Prepare the digital PCR reaction and
load the chips
■
Guidelines for loading and sealing chips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
■
Prepare the DNA samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
■
Prepare the digital PCR reactions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
■
Load the chips using the QuantStudio
3D Digital PCR Chip Loader . . . . . . 24
Guidelines for loading and sealing chips
To ensure proper processing and analysis of loaded chips, handle them according to
the following guidelines:
• Always wear powder-free gloves when loading and sealing chips.
IMPORTANT!
Never handle chips or chip lids without gloves. Oils from your
hands can contaminate the components and interfere with thermal cycling and
imaging.
• Hold the chip and lid gently by their sides.
– If you accidentally touch the chip surface, discard the chip.
– If you accidentally touch the chip lid window, clean it using a laboratory wipe
that has been sprayed with isopropanol, then dry by wiping in one direction
using a clean low-lint wipe.
443MPJ
• Use a permanent pen or marker to label the back of each loaded chip for sample
tracking.
• Load each chip within 2 hours after opening it.
• Load chips in alphanumeric order (according to the chip ID printed on the lid) to
avoid data entry errors.
3
QuantStudio
™
3D Digital PCR System User Guide
19
