980 17 6 2 pr x 6560E 12 x 25 30 mL sealed Universal 25 x 107mm 4500 3400 15 0 2 pr x 6568E 20 x 15 mL sealed Falcon Corning conical 4500 3980 17 6 2 pr x 6561E 20 x 10 ml sealed Kova Uris...

Page 13: ...earest 50 rpm or 50 xg Tube Maximum Radius Tube Adapter No x Vol ml Description RPM RCF Quantity x Cat No 24 x 1 5 2 0 mL 11x54 mm microtube 15000 21000 8 35 24 x 0 5 mL 8 x 54 mm microtube 15000 17432 6 93 24 x 0 5 mL 8 x 54 mm microtube 15000 18740 7 45 2 pr x 5763E 24 x 0 4 mL 6 x 50 mm microtube 15000 21000 8 35 2 pr x 5764E 24 x 0 25 mL 6 x 50 mm microtube 15000 18240 7 25 2 pr x 5764E Tube M...

Page 14: ...700 RPM 4 000 x 925 3 700 Specific gravities greater than 3 0 This table is based on the formula Use the same formula to compute derating factors for specific gravities greater than 3 0 S0 is the maximum specific gravity allowed before derating 1 2 Sa is the actual specific gravity of the sample CAUTION Do not exceed the rated speed or specific gravity Higher speeds or specific gravities will impo...

Page 15: ...irs For Oxidizing Acids see Oxidizing Agents strong Derating Table for Microplate Rotor Load per Carrier grams Max Speed rpm 320 3650 340 3550 360 3450 380 3400 400 3300 450 3100 500 2950 600 2700 Greater than 600 do not use Plastic Metal Other POLYALLOMER POLYCARBONATE POLYETHYLENE POLYPROPYLENE POLYURETHANE MODIFIED PHENYLENE OXIDE NORYL ACETAL HOMOPOLYMER DELRIN ACETAL COPOLYMER CELCON NYLON PO...

Page 16: ...EL ALUMINUM MAGANESE BRONZE MAGNESIUM RUBBER BUNA N VITON PHENOLIC FIBER PT PAINTED SURFACE Mechanical Autoclave S M U S M U S S S U S S S S S S S M S M Ethylene Oxide Gas S S S S S S S S S S S S S S S U U S S S Dry Heat 2Hrs 160 C U U U U U U U U U U S S U S S U U U U U Chemical Ethanol S S S S U S S S U M S S S S S S S S S S 40 Formalin S S S S U S S S S U S S S S S S U S S S Methanol S M S S M ...

Page 17: ...OM6464K Rev 4 11 January 2004 Page 17 of 24 4 6 RCF Nomograph ...

Page 18: ... remove the corrosion discontinue use of the product and inform technical service 5 3 Storage Keep the Unit Dry Store parts on a soft surface to avoid damage Rotors and other parts should be clean and dry Store them open to the air not in a plastic bag so any residual moisture evaporates Face the parts upward to avoid moisture retention in the cavities 5 4 Decontamination Procedures WARNING WARNIN...

Page 19: ...y 9 to 40 C by 1 Rotation Counterclockwise RPM 500 15 000 by 50 RPM RCF 50 21 000xg by 50 xg Spin Duration 0 99 min 59 sec by 1 min and or 1 sec HOLD MODE Momentary Spin Brake Active Electric 3 profiles Repeatability Temperature Control IEC Centra CL5R only 2 C within range of 4 C to ambient Rotation Accuracy within 10 RPM Timer Microprocessor controlled Accuracy within 1 sec System Components Mot...

Page 20: ...OM6464K Rev 4 11 January 2004 Page 20 of 24 ...

Page 21: ...ANY PERSON OR ENTITY OTHER THAN THERMO WITHOUT THERMO S PRIOR WRITTEN APPROVAL OR ANY USE OF REPLACEMENT PARTS NOT SUPPLIED BY THERMO SHALL IMMEDIATELY VOID AND CANCEL ALL WARRANTIES WITH RESPECT TO THE AFFECTED PRODUCTS THE OBLIGATIONS CREATED BY THIS SECTION TO REPAIR OR REPLACE A NON CONFORMING PRODUCT SHALL BE THE SOLE REMEDY OF PURCHASER IN THE EVENT OF A NON CONFORMING PRODUCT EXCEPT AS EXPR...

Page 22: ...OM6464K Rev 4 11 January 2004 Page 22 of 24 Notes ...

Page 23: ...OM6464K Rev 4 11 January 2004 Page 23 of 24 ...

Page 24: ...2778 1101 Fax 91 22 2778 1103 Bangaalore India 81 45 453 9122 Fax 81 45 453 9222 Yokohama City Japan International Sales Office Locations Bioscience Technologies Thermo Electron Corporation 866 9 THERMO 866 984 3766 Molecular Biology info molbio thermo com Sample Preparation info sampleprep thermo com New Labs newlabs thermo com Services services biosciencetech thermo com Laboratory Pipetting and ...

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