8
•
Troubleshooting
UHPLC System
Operating Manual
Page 119
8.2.4
Flow
No flow
Possible Cause
Remedial Action
Leak in system
Find and eliminate the leak.
Inlet and/or outlet
check valve
contaminated or
defective
Clean the inlet check valve or outlet check valve, and replace the check valve if
necessary (refer to
Pump Operating Manual
).
Air trapped in pump
heads
Purge the pump (refer to
Pump Operating Manual
) and check in the Chromeleon
software whether the degasser has reached the operating vacuum
(Degasser Vacuum = OK).
Air trapped in
autosampler flow path
Perform a wash cycle (refer to
Split Sampler Operating Manual
).
Column temperature
too high – eluent
evaporating
Select a lower column temperature (refer to
Column Compartment Operating
Manual
).
Flow fluctuations
Possible Cause
Remedial Action
Pump inlet path
clogged
Check the solvent inlet lines, solvent line filters, and solvent selectors/proportioning
valve, for example, for signs of clogging.
Air trapped in pump
inlet path
•
Purge the pump.
•
Inspect the degasser for indications for leakage. Check in the Chromeleon
software whether the degasser has reached the operating vacuum
(Degasser Vacuum = OK). Degassing may not work properly if the flow rate is too
high.
•
Inspect the capillary and solvent line connections for leakage; tighten loose fitting
connections.
Inlet and/or outlet
check valve
contaminated or
defective
Clean the inlet check valve or outlet check valve, and replace the check valve if
necessary (refer to
Pump Operating Manual
).
Piston seals not sealing
tightly
Replace the piston seals (refer to
Pump Operating Manual
).
Summary of Contents for thermoscientific Vanquish UHPLC System
Page 4: ...Contacting Us Page 4 UHPLC System Operating Manual ...
Page 28: ...2 Safety Page 28 UHPLC System Operating Manual ...
Page 34: ...3 System Overview Page 34 UHPLC System Operating Manual ...
Page 38: ...4 Unpacking Page 38 UHPLC System Operating Manual ...
Page 82: ...5 Installation Page 82 UHPLC System Operating Manual ...
Page 100: ...6 Operation Page 100 UHPLC System Operating Manual ...
Page 112: ...7 Maintenance and Service Page 112 UHPLC System Operating Manual ...
Page 128: ...8 Troubleshooting Page 128 UHPLC System Operating Manual ...
Page 132: ...9 Specifications Page 132 UHPLC System Operating Manual ...
Page 144: ...11 Appendix Page 144 UHPLC System Operating Manual ...