Thermo Scientific ISQ, User Manual

Page: 33 / 152

2
Changing the Column
Replacing the Factory Installed Column
Thermo Scientific ISQ User Guide 11
Figure 4. Column Flowmeter Connector
b. Connect the flowmeter to the dedicated fitting on the column flowmeter connector.
c. If you have a TRACE 1310, select the Back Column or Front Column button in
the Configuration menu. Otherwise, perform the column evaluation through the
Chromatography Data System. See the TRACE 1300 and TRACE 1310 User Guide
for instructions.
d. Select Column and input the column’s physical characteristics.
e. If a pre-/post column is present, set the length and nominal internal diameter of the
pre-/post column in the same valid ranges for the column.
f. Start the column evaluation:
i. On the column page, click Column Evaluation .
ii. Connect a flowmeter to the column flowmeter connector as indicated above.
iii. Press Start . The inlet is pressurized to deliver a 5 mL calculated column flow.
iv. Once the pressure has stabilized, measure the flow out of the column flowmeter
connector. Enter the actual flow when prompted. The corrected column ID will
be displayed.
v. Select Apply and the K-factor will be adjusted to give the corrected column flow.
According to the physical characteristics of the column, the system calculates and
displays the relevant column K-factor. At the end of the routine, a message will
indicate that the evaluation was successful.
Note For the most reproducible results, you should conduct a more detailed
column evaluation. However, the following steps, while recommended, are not
required.
A B C
To flowmeter