5. Optical System
90
Instructions for Use for
infinite® 200
No. 30017581 Rev. No. 1.4
2008-07
5.7.1
Luminescence Optics
In luminescence measurement mode, the
infinite
®
200
uses fixed microplate
position and a moveable luminescence measurement head (see
). The plate thickness is defined by selecting the
corresponding plate type in the software (see i-Control Instructions for Use).
Fiber
A glass fiber guides the light from the sample to the detection unit. The fiber is
designed to measure 96-well plates as well as 384-well plates.
Filter Wheel
A filter wheel with 6 filter positions in front of the PMT window is switched to the
required luminescence channel. The sensitivity of the detection system makes it
necessary to attenuate high luminescence light levels; therefore, the filter wheel
can also switch a neutral density filter across the selected fiber exit.
Filter Wheel Position
Filter
Position
1
Lumi
Green*
Position
2
Lumi
Magenta*
Position 3
1 OD neutral density filter
Position
4
No
attenuation
Position
5
Blue
1**
Position 6
Green 1**
*
recommended for BRET ™2 and ChromaGlo™-Luciferase Assay
**
recommended for BRET™ 1
to
Figure 5-17
for transmission spectra of luminescence filters.
0
10
20
30
40
50
60
70
80
90
100
400
450
500
550
600
650
Wavelength [nm]
T
ran
sm
issi
o
n
[
%
]
Figure 5-14: Transmission spectrum of filter ‘Lumi Magenta’
