4.
Install the two buckets. Position the buckets with the larger semi-circular cut-outs
facing out, and ensure that the buckets hang freely.
1
1
Correct orientation
2
Incorrect orientation
2
1.
Prepare two previously-used chips:
a.
Inject 100 µL of isopropanol two times into the loading port of each chip.
After each injection, remove the expelled liquid from the opposite port.
Note:
Use 50 µL volume of isopropanol if testing Ion PGM
™
sequencing
chips.
b.
Aspirate the remaining isopropanol from the flow cells for 5–10 seconds.
Confirm that the chips are dry.
Note:
To aspirate the isopropanol, attach a P200 pipette tip to a vacuum
line, then place the pipette tip in the chip loading port.
Test the minifuge
Appendix C
Supplemental procedures
Set up and test the Ion Chip
™
Minifuge
C
50
Ion GeneStudio S5 Instrument User Guide
