Terumo BCT Quantum Cell Expansion System, Operator'S Manual

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Release Adherent Cells And Harvest
The purpose of this task is release and harvest cells. This task begins with the Release Adherent Cells task
followed by the Harvest Cells task. This task includes five separate steps:
•
Step 1: performs the IC EC Washout task in preparation for adding a reagent. For example, the
system replaces IC media and EC media with PBS to remove protein, Ca++, and Mg++ in
preparation for adding trypsin.
• Step 2: loads a reagent into the system until the bag is empty.
• Step 3: chases the reagent into the IC circulation loop.
• Step 4: mixes the reagent within the IC circulation loop.
• Step 5: transfers the cells in suspension from the IC circulation loop, including cells in the
bioreactor, to the harvest bag.
Table 12-6 describes the types of solution that are needed to attach to each line when performing Release
Adherent Cells And Harvest. These solutions and corresponding volumes are based on the factory
default settings for this task.
As a precondition before starting this task:
Fill the cell inlet bag with a minimum of approximately 40 mL of air.
Note: When harvesting cells, you should adjust the media composition in the harvest bag to ensure that it is
appropriate for the way in which you intend to use the cells after harvest.
Table 12-6: Solutions for Release Adherent Cells And Harvest
Line
Solution
Attached to the
Line
Required Volume (estimate based on factory default settings)
Cell None N/A
Reagent Trypsin As desired
IC Media Harvest media 0.4 L
Wash PBS 1.5 L
EC Media Media without
protein 0.1 L
Release Adherent Cells And Harvest pathway:
Task
>
Release and Harvest
>
Release Adherent Cells And
Harvest
Release Adherent Cells And Harvest
Quantum
®
Cell Expansion System • Operator's Manual for Software Version 2.1 12-5