Thermo Scientific NanoDrop One, User Manual

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4
Measure Microarray
Thermo Scientific NanoDrop One User Guide 31
Add or remove a dye
To add or remove a dye from the Dye1 or Dye2 drop-down list in Microarray Setup or
Proteins & Labels Setup :
– select or deselect corresponding checkbox
Add custom dye
– tap to show New Dye box
– enter unique Name for new dye (tap field to display keyboard, tap Done key to close
keyboard)
– select default Unit that will be used to display dye concentration
– enter dye’s Extinction Coefficient (or molar absorptivity constant) in L/mole-cm
(typically provided by dye manufacturer)
– specify Wavelength in nm (between 450 nm and 700 nm) that will be used to
measure dye’s absorbance
– specify dye’s correction values at 260 nm and 280 nm
– tap Add Dye
When a custom dye is selected before a measurement, the dye’s absorbance and
concentration values are reported and the corrections are applied to the measured sample
absorbance values, and to the resulting sample concentrations and purity ratios.
Edit custom dye
– tap to select custom dye
– tap
Note To determine dye correction values (if not available from dye manufacturer):
– use instrument to measure pure dye and note absorbance at 260 nm, 280 nm
and at analysis wavelength for dye (see above)
– calculate ratio of A
260
/A
dye wavelength
and enter that value for 260 nm
Correction
– calculate ratio of A
280
/A
dye wavelength
and enter that value for 280 nm
Correction
Tip Dyes pre-defined in the software cannot be edited.