Terumo BCT Quantum Cell Expansion System, Operator'S Manual

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Feed Cells With Ultrafiltration
The purpose of this task is to add fluid at a low flow rate to the IC circulation loop and/or the
EC circulation loop. If you use the factory default settings for this task and the solution volumes based on
those defaults, large molecules could become concentrated in the IC circulation loop. In this instance,
the task would use ultrafiltration to remove excess fluid from the IC side of the bioreactor.
Table 11-1 describes the types of solution that are needed to attach to each line when performing Feed
Cells With Ultrafiltration. These solutions and corresponding volumes are based on the factory default
settings for this task.
Table 11-1: Solutions for Feed Cells With Ultrafiltration
Line
Solution Attached
to the Line
Required Volume (estimate based on factory default
settings)
Cell None N/A
Reagent None N/A
IC Media Media with protein 6 mL/hour
Wash None N/A
EC Media None N/A
Feed Cells With Ultrafiltration pathway:
Task
>
Feed and Add
>
Feed Cells With Ultrafiltration
1. Confirm the settings for Feed Cells With Ultrafiltration shown in Table 11-2.
Table 11-2: Settings for Feed Cells With Ultrafiltration
Setting Factory Default Setting Options
IC Inlet IC Media Cell, Reagent, IC Media, Wash, EC Media, None
IC Inlet Rate 0.1 mL/min 0 to 10 mL/min
IC Circulation Rate 20 mL/min −100 to 100 mL/min
The system automatically calculates this rate based on the IC Inlet Rate
for this task.
EC Inlet None Reagent, IC Media, Wash, EC Media, None
EC Inlet Rate 0 mL/min 0 to 10 mL/min
EC Circulation Rate 30 mL/min 10 to 300 mL/min
Outlet EC Outlet N/A
Rocker Stationary (0°) In Motion (−180° to 270°, 0 to 3600 sec), Stationary
(−180° to 270°)
Feed and Add Tasks
11-2 Quantum
®
Cell Expansion System • Operator's Manual for Software Version 2.1