3
Cuvette measurements with SkanIt Software
Creating a new measurement session
14
Thermo Scientific μDrop and μDrop Duo Plates User Manual
Thermo Fisher Scientific
Figure 12.
Cuvette inserted (μDrop Plate), showing the adjustment screw (1).
The screw should be adjusted according to the length of the cuvette with stopper. You do not need to
tighten the screw completely. This will allow convenient handling of the cuvettes in measurement
series.
Because of the measurement direction, possible air bubbles present in the liquid can cause severe errors
to the results. It is particularly important to follow good pipetting practices. The reverse pipetting
technique is strongly recommended.
1. Pipette the sample into the cuvette in a sufficient volume.
2. Close the stopper and check that there are no visible air bubbles or superfluous particles present in
the sample.
3. Place the cuvette into the cuvette slot and tighten the adjustment screw, see
. Ensure that
there are no air bubbles in the measurement location (white spot, see
).
Creating a new measurement session
User-made sessions
To create user-made measurement sessions for cuvette measurement, follow the normal SkanIt
Software procedure. However, there are certain special features to observe:
1. Plate Layout
Use the
Thermo Scientific μDrop Plate
or
Thermo Scientific μDrop Duo Plate
plate template in
SkanIt Software. Select the correct template from the
Change plate template
dropdown menu.
Conduct the cuvette measurement from position G11 of the μDrop or μDrop Duo Plate.