Thermo Scientific Accela UV/Vis Plus, Hardware Manual

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2
Installation
Installing the LightPipe Flow Cell
Thermo Scientific Accela UV/Vis Detector Hardware Manual 15
2. Using a one-piece PEEK fitting, connect the (red) insulated, 0.005 in. ID tubing
(supplied) to the inlet port of the 10 mm or 50 mm LightPipe flow cell (see Figure 14 or
Figure 15 ). Tighten the fitting with a 1/4 in. open-end wrench.
Figure 14. 10 mm LightPipe flow cell tubing connections
Figure 15. 50 mm LightPipe flow cell tubing connections
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3. Using a fingertight fitting, connect the other end of the insulated, 0.005 in. ID PEEK
inlet tubing to the LC column outlet.
Note The insulating sleeve of the inlet tubing minimizes temperature fluctuations,
which cause baseline drift.
Note The 10 mm LightPipe flow cell is the same length as the 50 mm LightPipe flow
cell so that it fits into the retaining block without the need for an adapter.
IMPORTANT If you have several detectors (fluorescence, refractive index,
electrochemical, and so on) hooked up in a series, place your UV/Vis detector closest
to the column outlet. The LightPipe flow cell in the UV/Vis detector can withstand a
higher backpressure than the other flow cells.
Tip For best results, when you run the system at a low backpressure, use a
backpressure regulator to prevent bubble formation in the detector’s LightPipe
flow cell.
Outlet port Inlet port
Inlet port Outlet port