Thermo Scientific Dionex ICS-5000, Operator'S Manual

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Dionex ICS-5000 Operator’s Manual
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Doc. 065342-06 12/11
5.3 Loading Samples Into the Sample Loop or
Concentrator
Two methods are available for loading samples:
•
Loading samples with an autosampler (see Section 5.3.1 )
•
Loading samples with a syringe through the sample loading ports on the
DC front door (see
Section 5.3.2 )
5.3.1 Loading Samples with an Autosampler
1. Verify that the autosampler output line is connected to the sample port on
the DC (or TC) injection valve. Direct the waste line as required for this
autosampler model.
2. Prepare and fill the sample vials and place them in the autosampler tray or
carousel. Refer to the autosampler manual for detailed instructions.
Autosampler manuals are provided on the Thermo Scientific Reference
Library DVD (P/N 053891).
3. Create a sequence in Chromeleon that specifies the vials from which to
take sample injections and the order in which the injections should be run.
4. Specify a Chromeleon 7 instrument method or Chromeleon 6.8 program
that includes the appropriate load and inject commands for the
autosampler. See the examples in
Section 5.4 .
5.3.2 Loading Samples with a Syringe
This section describes two methods for using a syringe to load sample into the
DC injection valve sample loop:
•
Loading sample with a syringe through the sample loading port on the
front of the DC (push method)
•
Loading sample with a vacuum syringe through the sample loading port
on the front of the DC (pull method)
Loading Samples with a Syringe (Push Method)
1. Verify that a luer adapter fitting is installed on the sample loading port on
the front of the DC and that tubing is connecting the sample loading port