Thermo Scientific Dionex ICS-5000, Operator'S Manual

Page: 195 / 482

5 • Operation
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to the sample port on the injection valve. If not, refer to Section 9.20 for
connection instructions.
2. Fill a syringe with a calibration standard or sample.
3. Insert the syringe into the sample loading port on the front of the DC.
4. Verify that the injection valve is in the Load position. If it is not, switch
the valve by clicking the valve control on the Chromeleon 7 ePanel Set or
the Chromeleon 6.8 panel tabset, or by pressing the
VALVE
button on the
front of the DC.
5. Overfill the sample loop with several sample loop volumes. Excess
sample will exit through the injection valve waste line.
6. Leave the syringe in the port. This prevents the sample from exiting the
loop before injection.
7. Switch the injection valve to the Inject position.
5.3.3 Loading Samples with a Vacuum Syringe (Pull Method)
1. Verify that a luer adapter fitting is installed on the sample loading port on
the front of the DC and that tubing is connecting the sample loading port
and the injection valve. If this is not the case, refer to
Section 9.20 for
connection instructions.
2. Disconnect the waste line from the injection valve and replace it with a 25
to 30 cm (10 to 12 in) piece of PEEK or PTFE tubing (see Figure 5-8 ).
3. Place the free end of the line into the sample.
4. Verify that the injection valve is in the Load position. If it is not, switch
the valve by clicking the valve control on the Chromeleon 7 ePanel Set or
the Chromeleon 6.8 panel tabset, or by pressing the
VALVE
button on the
front of the DC.
5. Insert a 5 cc syringe into the sample loading port on the DC front door
(see Figure 5-8 ) and pull out the plunger to draw the sample into the
injection valve.