Thermo Electron Corporation
LTQ XL Getting Started
43
Chapter 3
Tuning and Calibrating
Automatically in the ESI/MS
Mode
This chapter provides information on how to tune and calibrate the LTQ
XL MS detector in the ESI/MS mode. For most applications, you tune and
calibrate in the ESI mode through automatic procedures. The procedures
use a calibration solution that is introduced into the MS detector in low
flow mode. The procedures properly tune and calibrate the MS detector for
ESI operation (refer to
for ESI operating parameter
guidelines). You need to calibrate the MS detector every one to three
months of operation for optimum performance over the entire mass range
of the detector.
To tune and calibrate your MS detector automatically in the ESI/MS mode,
you do the following:
• Infuse a low concentration calibration solution containing caffeine,
MRFA, and Ultramark 1621 into the ESI source by using the syringe
pump. (Refer to the section,
Setting Up the Syringe Pump for Tuning
.)
• Test the efficiency and stability of the spray of calibration solution into
the MS detector. You can observe the following singly-charged, positive
ions for caffeine, MRFA, and Ultramark 1621 in the Tune Plus window:
m/z
195, 524, 1222, 1522, and 1822.
• Tune the MS detector from the Tune Plus window to optimize
automatically the lenses.
• Calibrate the MS detector to adjust automatically the voltages of the
linear trap.
This chapter contains the following sections:
•
Setting Up the Syringe Pump for Tuning and Calibration
•
Setting Up the MS Detector in the Xcalibur Data System for Tuning
and Calibration
•
