- 3 -
III. MEASUREMENT PROCEDURES
1. Gently pull upwards on the
ADJUSTABLE VOLUME WHEEL
and turn
it in either direction until the desired setting appears in the
DIGITAL
DISPLAY
(see chart below).
DIGITAL DISPLAY Settings
:
300100
300101
300102
300103
1
0
1
0
2
7
2
7
5
5
5
5
1.25µl
7.25µl
12.5µl
75µl
300104
300105
300106
1
0
0
2
7
2
5
5
5
125µl
0.75ML
2.5ML
2. To lock in your desired volume, push the
ADJUSTABLE VOLUME
WHEEL
back down into the locked position. The small grooves in the
bottom of the
WHEEL
align with the handle.
3. Secure a pipette tip to the
TIP
of the unit.
4. Holding the unit vertically, depress the
PLUNGER
to the first stop. Next
fully immerse the
TIP
2 to 4 mm into the solution.
5. Slowly release the
PLUNGER
until it returns to the original position.
6. Remove the unit from the solution after 1 to 2 seconds.
7. To discharge the solution from the pipette tip, place the
TIP
against the
inside wall of the receptacle. Depress the
PLUNGER
to the first stop,
then depress fully to completely blow out the solution from the
TIP
.
8. Next move the
TIP
up and along the inner wall of the receptacle several
times.
9. You may now remove the unit and release the
PLUNGER
.
10. Depress the
TIP EJECTOR
to remove the pipette tip.
11. Clean the unit using an autoclave or ultrasonic cleaner as required by
your application. The unit may be autoclaved for a maximum of 8 times,
after which, the unit should be discarded. Calibration remains stable
after occasional autoclaving. Annual recalibration by a professional
service center is recommended to ensure accuracy.
Notes:
•
Highly concentrated solutions may require increased or decreased
DIGITAL DISPLAY
settings. Make adjustments as needed.
•
Inserting objects into the top of the tip assembly can damage the pipette
seal.