7. Performance Testing/ Quality Control
2022-01
HYDROFLEX PLUS No. 30199906 Rev. No. 1.1
53
7. Performance Testing/
Quality Control
Using Gravimetrical Method
This chapter describes a QC-procedure for the HYDROFLEX PLUS allowing to
check the residual volume and dispense accuracy of the instrument by weighing
a Greiner-F 96-well plate (flat-bottom) on a calibrated lab balance.
Note
To ensure the proper performance of the HYDROFLEX PLUS with the
QC-test procedure described below, it is necessary to adjust the
instrument to the Greiner-F 96-well plate type prior to running this
procedure.
If this adjustment procedure is not performed properly, this might result
in high levels of residual volume left per well and can lead to failure of
the performance test.
For details on how to adjust the HYDROFLEX PLUS to the type of
microplate or strip-plate used, see chapter 6 Settings Menu of this
manual.
Necessary Tools
•
Calibrated lab-balance including cover for wind-protection,
capable of reading milligrams
•
Greiner F 96-well, flat-bottom, compact microplate
•
Small plastic syringe to aliquot Tween 20 solution
•
Clean 2.5-liter wash buffer bottle supplied with HYDROFLEX PLUS
•
5-liter waste bottle supplied with HYDROFLEX PLUS
Necessary Chemicals
•
1 liter of distilled water (alternatively deionized water can be used)
•
0.1% Tween 20 solution
Preparation of Solution for QC Procedure
•
Prepare a 0.1% Tween 20 solution
(1 liter of distilled or deionized water and 1 ml Tween 20).
•
Fill solution in empty 2.5-liter wash-buffer bottle supplied
with the HYDROFLEX PLUS, mix thoroughly and connect tubing to the
appropriate channel on the rear panel of the instrument.
Note
The solution for the QC-procedure can be stored for a maximum of
1 month under refrigeration. If the solution becomes turbid, it must be
disposed of and replaced with a fresh solution.
