2
Accela 600 Pump or Accela 1250 Pump Installation
Solvent Line Connections
30
Accela 600 and 1250 Pump Hardware Manual
Thermo Scientific
Priming the Degassing Membranes
Prime the degassing membranes as part of the initial Accela pump installation procedure.
To prime the degassing membranes
1. Remove the tubing connected to inlet valve A.
2. Connect a 10 mL syringe to the end of the disconnected tubing.
3. Draw approximately 5 mL solvent into the syringe.
4. Disconnect the syringe and reconnect the tubing to inlet valve A.
5. Repeat
through
to prime solvents B, C, and D through the degasser if
applicable.
High-Pressure Solvent Line Between the Pump and the Autosampler
To connect the high-pressure solvent line between the pump and the autosampler, follow
these procedures:
•
Connecting the High-Pressure Solvent Line to the Pump Outlet
•
Connecting the High-Pressure Solvent Line to the Autosampler
CAUTION
To avoid damaging the degassing unit and the piston seals, prime the degassing
membranes by using a syringe to pull solvent through the solvent lines into the degassing
unit.
Do not push the solvent into the degasser. Pushing the solvent can generate excessive
pressure and rupture the degassing membranes. The maximum recommended pressure on
the membrane is 10 bar (145 psi).
Do not use the pump to prime the solvent lines and the degassing membranes. Drawing
excessive amounts of air through the liquid displacement assembly can damage the piston
seals.
IMPORTANT
Avoid using PEEK tubing and fittings with system pressures above
276 bar (4000 psi). Thermo Fisher Scientific ships precut 1/16 in. OD, stainless steel
tubing and two-piece, stainless steel compression fittings with the Accela autosamplers.
CAUTION
To prevent personal injury caused by skin contact with hazardous solvents,
turn off the pump flow before you connect the high-pressure solvent line between the LC
pump and the autosampler.
