17
STEINDORFF
®
4.2.
Reflected Light Simplified Polarized Light Observation
1.
Setting the Analyzer and Polarizer (Fig. 15).
2.
Place the specimen on the stage and adjust the focus by moving the stage up or down. Now simplified polarized light
Observation can be started.
3.
Adjust the field iris diaphragm so that its image circumscribes the field of view.
4.
The contrast may sometimes be enhanced by closing the aperture iris diaphragm slightly.
4.3.
Transmitted Light Observation
1.
Installing the lamp housing. (see page 8: Installing the lamp housing when using transmitted light illuminator)
2.
Installing objective.
3.
Installing the swing out condenser.
4.
Set the main switch to “II” (ON).
5.
Fix the specimen on the stage, adjust the focus to begin observation.
o
At the Transmitted Light Observation, the method of condenser, field iris diagram adjusting please refer to section
3.14 and 3.15.
4.4.
DIC Observation (Difference Interference Contrast Observation)
1.
Setting the Nomarski slider
Loosen the mounting knob
①
on the front of the DIC revolving nosepiece, insert the DIC slider
②
and clamp by
tightening the mounting knob.
2.
Observation method
2.1.
Place the specimen on the stage and adjust the focus by moving the stage up or down.
2.2.
Adjust the field iris diaphragm so that its image circumscribes in the field of view.
3.
Adjust the DIC attachment
3.1.
Adjust the background contrast by turning the slider
movement knob
③
on the DIC prism as described below.
3.2.
When the slider movement knob on the DIC prism is
turned, the interference color at the background varies
continuously from the gray sensitive color to magenta
sensitive color (from -100 to 600nm). Set the interference
color which can provide best contrast with respect to the
specimen.
Selecting a dark background color enables dark field-type observation.
Selecting a gray background color enables 3D-looking observation with high contrast thanks to the highest
sensibility of the gray sensitive color.
Selecting a magenta sensitive color allows even small phase variation to be observed as a change in color.
Since the DIC observation has a high detection sensitivity, take care against contamination on the specimen
surface.
②
①
③
