4
Xcalibur Diagnostics for the PDA Detector
Calibrating the PDA Detector
32
Surveyor PDA Plus Detector Hardware Manual
Thermo Scientific
To perform a wavelength calibration with Xcalibur
1. Pump HPLC-grade methanol at 1 mL/min through the flow cell.
2. Turn on both lamps and wait one hour for the lamps to equilibrate:
a. In the view bar of the Instrument Setup window, click the
Surveyor PDA
Plus
button. The Surveyor PDA Plus Instrument Setup window appears.
b. From the menu bar, choose
Surveyor PDA Plus > Direct Control
.
The Surveyor
PDA Plus Direct Control dialog box appears.
c.
Click the
Configuration
tab. The Configuration page appears.
d. In the Deuterium Lamp area, turn on the deuterium lamp by clicking
Lamp On
.
e.
In the Tungsten Lamp area, turn on the tungsten lamp by clicking
Lamp On
.
3. After the detector has reached a stable temperature (approximately one hour after you
turn on the lamps), start the Wavelength Calibration wizard, and follow the instructions
on each page:
a. Click the
Calibration
tab. The Calibration page appears (see
).
Figure 25.
Calibration page
