Thermo Scientific thermoscientific Vanquish UHPLC System, Operating Manual

Page: 26 / 148

2 • Safety
Page 26 UHPLC System
Operating Manual
2.4.2 Allowed pH Ranges
Allowed pH ranges (standard system configuration):
System
(Standard
Configuration) Allowed
pH ranges Remarks
Vanquish Core 1-13 • pH values of 2 or less : The application time should
be as short as possible. Flush the system
thoroughly after these applications.
• pH values higher than 9.5 with optical detectors :
Avoid using mobile phases with a pH value higher
than 9.5 together with optical detectors. This can
impair the functionality and optical performance
of the detector flow cell.
• pH values higher than 12 : May affect
electrochemical detection. Before using highly
alkaline solvents for flushing the system,
disconnect the detector from the system.
• Mobile phases containing ammonium hydroxide : In
rare cases, a shortened lifetime of reversed-phase
(UHMW-PE) piston seals has been observed with
high pH, ammonium hydroxide containing mobile
phases and prolonged exposure.
Vanquish
Horizon
Vanquish Flex
2-12
2.4.3 Allowed Concentrations
Allowed concentrations (standard system configuration):
System
(Standard
Configuration) Chloride Buffer Remarks
Vanquish Core 0.1 mol/L
or less 1 mol/L or
less • High chloride concentration :
The application time should be
as short as possible. Flush the
system thoroughly after these
applications.
• Mobile phases containing
ammonium hydroxide
: In rare
cases, a shortened lifetime of
reversed-phase (UHMW-PE)
piston seals has been observed
with high pH, ammonium
hydroxide containing mobile
phases and prolonged
exposure.
Vanquish Horizon
Vanquish Flex
1 mol/L or
less -