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● This adjustment will enable the user to observe the specimen with both
eyes.
3. Diopter adjustment
● Diopter adjustment compensates for the differences in vision between
the left and right eyes. In addition to making observation through both
eyes easier, this adjustment also reduces the extent to which focusing is
lost when the objective magnification is changed. In particular, this occurs
when a low magnification objective is used.
● The left eyepiece tube has a diopter ring to compensate for slight
differences in the focusing of each eye.
● Set the diopter to “0” position and turn the nosepiece to 10x agnification.
● Using the right eye only and viewing through the right-hand eyepiece,
adjust the focus with the microscope coarse or fine adjustment until the
image of the specimen is at its sharpest.
● Using the left eye only and viewing through the left-hand eyepiece, turn
the diopter ring to correct focus for left eye until the specimen image is at
its sharpest.
● Change the magnification to higher magnification to verify the result and
if necessary repeat the procedure to match the sharpness for the higher
magnification.
● Keep this diopter position for all magnification/lenses. The diopter
position for each user can be recorded from the scale, so it can be easily
reset.
4. Coarse and fine focusing
● Focusing is carried out with the coarse and fine focus knobs at the left