Thermo Scientific Applied Biosystems SeqStudio Genetic Analyzer, User Manual

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Run modules
Run modules, read lengths, size ranges, and run times
Table 15 Sequencing run modules for standard sequencing
Run module
Contiguous read
length (CRL)
[1]
QV threshold
Approximate run
time
ShortSeq
≥
350 QV30 30 minutes
ShortSeq_BDX
MediumSeq
≥
500 QV30 45 minutes
MediumSeq_BDX
LongSeq
≥
800 QV20 ~ 2 hours
LongSeq_BDX
[1]
CRL was determined using the Long Read Sequencing standard. A minimum of 90% of analyzed sequences with an average QV ≥QV threshold
were observed.
IMPORTANT! Use BDX run modules only if you prepare samples with BigDye
XTerminator
™
Purification Kit. Use non-BDX run modules for samples purified with
other methods.
Table 16 Fragment analysis run modules
Run module
Resolution
range
Approximate
run time
Sizing precision
Compatible size
standards
SNaPshot 40–120 bp 25 minutes 40–120: <0.5 GeneScan ™ 120 LIZ ™ Size
Standard
FragAnalysis 60–460 bp
[1]
45 minutes 60–460: <0.15 All except GeneScan ™
1200 LIZ
™
Size Standard
LongFragAnalysis
[2]
60–600 bp
[1]
< 2 hours 60–460: <0.15
461–600: <0.3
601–800: >0.45
• GeneScan ™ 600 LIZ ™
Size Standard v2.0
• GeneScan ™ 1200
LIZ ™ Size Standard
[1]
Resolution Range: The range of bases over which the resolution (peak spacing interval divided by the peak width at half-max in a GS600 or
GS1200 LIZ size standard sample sized with a third order fit) is
≥
1. The table shows the resolution range in
≥
90% of samples.
[2]
Load a maximum of 48 samples per plate if you use a long run module.
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218 SeqStudio
™
Genetic Analyzer Instrument and Software User Guide