Rev 3/09
T031
‐
TECHNICAL
BULLETIN
N a no D ro p
2000 /2000 c
Thermo Fisher Scientific - NanoDrop products Wilmington, Delaware USA Technical support: [email protected] 302-479-7707 www.nanodrop.com
Cleaning and Reconditioning
Pedestal Cleaning
1. Apply 3-5 ul of dH
2
0 on to the bottom pedestal. Never use a squirt bottle
to apply de-ionized water or any other liquid to the surface of the
instrument.
2. Lower the upper pedestal arm to form a liquid column; let it sit for
approximately 2-3 minutes.
3. Wipe away the water from both the upper and lower pedestal with a dry,
lint-free lab wipe.
−
Between measurements:
Wipe the sample from both the upper and lower pedestals with a clean, dry, lint-free lab wipe, to
prevent sample carryover and avoid residue buildup.
−
Between users:
A final cleaning of both measurement surfaces with dH
2
0 is recommended after the last sample measurement.
−
Additional cleaning:
Substitute 0.5M HCl for the dH
2
0 in the procedure above when proteins have dried on the pedestal.
−
Decontamination:
Use a sanitizing solution, such as a 0.5% solution of sodium hypochlorite (1:10 dilution of common
commercial bleach solution, freshly prepared), to decontaminate the measurement pedestals. Follow with 3- 5 ul of dH20.
The use of detergents or isopropyl alcohol is
not
recommended as they may un-condition the pedestal measurement surfaces. If a
solution containing either is used, it is important to follow with 3- 5 ul of dH20.
Pedestal Reconditioning
Use the instrument pedestal reconditioning kit, PR-1, as a rapid means of reconditioning the pedestals when the surface properties
have been compromised and liquid columns break during measurement.
1. Open the vial containing PR-1 and use the applicator provided in the kit to remove a pin-head sized amount of the compound.
2. Apply a very thin, even layer of PR-1 to the surface of the upper and lower pedestals and wait 30 seconds for thee PR-1 to dry.
3. Fold a clean, dry laboratory wipe into quarters and remove the PR-1 by aggressively rubbing the surface of the upper and lower
pedestals until all compound residue is removed. The appearance of a black residue on the laboratory wipe is normal.
4. Use canned air to remove excess lint from the diaphragm.
Test the effectiveness of the re-conditioning by pipetting a 1ul sample of
dH
2
O (using a calibrated 2 ul pipettor) onto the lower measurement pedestal.
The figure on the left is a flat bead of water on an unconditioned pedestal. The
figure on the right is a1ul sample of dH
2
O on a properly conditioned pedestal.
Cuvette Cleaning
•
For routine, daily cleaning use a dry lab swab or lab wipe to wick away any spills within or around the cuvette holder assembly.
•
The cuvette holder assembly may be cleaned of excess dust using canned air.
•
DO NOT squirt any liquid into the block as the liquid will flow into the instrument. If liquid does get into the block, it is
best to use a lab wipe or a lab cotton swab to absorb the liquid.
•
Follow the recommendations of the cuvette manufacturer for the cleaning and maintenance of cuvettes.
•
Cuvettes with scratches in the optical path should not be used. Ensure all optical surfaces are free of lint and fingerprints prior
to insertion in the NanoDrop 2000c cuvette holder.
For Technical Support contact us at 302-479-7707 or send an email to [email protected].
Properly Conditioned
Pedestal-water “Beads up”
Un-conditioned Pedestal-
water “Flattens out”
