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Chemical Compatibility Chart

Thermo Scientific

Sorvall Legend XF / XFR

A-3

Ethylene Glycol

S S S S S S S S S S S S - S U S S S S S S S S M S M S

Ethylene Oxide Vapor

S - U - - U - - S U - S - S M - - S S S U S U S S S U

Ficoll-Hypaque

M S S - S S S - S S S S - S S - S S S S S S S M S S S

Hydrofluoric Acid (10%)

U U U M - - U - - U U S - S M U S S S S M S U U U - -

Hydrofluoric Acid (50%)

U U U U - - U - - U U U U S U U U S S M M S U U U - M

Hydrochloric Acid (conc.)

U U U U - U U M - U M U U M U U U - S - U S U U U - -

Formaldehyde (40%)

M M M S S S S M S S S S M S S S U S S M S S S M S M U

Glutaraldehyde

S S S S - - S - S S S S S S S - - S S S - - S S S - -

Glycerol

M S S - S S S S S S S S S S S S - S S S S S S S S S S

Guanidine Hydrochloride

U U S - S S S - S S S S S S S - - S S S S S S U S S S

Haemo-Sol

S S S - - - S - S S S S S S S - - S S S S S S S S S S

Hexane

S S S - S S S - S S U S U M U S S U S S M S U S S U S

Isobutyl Alcohol

- - M U - - S S - U - S U S S M S S S - S S S - S - S

Isopropyl Alcohol

M M M U S S S S S U S S U S U M S S S S S S S M M M S

Iodoacetic Acid

S S M - S S S - S M S S M S S - M S S S S S M S S M M

Potassium Bromide

U S S - S S S - S S S S S S S S S S S - S S S M S S S

Potassium Carbonate

M U S S S S S - S S S S S S U S S S S S S S S S S S S

Potassium Chloride

U S S - S S S S S S S S S S S - S S S S S S S U S S S

Potassium Hydroxide (5%)

U U S S S S M - S S S S - S U S S S S S S S M U M S U

Potassium Hydroxide (conc.)

U U M U - - M - M S S - U M U U U S M - M U - U U - U

Potassium Permanganate

S S S - S S S - S S S U S S S M - S M S U S S M S U S

Calcium Chloride

M U S S S S S S S S S S S S M S - S S S S S S M S S S

Calcium Hypochlorite

M - U - S M M S - M - S - S M S - S S S M S M U S - S

Kerosene

S S S - S S S U S M U S U M M S - M M M S S U S S U S

Sodium Chloride (10%)

S - S S S S S S - - - S S S S S - S S S S - S S M - S

Sodium Chloride (sat'd)

U - S U S S S - - - - S S S S S - S S - S - S S M - S

Carbon Tetrachloride

U U M S S U M U S U U S U M U S S M M S M M M M U S S

Aqua Regia

U - U U - - U - - - - - U U U U U U U - - - - - S - M

Solution 555 (20%)

S S S - - - S - S S S S S S S - - S S S - S S S S S S

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Summary of Contents for Sorvall Legend XF

Page 1: ...Thermo Scientific Sorvall Legend XF XFR Centrifuge Instruction Manual 50156637 b 03 2020 Visit us online to register your warranty thermofisher com labwarranty ...

Page 2: ...s Supply 1 4 Rotor Selection 1 5 Before use 2 1 Before Setting up 2 2 Setting up 2 2 Transporting the Centrifuge 2 4 Location 2 4 Aligning the Centrifuge 2 4 Mains Connection 2 5 Storage 2 6 Control Panel 3 1 Control Panel 3 2 Keys 3 2 Operation 4 1 Switch on Centrifuge 4 2 Lid Opening 4 2 Close Lid 4 2 Rotor Installation 4 2 Entering Parameters 4 4 Centrifugation 4 10 Temperature Adaptation durin...

Page 3: ...3 Maintenance and Care 6 1 Cleaning Intervalls 6 2 Cleaning 6 2 Disinfection 6 3 Decontamination 6 4 Autoclaving 6 5 Service of Thermo Fisher Scientific 6 5 Trouble Shooting 7 1 Mechanical Emergency Door Release 7 2 Troubleshooting by User 7 3 When to contact Customer Service 7 5 Chemical Compatibility Chart A 1 ...

Page 4: ...e to follow the instructions and safety information in this instruction manual will result in the expiration of the sellers warranty Scope of Supply If any parts are missing please contact your nearest Thermo Fisher Scientific representative Intended Use This centrifuge is designed to separate sample mixtures of different densities like chemicals environmental samples and other non human body samp...

Page 5: ...is to be used for its intended use only Plug the centrifuge only into sockets which have been properly grounded Do not move the centrifuge while it is running Do not lean on the centrifuge Use only rotors and accessories for this centrifuge which have been approved by Thermo Fisher Scientific Exceptions to this rule are commercially available glass or plastic centrifuge tubes provided they have be...

Page 6: ...zardous substances Do not centrifuge explosive or flammable materials or substances which could react violently with one another The centrifuge is neither inert nor protected against explosion Never use the centrifuge in an explosion prone environment Do not centrifuge inflammable substances Do not centrifuge toxic or radioactive materials or any pathogenic micro organisms without suitable safety ...

Page 7: ...eneral hazards CAUTION means that material damage could occur WARNING means that injuries or material damage or contamination could occur This symbol refers to biological hazards Observe the information contained in the instruction manual to keep yourself and your environment safe ...

Page 8: ...duction and Description Contents Characteristics of the Sorvall Legend XF XFR on page 1 2 Technical Data on page 1 3 Directives Standards and Guidelines on page 1 4 Functions and Features on page 1 5 Mains Supply on page 1 5 Rotor Selection on page 1 6 ...

Page 9: ... the entry mode These settings can be changed even while the centrifuge is running you can also cent With the help of the PULSE key rifuge a sample for just a few seconds if called for The Sorvall Legend XF XFR is equipped with various safety features The housing and rotor chamber consist of steel plate the interior of armour steel while the front panel is made of high impact resistant plastic The...

Page 10: ...30V 120V 230V 120V 6653 BTU h 4776 BTU h 5800 BTU h 4776 BTU h IP 20 20 running time unlimited unlimited max Speed nmax 15200 rpm depending on the rotor 15200 rpm depending on the rotor min Speed nmin 300 rpm 300 rpm maximum RCF value at nmax 25314 x g 25314 x g maximum kinetic energy 62 5 kJ 62 5 kJ noise level at maximum speed 64 dB A 64 dB A temperature setting range 10 to 40 Dimensions ventila...

Page 11: ...ns and Features Component Function Description Features Galvanized steel chassis with armoured plating Structure Housing Stainless steel varnished steel Chamber Induction drive without carbon brushes Drive Easy to clean keypad and display surface Keys and display Controls Microprocessor controlled The most recent data is saved Internal memory Functions RCF selection temperature control pretemp wit...

Page 12: ...more information visit our website at http www thermofisher com TX 750 7500 3180 with round buckets 7500 3608 with rectangular buckets 7500 3614 with microplate carrier 7500 3617 BIOshieldTM 1000A 7500 3182 HIGHPlateTM 6000 7500 3606 Fiberlite H3 LV 7500 3665 Fiberlite F14 6 x 250LE 7500 3662 Fiberlite F15 6 x 100y 7500 3698 Fiberlite F13 14 x 50cy 7500 3661 Fiberlite F15 8 x 50cy 7500 3663 Fiberl...

Page 13: ...rvall Legend XF XFR 2 1 2 Before use Contents Before Setting up on page 2 2 Transporting the Centrifuge on page 2 4 Location on page 2 4 Aligning the Centrifuge on page 2 4 Mains Connection on page 2 5 Storage on page 2 6 ...

Page 14: ...company and Thermo Fisher Scientific immediately if any damage is discovered 2 Remove the packaging 3 Check the order for completeness see Scope of Supply on page iii If the order is incomplete please contact Thermo Fisher Scientific Setting up 1 Place the pallet with the centrifuge so that you have at leased 1 5 m room in front of the pallet 2 Release the rails from the pallet ...

Page 15: ...Screw the rails onto the pallet in front of the centrifuge 4 Remove the protectors that locate the centrifuge on the pallet Release the castors Use several people in order to roll the centrifuge off the pallet 5 Fix the castors once the centrifuge is in its final position ...

Page 16: ...and free of resonance for example a level laboratory bench The supporting structure must be suitable for horizontal setup of the centrifuge The centrifuge should not be exposed to heat and strong sunlight The set up location must be well ventilated at all times Aligning the Centrifuge The horizontal alignment of the centrifuge must be checked every time after moving it to a different location The ...

Page 17: ... switch on the back press 0 2 Plug the centrifuge into grounded electrical sockets only 3 Check whether the cable complies with the safety standards of your country 4 Make sure that the voltage and frequency correspond to the figures on the rating plate 5 Establish the connection to the power supply with the connecting cable Main Power Mains plug ...

Page 18: ...tific Storage Before storing the centrifuge and the accesories it must be cleanedand if necessary desinfected and decontaminated Store the centrifuge in a clean dust free location Be sure to place the centrifuge on its feet Avoid direct sunlight Fix the castors ...

Page 19: ...Thermo Scientific Sorvall Legend XF XFR 3 1 3 Control Panel Contents Control Panel on page 3 2 Keys on page 3 2 ...

Page 20: ...ble only when device is switched on Emergency release see Mechanical Emergency Door Release on page 7 2 Pulse By pressing the key PULSE the centrifuge starts immediately and accelerates up to the end speed Releasing the key initiates a stopping process at the highest braking curves Directory Use the directory key in order to modify the displayed value Bucket Use the bucket key in order to have all...

Page 21: ...on page 4 2 Lid Opening on page 4 2 Rotor Installation on page 4 2 Entering Parameters on page 4 4 Centrifugation on page 4 10 Temperature Adaptation during Standstill on page 4 11 Short term Centrifugation on page 4 11 Removing the Rotor on page 4 12 Aligning the Centrifuge on page 4 13 ...

Page 22: ...lay shows The top line shows the message Lid open The bottom line shows the values for the last run Lid Opening 1 Press the OPEN key Close Lid 2 Close the lid by pressing down on it lightly in the middle or on both sides of it Two locks close the lid completely Rotor Installation The approved rotors for the Sorvall Legend XF XFR are listed in section Rotor Selection on page 1 6 Use only the rotors...

Page 23: ...tor is properly installed by lifting it slightly on the handle If the rotor can be pulled up then it must be reclamped to the centrifuge spindle CAUTION Unapproved or incorrectly combined accessories can cause serious damage to the centrifuge CAUTION Do not force the rotor onto the centrifuge spindle If the rotor is very light then it may be necessary to press it onto the centrifuge spindle with a...

Page 24: ...desired profile by entering a digit between 1 9 on the numeric pad Curve number 1 is the slowest and curve number 9 the fastest 3 Confirm your entry by pressing ENTER Braking curve 1 Press the ACC DEC key twice in order to open the braking curve selection menu The display shows the message Set deceleration 2 Select the desired curve by entering a digit between 1 9 on the numeric pad Curve number 1...

Page 25: ...ll be corrected automatically if the resulting speed is less than 300 rpm This is because 300 rpm is the lowest selectable speed Explanation of RCF Value The relative centrifugal force is given as a multiple of the force of gravity g It is a unitless numerical value which is used to compare the separation or sedimentation capacity of various devices since it is independent of the type of device On...

Page 26: ...ue running until you stop it manually with the STOP key Run time mode Use the TOGGLE key to set the run time counter start AT START When AT START is lit the running time will be counted right from pressing the START key AT SPEED When AT SPEED is lit the running time will be counted once the preset speed or the preset RCF value respectively have been reached In this case the selected acceleration c...

Page 27: ...s 2 Enter the desired temperature using the numeric pad The digits show in sequential order 3 Confirm your entry by pressing ENTER Your entry will be automatically confirmed if you do not press any key for 5 seconds Prewarming or precooling the centrifuge For setting the pretemp value for the centrifuge proceed as follows 1 Hold down the TEMPERATURE key for a minimum of three seconds to open the t...

Page 28: ...our digits of the bucket catalog number 1 Press the BUCKET key The display shows the following message 2 Press the BUCKET key repeatedly until the bucket being used is displayed Your entry will be automatically confirmed if you do not press any key for 5 seconds Bucket radius Having entered the bucket code you may want to specify the radius When you use adaptors you will have to specify the radius...

Page 29: ...ting a program Programs can be stored and retrieved later by means of the assigned number 1 Enter the program parameters as described above 2 Press the program memory key to load any of the programs 1 5 Press the DIRECTORY key in order to load any of the programs 1 99 by means of the numeric pad Enter the program number and confirm with ENTER key 3 You may want to enter a passcode to protect the d...

Page 30: ...two hours ago this message will apear in the display Check the castor breaks before pressing the START key again Starting centrifuge program Press the START key on the control panel The centrifuge accelerates to the pre set speed with the time display active If the speed setting is higher than the maximum permissible speed or RCF value for the particular rotor then the display will show the messag...

Page 31: ...lerated at the designated rate The message END will illuminate and after pressing the key OPEN the lid will open and you can remove the centrifuged material Temperature Adaptation during Standstill The temperature cannot be adapted until the rotor has been positively identified For this the centrifuge must have accelerated to over 500 rpm The speed display will then show END When the rotor is not ...

Page 32: ...entrifuge spindle Make sure not to tilt the rotor while doing this Aerosol tight Rotors When using an aerosol tight lid the rotor can only be removed with the lid closed This is to protect you and the samples Figure 4 2 Auto LockTM lid for aerosol tight rotors CAUTION Rotors supplied with a lid for aerosol tight applications come with a mandrel which belongs to the Auto LockTM Be sure not to place...

Page 33: ...4 Operation Aligning the Centrifuge Thermo Scientific Sorvall Legend XF XFR 4 13 Aligning the Centrifuge 1 To turn off the centrifuge put the mains switch to 0 ...

Page 34: ...1 7 Deutsch English 1 Deutsch 2 Française 3 Español 4 Italiano 5 Nederlands 6 7 Росский STOP ENTER LCD powersave START no 0 yes 1 ENTER ENTER No Program passcodes 0547V01 0 1 4641V05 1 ENTER STOP ENTER Set lid passcode ENTER No Auto lid open ENTER ENTER 0547V01 Software ID 2368 Cycle count ENTER 1 Software and NVRAM identification example display ...

Page 35: ...ise press 0 no Press ENTER key to confirm Keypad beep Press 1 yes on the numeric pad if the centrifuge should make beep when pressing any key Otherwise press 0 no Press ENTER key to confirm LCD powersave Press 1 yes on the numeric pad if the centrifuge should enter a powdersave mode after the run Otherwise press 0 no Press ENTER key to confirm Lid passcode Press 1 yes on the numeric pad if the cen...

Page 36: ...ress 1 yes on the numeric pad if the centrifuge should open after the run Otherwise press 0 no Press ENTER key to confirm Software ID Here you fine the current software version Press ENTER key to confirm Cycle count Here you fine the current numbers of cycles Press ENTER key to confirm ...

Page 37: ...Legend XF XFR 6 1 6 Maintenance and Care Contents Cleaning Intervalls on page 6 2 Cleaning on page 6 2 Disinfection on page 6 3 Decontamination on page 6 4 Autoclaving on page 6 5 Service of Thermo Fisher Scientific on page 6 5 ...

Page 38: ...st never exceed 50 C since higher temperatures could damage the material and shorten the lifetime of the parts Use only disinfectants with a pH of 6 8 Dry aluminum parts off with a soft cloth After cleaning treat the entire surface of aluminum parts with corrosion protection oil 7000 9824 Also treat the cavities with oil Store the aluminum parts at room temperature or in a cold storage room with t...

Page 39: ...uring centrifugation The rotor chamber and the rotor should be treated preferably with a neutral disinfectant CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer users should check with the manufacturer that the proposed method will not damage the equipment CAUTION When cleaning do not allow liquids especially organic solvents to get on the dri...

Page 40: ...r cabinet at a maximum temperature of 50 C After cleaning treat the entire surface of aluminum parts with corrosion protection oil 7000 9824 Also treat the cavities with oil Tread the bold of the swing out rotor with bold grease 75003786 Decontamination Decantaminate the centrifuge immediately whenever radioactive material has spilled during centrifugation For general radioactive decontamination u...

Page 41: ...minum parts with corrosion protection oil 7000 9824 Also treat the cavities with oil Tread the bold of the swing out rotor with bold grease 75003786 Autoclaving 1 Before autoclaving clean rotor and accessories and described above 2 Place the rotor on a flat surface Rotors and adapter can beautoclaved at 121 C The maximum permissible autoclave cycle is 20 minutes at 121 C Service of Thermo Fisher S...

Page 42: ...entific Thermo Fisher Scientific offers inspection and service contracts for this work Any necessary repairs are performed for free during the warranty period and afterwards for a charge This is only valid if the centrifuge has only been mainained by a Thermo Fisher Scientific service technician ...

Page 43: ...Thermo Scientific Sorvall Legend XF XFR 7 1 7 Trouble Shooting Contents Mechanical Emergency Door Release on page 7 2 Troubleshooting by User on page 7 3 When to contact Customer Service on page 7 5 ...

Page 44: ...raking process lasts much longer than usual Proceed as follows 1 Make sure the rotor has stopped view port in the lid 2 Pull out the power supply plug 3 On the back of the housing are two black plastic plugs which you can pry out of the back plate with a screwdriver or a knife Pull the release cord attached to it at the same time to trigger the mechanical lid release The lid will open and the samp...

Page 45: ... braked Bucket code is undefined for the rotor detected check the set points for the given bucket code Is it permitted to use the current bucket in the rotor currently mounted Restart the centrifuge If an error message appears again inform Customer Service The centrifuge cannot be operated Unapproved rotor The run does not start or the centrifuge runs down without being braked Rotor code is not in...

Page 46: ...rifuge If an error message appears again inform Customer Service The centrifuge cannot be operated E 13 The run does not start or the centrifuge runs down without being braked The check sum in the data memory is incorrect The software corrects errors automatically Check the values of the set point settings etc Restart the centrifuge If an error message appears again inform Customer Service Tempera...

Page 47: ...e centrifuge If an error message appears again inform Customer Service E 33 The centrifuge cannot be operated The run does not start or the centrifuge runs down without being braked Overpressure in the refrigeration unit Clean the air inlet for the condenser Restart the centrifuge If an error message appears again inform Customer Service E 34 E 36 The centrifuge cannot be operated The run does not...

Page 48: ...to contact Customer Service 7 6 Sorvall Legend XF XFR Thermo Scientific 3 Press and hold the ENTER key until the following message is displayed 4 Communicate the software version to the service technician Software ID XXXXXXXX ...

Page 49: ...lyl Alcohol U S S S S M S S S M S S Aluminum Chloride U U S S S S U S S S S M S S S S S S S S S M U U S S Formic Acid 100 S M U U U S M U U S S U S U S U Ammonium Acetate S S U S S S S S S S S S S U S S S S S S S S S S Ammonium Carbonate M S U S S S S S S S S S S S U U S S S S S S M S S S Ammonium Hydroxide 10 U U S U S S M S S S S S S U M S S S S S S S S S M S Ammonium Hydroxide 28 U U S U S U M ...

Page 50: ...ethyl Ether S S U U S S S U S U U S U U U U U U U U U S S S S M U Diethyl Ketone S U U M S U S M U U U M M U S S U U Diethylpyrocarbonate S S U S S S S S U S U S U S S S M S S S S S S Dimethylsulfoxide S S U U S S S S U S S U S U U S S U U S S S S U U Dioxane M S U U S S M M S U U S U M U U M M M U S S S S U U Ferric Chloride U U S M S M S S S S M U S S Acetic Acid Glacial S S U U S S U M S U S U ...

Page 51: ... M S S S Potassium Carbonate M U S S S S S S S S S S S U S S S S S S S S S S S S Potassium Chloride U S S S S S S S S S S S S S S S S S S S S U S S S Potassium Hydroxide 5 U U S S S S M S S S S S U S S S S S S S M U M S U Potassium Hydroxide conc U U M U M M S S U M U U U S M M U U U U Potassium Permanganate S S S S S S S S S U S S S M S M S U S S M S U S Calcium Chloride M U S S S S S S S S S S S...

Page 52: ...5 U U M S S M U S S M S S S M S S S S M S S S M U S M S Sodium Iodide M S S S S S S S S S S S S S S S S S S M S S S Sodium Nitrate S S S S S S S S S S S S S S S S S S S S U S S S S Sodium Sulfate U S S S S S S S S S S S S S S S S S S S S S M S S S Sodium Sulfide S S S S S S S U U S S S M S Sodium Sulfite S S S S S S S M S S S S S S M S S S S S S S S S S Nickel Salts U S S S S S S S S S S S S S S S...

Page 53: ... Acid 50 U U U U S U U S M S U U M U U S S S S M S M U U M M Sulfuric Acid 10 M U U S S U U S S M U S S S S S S S S S S U U U S S Sulfuric Acid 50 M U U U S U U S S M U U S U U M S S S S S U U U M S Sulfuric Acid conc M U U U U U M M U U S U U U M S U M S U U U S Stearic Acid S S S M S S S S S S S S S S S S S M M S S S Tetrahydrofuran S S U U S U U M S U U S U U U M U U U U S U S S U U Toluene S S...

Page 54: ... S S Citric Acid 10 M S S M S S M S S S S S S S S S M S S S S S S S S S S 1Polyethyleneterephthalate CHEMICAL MATERIAL ALUMINUM ANODIC COATING for ALUMINUM BUNA N CELLULOSE ACETATE BUTYRATE POLYURETHANE ROTOR PAINT COMPOSITE Carbon Fiber Epoxy DELRIN ETHYLENE PROPYLENE GLASS NEOPRENE NORYL NYLON PET 1 POLYCLEAR CLEARCRIMP CCCLEARCRIMP POLYALLOMER POLYCARBONATE POLYESTER GLASS THERMOSET POLYTHERMID...

Page 55: ...ation of RCF Value 4 5 F Failure message 7 3 Features 1 5 I Imbalance indicator 4 10 Intended Use v K Keys 3 2 Kundendienst 6 5 7 5 L Lid Opening 4 2 Location 2 4 M Mains Connection 2 5 Mains Supply 1 5 2 6 Maintenance 6 1 O Operation 4 1 Overwriting a passcode protected program 4 9 Overwriting a program 4 9 P Passcode 4 9 Precautions iv Preface iii Preselecting RCF 4 4 Preselecting Speed 4 4 Pres...

Page 56: ... 4 Select Program 4 9 Short term Centrifugation 4 11 Starting centrifuge program 4 10 Stopping the centrifugation program 4 11 Storage 2 6 Switch on Centrifuge 4 2 T Technical Data 1 3 Temperature Adaptation 4 11 Transporting the Centrifuge 2 4 Trouble Shooting 7 1 ...

Page 57: ...ons terms and pricing are subject to change Not all products are available in all countries Please consult your local sales representative for details Shown pictures within the manual are examples and may differ considering the set parameters and language Pictures of the user interface within the manual are showing the English version as example Australia 61 39757 4300 Austria 43 1 801 40 0 Belgiu...

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