Thermo Scientific Ion 520, User Manual

Page: 66 / 78

5.
Place a used sequencing chip in the chip clamp, then push the chip clamp in all the way to
engage.
6.
Close the instrument door, then tap Next .
Cleaning takes ~35 minutes to complete. On completion the instrument door automatically unlocks
and the chip and cartridge clamps disengage.
7.
Proceed to Chapter 5, “Initialize the sequencer”.
Perform an instrument reset run with an initialized
sequencer that is loaded with an unused Reagents
cartridge
Cleaning is normally automatically performed at completion of a sequencing run. If an Ion S5
™
Sequencer, Ion S5
™
XL Sequencer, or Ion GeneStudio
™
S5 Series Sequencer is initialized, an
instrument reset run is required to ensure proper cleaning before reinitialization in one of the following
situations.
• A sequencing run is not started within 24 hours after initialization.
• A sequencing run is not completed due to a power failure or an abort, and <200 flows occurred
before the stoppage.
Do NOT perform a manual cleaning with an unused, initialized Ion S5
™
ExT Sequencing Reagents
cartridge.
Note:
· If a power failure or abort occurs during the second of two runs started after a single initialization, a
manual cleaning is sufficient (see “Perform a manual cleaning of the sequencer”
on page 65 ).
· If the number of flows that occurred before a power failure or abort is unknown, perform an
instrument reset run.
To perform an instrument reset run, use the following procedure before reinitialization.
1.
In the instrument touchscreen main menu, tap Run .
The instrument door unlocks and the chip clamp disengages.
2.
Ensure that a used sequencing chip is in the chip clamp, then push the chip clamp in all the way to
engage.
3.
Close the instrument door, then tap Next .
4.
When prompted, select Planned Run (none) . Ensure that the Enable post-run clean checkbox is
selected, then tap Review .
5.
In the Select Run screen, tap Edit , then in the Detail screen set the number of flows to 200 .
Ensure that the Post-Run/Clean checkbox is selected, then tap Close .
6.
Tap Start run , then tap Accept to confirm that Post-Run Clean is enabled, and to start the run.
Appendix B
Supplemental procedures
Perform an instrument reset run with an initialized sequencer that is loaded with an unused Reagents cartridge
B
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Ion 520 ™ & Ion 530 ™ ExT Kit − Chef User Guide