Thermo Scientific ISQ, User Manual

Page: 32 / 152

2
Changing the Column
Replacing the Factory Installed Column
10 ISQ User Guide Thermo Scientific
d. Allow the column to purge for at least 10 minutes. If you used methanol to detect
column flow, remove column from methanol during purge time.
e. Insert the column into the fitting of the column flowmeter connector that blocks the
column flow.
15. Perform a column leak check:
a. On the TRACE 1310, select the Leak Check icon in the Maintenance menu.
Otherwise, perform the leak check through the Chromatography Data System. Refer
to the
TRACE 1300 and TRACE 1310 Series GC User Guide for instructions.
b. Start the leak check.
The split and purge valves of the selected channel are automatically closed, and the
channel is pressurized with carrier gas to the leak check setpoint.
The system monitors the pressure for one minute. If the pressure does not drop more
than the maximum allowed sensitivity value, then the leak check will pass. If the leak
check does not pass, use the leak detector to find and fix any leaks.
c. Repeat the leak check until no leaks are indicated.
16. Calibrate the carrier gas flow (column evaluation):
a. Carefully push the capillary column end into the flowmeter section of the column
flowmeter connector. See Figure 4 .
Note If you are using a TRACE GC Ultra or a FOCUS GC, refer to your GC
user documentation for instructions on performing a leak check.
Tip Leaks can be caused by not tightening the fitting on the column flowmeter
connector. Check the fitting before looking for the leak elsewhere.
CAUTION INSTRUMENT DAMAGE: Do not allow the column flowmeter
connector to exceed 80 °C (176 °F). Otherwise, it will melt and damage the
instrument.