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Thermo Scientific BIOShield 720, Instruction Manual

Introducing the Thermo Scientific BIOShield 720, a cutting-edge laboratory instrument designed for robust biosafety containment. Unlock the full potential of this device with our comprehensive Instruction Manual. Download it for free from manualshive.com and harness the power of knowledge to enhance your research and experiment with confidence.

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Thermo Scientific

75003621 BIOShield

TM

 720

B-1

B

Chemical Compatibility Chart 

CHEMICAL

MA

TER

IA

L

ALUMIN

I

UM

ANODIC COATING for

 AL

UMIN

I

UM

BU

NA

 N

CE

LL

UL

OSE A

C

ET

A

TE

 BUTYRA

TE

POL

YURETH

ANE ROTO

R P

A

INT

CO

MPOSIT

E Car

bon

 Fi

ber

/Epo

xy

DEL

RI

N

®

ETHYL

ENE P

R

O

PYLE

N

E

GLA

S

S

N

EOPRENE

NO

RY

L

®

NY

LO

N

PE

T

*

, POL

YCLEAR

®

,C

LE

AR

CR

IM

P

®

C

CCLEARCR

IMP

®

POL

YA

LL

O

ME

R

POL

YCA

R

BONA

TE

POL

YESTER, G

LA

S

S TH

ERMOSET

POL

YTHE

RM

IDE

POL

YR

THYL

ENE

POL

YPROPYL

ENE

POL

YSU

LF

O

NE

POL

YV

IN

YL CHLORIDE

RU

LON

 A

®

, TE

FL

ON

®

SI

LI

C

O

N

E RU

BB

ER

S

TAI

N

LES

S ST

EE

L

TIT

A

NIU

M

TYGON

®

VI

TO

N

®

2-mercaptoethanol

S

S

U -

S

M S

-

S

U S

S

U S

S

-

S

S

S

S

U S

S

S

S

S

S

Acetaldehyde

S

-

U U -

-

-

M -

U -

-

-

M U U U M M -

M S

U -

S

-

U

Acetone

M S

U U S

U M S

S

U U S

U S

U U U S

S

U U S

M M S

U U

Acetonitrile

S

S

U -

S

M S

-

S

S

U S

U M U U -

S

M U U S

S

S

S

U U

Alconox

®

U U S

-

S

S

S

-

S

S

S

S

S

S

M S

S

S

S

S

S

S

S

S

S

S

U

Allyl Alcohol

-

-

-

U -

-

S

-

-

-

-

S

-

S

S

M S

S

S

-

M S

-

-

S

-

-

Aluminum Chloride

U U S

S

S

S

U S

S

S

S

M S

S

S

S

-

S

S

S

S

S

M U U S

S

Formic Acid (100

 

%)

-

S

M U -

-

U -

-

-

-

U -

S

M U U S

S

-

U S

-

U S

-

U

Ammonium Acetate

S

S

U -

S

S

S

-

S

S

S

S

S

S

S

U -

S

S

S

S

S

S

S

S

S

S

Ammonium Carbonate

M S

U S

S

S

S

S

S

S

S

S

S

S

U U -

S

S

S

S

S

S

M S

S

S

Ammonium Hydroxide (10

 

%)

U U S

U S

S

M S

S

S

S

S

-

S

U M S

S

S

S

S

S

S

S

S

M S

Ammonium Hydroxide (28

 

%)

U U S

U S

U M S

S

S

S

S

U S

U M S

S

S

S

S

S

S

S

S

M S

Ammonium Hydroxide (conc.)

U U U U S

U M S

-

S

-

S

U S

U U S

S

S

-

M S

S

S

S

-

U

Ammonium Phosphate

U -

S

-

S

S

S

S

S

S

S

S

-

S

S

M -

S

S

S

S

S

S

M S

S

S

Ammonium Sulfate

U M S

-

S

S

U S

S

S

S

S

S

S

S

S

-

S

S

S

S

S

S

U S

S

U

Amyl Alcohol

S

-

M U -

-

S

S

-

M -

S

-

M S

S

S

S

M -

-

-

U -

S

-

M

Aniline

S

S

U U S

U S

M S

U U U U U U U -

S

M U U S

S

S

S

U S

Sodium Hydroxide (<1

 

%)

U -

M S

S

S

-

-

S

M S

S

-

S

M M S

S

S

S

S

S

M S

S

-

U

Sodium Hydroxide (10

 

%)

U -

M U -

-

U -

M M S

S

U S

U U S

S

S

S

S

S

M S

S

-

U

Barium Salts

M U S

-

S

S

S

S

S

S

S

S

S

S

S

M -

S

S

S

S

S

S

M S

S

S

Benzene

S

S

U U S

U M U S

U U S

U U U M U M U U U S

U U S

U S

Benzyl Alcohol

S

-

U U -

-

M M -

M -

S

U U U U U U U -

M S

M -

S

-

S

Boric Acid

U S

S

M S

S

U S

S

S

S

S

S

S

S

S

U S

S

S

S

S

S

S

S

S

S

Summary of Contents for BIOShield 720

Page 1: ...Visit us online to register your warranty thermofisher com labwarranty Thermo Scientific BIOShield 720 Instruction Manual 50121034 d 07 2020 ...

Page 2: ...WEEE Conformity This product is subject to the regulations of the EU Waste Electrical Electronic Equipment WEEE Directive 2012 19 EU It is marked by the following symbol ...

Page 3: ......

Page 4: ......

Page 5: ...ding 4 1 Before a Run 4 2 Proper Loading 4 2 Improper Loading 4 3 Maximum Loading 4 3 Cycle Counter 4 4 Chapter 5 Aerosol tight Applications 5 1 Basic Principles 5 2 Placing O ring 5 2 Fill Level 5 2 Checking the Aerosol Tightness 5 2 Table of Contents Chapter 6 Maintenance and Care 6 1 Cleaning intervals 6 2 Cleaning 6 2 Disinfection 6 3 Decontamination 6 4 Autoclaving 6 5 Service of Thermo Fishe...

Page 6: ......

Page 7: ...t in the expiration of the seller s warranty Scope of Supply If any parts are missing please contact the nearest Thermo Fisher Scientific representative Precautions In order to ensure safe operation of the BIOShield 720 the following general safety regulations must be followed Do not remove the magnet at the rotor bottom Do not use rotors which show any signs of corrosion and or cracks Use only wi...

Page 8: ... pay particular attention to the following aspects Rotor installation Check that the rotor is locked properly into place before operating the centrifuge Always balance the samples Maximum sample density at maximum speed 1 2 g cm 3 This symbol refers to general hazards ATTENTION means that material damage could occur WARNING means that injuries or material damage or contamination could occur This s...

Page 9: ...Thermo Scientific 75003621 BIOShieldTM 720 1 1 1 Rotor Specifications Contents Technical Data on page 1 2 ...

Page 10: ...mple heating at nmax C referred to ambient temperature of 23 C running time 60 minutes 16 9 Aerosol tight Tested by HPA Porton down UK yes yes Permissible temperature range autoclavable C 121 121 Table 1 2 230 V 50 60 Hz refrigerated Centrifuge Multifuge X1R Megafuge 16R Catalog 75004250 75004270 Weight of empty rotor kg 5 7 5 7 Max cycle number 66 000 66 000 Maximum permissible load g 4 x 470 4 x...

Page 11: ...ating at nmax C referred to ambient temperature of 23 C running time 60 minutes 16 9 Aerosol tight Tested by HPA Porton down UK yes yes Permissible temperature range autoclavable C 121 121 Table 1 4 120 V 60 Hz refrigerated Centrifuge Multifuge X1R Megafuge 16R Catalog 75004251 75004271 Weight of empty rotor kg 5 7 5 7 Max cycle number 66 000 66 000 Maximum permissible load g 4 x 470 4 x 470 Maxim...

Page 12: ...ating at nmax C referred to ambient temperature of 23 C running time 60 minutes 16 9 Aerosol tight Tested by HPA Porton down UK yes yes Permissible temperature range autoclavable C 121 121 Table 1 6 230 V 50 60 Hz refrigerated Centrifuge Sorvall Legend X1R Sorvall ST 16R Catalog 75004260 75004380 Weight of empty rotor kg 5 7 5 7 Max cycle number 66 000 66 000 Maximum permissible load g 4 x 470 4 x...

Page 13: ...ating at nmax C referred to ambient temperature of 23 C running time 60 minutes 16 9 Aerosol tight Tested by HPA Porton down UK yes yes Permissible temperature range autoclavable C 121 121 Table 1 8 120 V 60 Hz refrigerated Centrifuge Sorvall Legend X1R Sorvall ST 16R Catalog 75004261 75004381 Weight of empty rotor kg 5 7 5 7 Max cycle number 66 000 66 000 Maximum permissible load g 4 x 470 4 x 47...

Page 14: ...ting at nmax C referred to ambient temperature of 23 C running time 60 minutes 16 9 Aerosol tight Tested by HPA Porton down UK yes yes Permissible temperature range autoclavable C 121 121 Table 1 10 100 V 50 60 Hz refrigerated Centrifuge Sorvall Legend X1R Sorvall ST 16R Catalog 75004263 75004283 Weight of empty rotor kg 5 7 5 7 Max cycle number 66 000 66 000 Maximum permissible load g 4 x 470 4 x...

Page 15: ... Sample heating at nmax C referred to ambient temperature of 23 C running time 60 minutes 9 Aerosol tight Tested by HPA Porton down UK yes Permissible temperature range autoclavable C 121 Table 1 12 230 V 50 60 Hz refrigerated Centrifuge Thermo Scientific SL 16R Catalog 75004030 Weight of empty rotor kg 5 7 Max cycle number 66 000 Maximum permissible load g 4 x 470 Maximum speed nmax rpm 5 300 Max...

Page 16: ... Sample heating at nmax C referred to ambient temperature of 23 C running time 60 minutes 9 Aerosol tight Tested by HPA Porton down UK yes Permissible temperature range autoclavable C 121 Table 1 14 120 V 60 Hz refrigerated Centrifuge Thermo Scientific SL 16R Catalog 75004031 Weight of empty rotor kg 5 7 Max cycle number 66 000 Maximum permissible load g 4 x 470 Maximum speed nmax rpm 5 300 Maximu...

Page 17: ...Thermo Scientific 75003621 BIOShieldTM 720 2 1 2 Accessories Contents Rotor data on page 2 2 Accessories on page 2 2 ...

Page 18: ...4959 50 50 Nunc Conical 25 6 300 29 5 x 116 Included 25 PP Sealing 75003677 2 2 50 Round Bottom Open Top Tube 34 x 120 75003816 2 2 25 Universal 22 5 x 105 75003818 2 4 25 DIN Round Bottom Tube 25 x 107 75003817 2 5 366036 15 15 Nunc Conical 50 6 300 16 5 x 121 Included 50 PP Sealing 75003678 2 6 15 Blood Collection 17 x 109 75003820 2 11 10 Blood Collection 17 x 109 75003820 2 11 7 Blood Collecti...

Page 19: ...Thermo Scientific 75003621 BIOShieldTM 720 3 1 3 AutoLock Contents Rotor Installation on page 3 2 Removing the Rotor on page 3 3 ...

Page 20: ...fuge spindle The rotor clicks automatically into place 3 Check if the rotor is properly installed by lifting slightly on the handle If the rotor can be pulled up then it must be reclamped to the centrifuge spindle CAUTION Unapproved or incorrectly combined accessories can cause serious damage to the centrifuge CAUTION Do not force the rotor onto the centrifuge spindle If the rotor is very light th...

Page 21: ... handle with both hands and press against the green AutoLock button At the same time pull the rotor directly upwards with both hands and remove it from the centrifuge spindle Make sure not to jam the rotor while doing this CAUTION Check that the rotor is properly locked on the centrifuge spindle before each use by pulling its handle Be sure to check all seals before starting any aerosol tight appl...

Page 22: ......

Page 23: ...ermo Scientific 75003621 BIOShieldTM 720 4 1 4 Rotor Loading Contents Before a Run on page 4 2 Proper Loading on page 4 2 Improper Loading on page 4 3 Maximum Loading on page 4 3 Cycle Counter on page 4 4 ...

Page 24: ...mpatibility chart on page B 1 5 Make sure the tubes or bottles do not touch the bucket lid 6 Check the rotor bolts and apply grease 75003786 before using for the first time 7 Check that each bucket or microplate carrier can swing freely by moving it carefully with your hand Weigh the bucket content adapter and tube Make sure you do not exceed the maximum compartment load Proper Loading To ensure s...

Page 25: ... The safety system of the centrifuge requires that you do not overload the rotor There are two options available for centrifuging samples whose weight including adapter exceeds the maximum permissible load Reduce the fill level Reduce the speed Use the table or the formula Actual Load g bucket RPM max 470 6300 490 6170 510 6048 530 5933 550 5824 570 5721 590 5623 610 5530 630 5442 650 5357 670 527...

Page 26: ...les is given in the rotor table in the rotor specification section The maximum number of cycles for buckets is marked on the buckets themselves Service life examples 750 4987 770 4922 Actual Load g bucket RPM max nadm nmax Maximum permissible load Effective load WARNING Replace the rotor when the specified number of cycles is reached Due to the mechanical load a rotor can break and thus damage the...

Page 27: ...Thermo Scientific 75003621 BIOShieldTM 720 5 1 5 Aerosol tight Applications Contents Basic Principles on page 5 2 Fill Level on page 5 2 Checking the Aerosol Tightness on page 5 2 ...

Page 28: ...e during centrifugation Therefore fill the tube only 2 3 of the rated level Checking the Aerosol Tightness The aerosol tightness testing of the rotors and buckets depend on the microbiological test process in accordance with the EN 61010 2 020 Appendix AA Whether or not a rotor is aerosol tight depends primarily on proper handling Check as needed to make sure your rotor is aerosol tight The carefu...

Page 29: ... releases the carbonic acid gas which is bound in the water resulting in excess pressure Do not apply pressure to the lid when doing so Leaks can be detected by escaping water or the sound of escaping gas Replace the seals if you detect any leaks Then repeat the test 5 Dry the rotor rotor cover and the cover seal CAUTION Prior to each use the seals in the bucket are to be inspected in order to ass...

Page 30: ......

Page 31: ...BIOShieldTM 720 6 1 6 Maintenance and Care Contents Cleaning intervals on page 6 2 Cleaning on page 6 2 Disinfection on page 6 3 Decontamination on page 6 4 Autoclaving on page 6 5 Service of Thermo Fisher Scientific on page 6 6 ...

Page 32: ...the parts Use only disinfectants with a pH of 6 8 Dry aluminium parts off with a soft cloth After cleaning treat the entire surface of aluminium parts with corrosion protection oil 70009824 Also treat the cavities with oil Store the aluminium parts at room temperature or in a cold storage room with the cavities pointing down Maintenance Recommended interval Clean rotor chamber Daily or when pollut...

Page 33: ...ctious material has spilled during centrifugation Use a sprayer whenever possible so that all surfaces are covered evenly The rotor chamber and the rotor should be treated preferably with a neutral disinfectant A disinfectant spray would be most suitable for this purpose so that the rotor and accessory surfaces are covered evenly CAUTION When cleaning do not allow liquids especially organic solven...

Page 34: ... in a warm air cabinet at a maximum temperature of 50 C 11 After cleaning treat the entire surface of aluminium parts with corrosion protection oil 70009824 Also treat the cavities with oil 12 Treat the bolt of the swing out rotor with bolt grease 75003786 Decontamination Decontaminate the centrifuge immediately whenever radioactive material has spilled during centrifugation For general radioactiv...

Page 35: ...with a cloth or in a warm air cabinet at a maximum temperature of 50 C 11 After cleaning treat the entire surface of aluminium parts with corrosion protection oil 70009824 Also treat the cavities with oil 12 Treat the bolt of the swing out rotor with bolt grease 75003786 Autoclaving 1 Before autoclaving clean the rotor and accessories as described above 2 Place the rotor on a flat surface Rotors a...

Page 36: ...mends having the centrifuge and accessories serviced once a year by an authorized service technician The service technicians check the following the electrical equipment the suitability of the set up site the lid lock and the safety system the rotor the fixation of the rotor and the drive shaft Thermo Fisher Scientific offers inspection and service contracts for this work ...

Page 37: ... 181 1100 6 7 16 2 91 219 1200 6 7 16 2 108 261 1300 6 7 16 2 127 306 1400 6 7 16 2 147 355 1500 6 7 16 2 169 408 1600 6 7 16 2 192 464 1700 6 7 16 2 216 523 1800 6 7 16 2 243 587 1900 6 7 16 2 270 654 2000 6 7 16 2 300 724 2100 6 7 16 2 330 799 2200 6 7 16 2 363 877 2300 6 7 16 2 396 958 2400 6 7 16 2 431 1043 2500 6 7 16 2 468 1132 2600 6 7 16 2 506 1224 2700 6 7 16 2 546 1320 2800 6 7 16 2 587 ...

Page 38: ... 4300 6 7 16 2 1385 3349 4400 6 7 16 2 1450 3506 4500 6 7 16 2 1517 3668 4600 6 7 16 2 1585 3832 4700 6 7 16 2 1655 4001 4800 6 7 16 2 1726 4173 4900 6 7 16 2 1798 4349 5000 6 7 16 2 1873 4528 5100 6 7 16 2 1948 4711 5200 6 7 16 2 2025 4897 5300 6 7 16 2 2104 5088 5400 6 7 16 2 2184 5281 5500 6 7 16 2 2266 5479 5600 6 7 16 2 2349 5680 5700 6 7 16 2 2434 5884 5800 6 7 16 2 2520 6093 5900 6 7 16 2 2...

Page 39: ...S S S S U S S S S M S S S S S S S S S M U U S S Formic Acid 100 S M U U U S M U U S S U S U S U Ammonium Acetate S S U S S S S S S S S S S U S S S S S S S S S S Ammonium Carbonate M S U S S S S S S S S S S S U U S S S S S S M S S S Ammonium Hydroxide 10 U U S U S S M S S S S S S U M S S S S S S S S S M S Ammonium Hydroxide 28 U U S U S U M S S S S S U S U M S S S S S S S S S M S Ammonium Hydroxide...

Page 40: ...S S U S U S U S S S M S S S S S S Dimethylsulfoxide S S U U S S S S U S S U S U U S S U U S S S S U U Dioxane M S U U S S M M S U U S U M U U M M M U S S S S U U Ferric Chloride U U S M S M S S S S M U S S Acetic Acid Glacial S S U U S S U M S U S U U U U U M S U M U S U U S U Acetic Acid 5 S S M S S S M S S S S S M S S S S S S S M S S M S S M Acetic Acid 60 S S U U S S U S M S U U M U S M S M S M...

Page 41: ...Hydroxide conc U U M U M M S S U M U U U S M M U U U U Potassium Permanganate S S S S S S S S S U S S S M S M S U S S M S U S Calcium Chloride M U S S S S S S S S S S S S M S S S S S S S M S S S Calcium Hypochlorite M U S M M S M S S M S S S S M S M U S S Kerosene S S S S S S U S M U S U M M S M M M S S U S S U S Sodium Chloride 10 S S S S S S S S S S S S S S S S S S M S Sodium Chloride sat d U S ...

Page 42: ... S S S S S M S S S S S S M S S S S S S S S S S Nickel Salts U S S S S S S S S S S S S S S S S S S M S S S Oils Petroleum S S S S U S S S S U U M S M U U S S S U S S S S Oils Other S S S M S S S S U S S S S U S S S S S S M S Oleic Acid S U S S S U U S U S S M S S S S S S S S S M U S M M Oxalic Acid U U M S S S U S S S S S U S U S S S S S S S S U M S S Perchloric Acid 10 U U S U U S M M M U M S M M ...

Page 43: ...U U Toluene S S U U S S M U S U U S U U U S U M U U U S U S U U M Trichloroacetic Acid U U U S S U M S U S U U S M M S S U U S U U U M U Trichloroethane S U M U U S U U U U U U U U U S U S S Trichloroethylene U U U U S U U U U U U U U U S U U S Trisodium Phosphate S M S S S S S S S Tris Buffer neutral pH U S S S S S S S S S S S S S S S S S S S S S S S S S Triton X 100 S S S S S S S S S S S S S S S...

Page 44: ...sts for materials under the stress of centrifugation when in doubt we recommend pretesting sample lots Key S Satisfactory M Moderate attack may be satisfactory for use in centrifuge depending on length of exposure speed involved etc suggest testing under actual conditions of use U Unsatisfactory not recommended Performance unknown suggest testing using sample to avoid loss of valuable material ...

Page 45: ...ty Chart B 1 Cleaning 6 2 Cleaning Agents 6 3 Cycle Counter 4 4 D Decontamination 6 4 Disinfection 6 3 F Fill Level 5 2 I Improper Loading 4 3 M Maintenance 6 1 Maximum Loading 4 3 P Placing O ring 5 2 Precautions iii Preface iii Proper Loading 4 2 Q Quick Test 5 3 R RCF Values A 1 Removing the Rotor 3 3 Rotor Installation 3 2 Rotor Loading 4 1 Rotor Specifications 1 1 S Scope of Supply iii Servic...

Page 46: ......

Page 47: ...s terms and pricing are subject to change Not all products are available in all countries Please consult your local sales representative for details Shown pictures within the manual are examples and may differ considering the set parameters and language Pictures of the user interface within the manual are showing the English version as example Australia 61 39757 4300 Austria 43 1 801 40 0 Belgium ...

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