6
NOTE: The tubing length supplied contains approximately 450 microliters of fluid. If a
syringe with less than 500 microliters volume is being used, a concomitantly shorter piece
of tubing must be attached, otherwise the fluid cannot be pushed to the tubing tip. A
guide to syringe volumes and appropriate tubing lengths is shown in the table in Figure
2-2, please refer to the Troubleshooting section for more information.
Syringe Volume Maximum Tubing
Length
1000
μ
l 1930 mm
500
μ
l 965 mm
100
μ
l 178 mm
Figure 2-2. Connecting the tubing to the tip of the syringe.
8.
Once the hydraulic fluid has been pushed to the very tip of the tubing, dip the tip of the
tubing into the bottle or dish of hydraulic fluid and very slowly draw more hydraulic fluid
into the tubing until the syringe is approximately ¾ full. Again, take particular care not
to cavitate the hydraulic fluid or draw up any air bubbles. It may be necessary to repeat
Steps 3 - 7 until all bubbles are purged from the syringe and tubing.
XENOWORKS ANALOG MICROINJECTOR OPERATION MANUAL – REV. 4.00 20100521
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Summary of Contents for XenoWorks BRI
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