Thermo Scientific Dionex AutoTrace 280, Operator'S Manual

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Dionex AutoTrace 280 Operator’s Manual
48
Doc. 065330-05 03/2020
3.3.2 Setup Parameters
Table 3-2 lists some considerations for setting method parameters.
3.3.3 Method Steps
Table 3-3 lists some considerations for designating the steps in a method.
If... Then...
You need to improve recovery Decrease the flow rate(s)
You need to improve throughput Increase the flow rate(s)
The liquid handling syringe stalls Decrease the flow rate(s)
You are using viscous liquids Adjust the Push Delay
You need to decrease the amount of air
between multiple conditioning steps
Reduce the Air Factor
a
a. When using an Air Factor of less than 1, always perform a Cartridge
Rinse (or Disk Rinse ) after loading the sample.
Table 3-2. Setup Parameters
If... Then...
The sample contains volatile compounds Add a Pause and Alert step
between the conditioning and
loading steps (to ensure that
samples are not introduced
before the system is ready)
The manual method requires a “rinse with
water” step without allowing the water to
leave the cartridge or disk Use a Condition Cartridge (or
Condition Disk ) step rather than
a Cartridge Rinse (or Disk
Rinse ) step, so that a smaller air
push is used
You need an extended contact time
between solvent and packing material Introduce a Timed Pause step
The sample contains a large amount of
solids Add 5% to 7% to your volume
entry for the
Load Sample step
–or–
Filter the sample, collect the
filtrate, and place this in the
sample container
Table 3-3. Method Step Considerations