Thermo Scientific NanoDrop OneC, User Manual

Page: 43 / 144

3
Applications
Measure Custom
Thermo Scientific NanoDrop One
C
with NanoDropQC Software User Guide 39
Standards (Standard curve
methods only) Define the standards:
• Enter name and analyte concentration of each standard and a reference, if desired:
– Depending on the Curve Type setting, a standard curve can be generated
using two or more standards. (The software
allows a reference and up to 7
standards .)
– All reference and standards solutions should be in the same buffer used to
resuspend the samples plus the same volume of reagent added to the samples.
– First standard can be a reference measurement. The reference solution
should contain none of the analyte of interest. (The reference measurement is
not the same as a blank measurement.)
– Concentration values for standards can be entered in any order but the
standards must be measured in the order in which they were entered;
however, best practice dictates that standards be measured from the lowest
concentration of the standard analyte stock to the highest.
– Concentration range of the standards must cover the dynamic range of the
assay and the expected range of the unknown samples. Sample analyte
concentrations are not extrapolated beyond the concentration of the highest
standard.
• Select curve fit type.
Specify type of equation used to create standard curve from standard concentration
values. Available options:
– Linear: Draws the linear least squares line through all measured standards
(requires reference measurement and at least one standard)
–
Interpolation:
Draws a series of straight lines to connect all measured
standards (requires reference measurement ans at least one standard)
– 2
nd
order polynomial: Draws th 2
nd
order least squares polynomial using all
measured standards (requires reference measurement and at least standards)
–
3
rd
order polynomial:
Draws the 3
rd
order least squares polynomial using all
measured standards (requires reference measurement and at least three
standards)
Analysis wavelength
(Standard curve methods
only) Monitor absorbance at specified wavelength (enter the wavelength in nanometers).
Note : The specified wavelength must fall within the selected measurement range .
The measurement results or the concentration will be calculated automatically using
the absorbance value at the specified wavelength and applying the selected method
type (factor or standard curve).
Setting Available Options