Thermo Scientific ETD, Getting Started Manual

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6
Running an Xcalibur Experiment by Using ETD
Data Dependent NL MS
3
Experiment
Thermo Scientific MS/ETD System Getting Started Guide 49
7. Click OK to open the Data Dependent NL MS3 template ( Figure 37 ).
Figure 37. Data Dependent NL MS3 template
8. Enter the scan event settings by clicking the Scan Event 1 , Scan Event 2 , and
Scan Event 3 bars.
For Scan Event 2 and Scan Event 3, the Dependent Scan check box (lower left corner)
and its adjacent Settings button become active.
9. (For Scan Event 2 and Scan Event 3 only) Click Settings to open the Data Dependent
Settings dialog box ( Figure 33 on page 46 ).
10. In the left pane, under the Scan Event list, click Activation to see the activation
parameters ( Figure 34 on page 46 ).
The activation type is automatically set to ETD.
11. In the Default Charge State box, enter a value of 5 or more.