Terumo BCT Quantum Cell Expansion System, Operator'S Manual

Page: 136 / 204

Attach Cells
The purpose of this task is to allow adherent cells to attach to the bioreactor membrane while allowing
flow in the EC circulation loop. The pump flow rate to the IC circulation loop is set to zero.
Table 10-8 describes the types of solution that are needed to attach to each line when performing Attach
Cells. These solutions and corresponding volumes are based on the factory default settings for this task.
Table 10-8: Solutions for Attach Cells
Line
Solution Attached to the
Line
Required Volume (estimate based on factory
default settings)
Cell None N/A
Reagent None N/A
IC Media None N/A
Wash None N/A
EC Media Media without protein 6 mL/hour
Attach Cells pathway:
Task
>
Load and Attach
>
Attach Cells
1. Confirm the settings for Attach Cells shown in Table 10-9.
Table 10-9: Settings for Attach Cells
Setting Factory Default Setting Options
IC Inlet None N/A
IC Inlet Rate 0 mL/min N/A
IC Circulation Rate 0 mL/min N/A
EC Inlet EC Media Reagent, IC Media, Wash, EC Media
EC Inlet Rate 0.1 mL/min 0.1 to 10 mL/min
EC Circulation Rate 30 mL/min 0 to 100 mL/min
Outlet EC Outlet N/A
Rocker Stationary (0°) Stationary (0° to 180°)
Stop Condition Manual Time (0.1 to 2880 min), Manual, EC Volume
(1 to 4000 mL)
2. Touch
Start
.
3. Check the status line to confirm that the task has started.
4. Touch
Finish
when you are ready to complete the task.
Attach Cells
Quantum
®
Cell Expansion System • Operator's Manual for Software Version 2.1 10-7