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Sigma 3-18KS, Operating Manual

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Sigma 3-18KS Operating Manual Download Page 71

 

Refrigerated Centrifuge Sigma 3-18KS 

  

11 Appendix 

 

 

 

Version 08/2012, Rev. 1.15 of 15/09/2017 

• sb 

71 / 89 

Translation of the original operating manual, part no. 0701102 

 

Adapters and stainless steel tubes 

 

Part no. 

Description 

13000 

Adapter for reaction vials 0.25/0.4 ml no. 15014, suitable for 12110, 12131, 12154, 12349, 1 Set = 2 pcs. 

13002 

Adapter for reaction vials 0.5/0.75 ml, Ø 7.9/10 x 28/31 mm, e.g. no. 15005, suitable for 12110, 12131, 
12154, 12349, 1 set = 2 pcs. 

13021 

Adapter for PCR-tube 0.2 ml, 1 set = 2 pcs., suitable for 12110, 12131, 12154, 12349, 14002, 17008, 
18002

 

13055 

Stainless steel tube 50 ml, Ø 28.5 x 101.5 mm, sealable with cap no. 17054, suitable for 12150, 12156, 
13082 

13059 

Adapter for 1 round bottom tube 10 ml, max. Ø 16.2/19 x 75 - 85 mm, e.g. no. 15000, 15010, 15039, 
1 set = 2 pcs. 

13060 

Adapter for 1 culture tube 15 ml no. 15115, 1 set = 2 pcs. 

13062 

Adapter for 1 Greiner tube  15 ml, Ø 17/22 x 95 - 100 mm, 1 set = 2 pcs., suitable for 12150 

13079 

Bottom adapter for 1 tube 50 ml, e.g. no. 15051, 15052, 1 set = 2 pcs. 

13080 

Adapter for 1 culture tube 50 ml, e.g. no. 15151, 1 set = 2 pcs. 

13081 

Adapter for 1 culture tube 15 ml, e.g. no. 15115, 1 set = 2 pcs. 

13082 

Adapter for 1 tube 50 ml, max. Ø 28.8 x 105 - 115 mm, e.g. no. 13055, 15051, 15052, 1 set = 2 pcs. 

13083 

Adapter for 1 tube 30 ml, max. Ø 25.5 x 90 - 100 mm, e.g. no. 15029, 15030, 15032, 1 set = 2 pcs. 

13084 

Adapter, for 2 tubes 10 ml, Ø 16/17.5 x 75 - 90 mm, e.g. no. 15000, 15010, 15039, 1 set = 2 pcs. 

13085 

Stainless steel tube 85 ml, Ø 38 x 103 mm, sealable with cap no. 17185, suitable for 12155, 12159 

13255 

Stainless steel bottle 250 ml, Ø 61.4 x 125 mm, sealable with cap no. 17256 

 

The complete list of accessories can be downloaded from 
www.sigma-zentrifugen.de. 

 

Pos :  216  /010 Uni vers almodul e/ Seit enwechs el  @  0\ mod_1202116244312_0.doc x @  105 @   @  1 

Pos :  217  /100 Sig ma/110 BA Z entrif ugen Sig ma (Proj ekt e)/ 3-18KS-3- 18KH S/ 110  Anhang/ 110-0010- 0010 H öchst drehzahl en  von  Gefäß en  3-18KS-KH S @  29\ mod_1405321844430_68.doc x @  197841 @  3  @  1 

 

11.1.1  Maximum speed for tubes 

 Some tubes, such as centrifuge glass tubes, microtubes, culture tubes, 
fluoropolymer tubes and especially high-volume tubes can be used in our 
rotors, buckets, and adapters at higher speeds than their breaking limit. 
•  Always fill the tubes up to their useful volume (= the volume that is 

stated for the tube). 

•  When using glass tubes, the maximum value of 4,000 x g must not be 

exceeded (except special high-strength glass tubes; please refer to the 
information provided by the manufacturer). 

Pos :  218  /010 Uni vers almodul e/                                                                                Leerz eile  @  0\ mod_1202116244500_0.doc x @  114 @   @  1 

 

 

Pos :  219  /100 Sig ma/100 BA Z entrif ugen Sig ma (St andardmodul e)/110  Anhang/ 110-0010- 0020 R adi en der R ot oren ( WR+AR)  @  28\mod_1405320700923_68.doc x @  195083 @  3  @  1 

 

 

Summary of Contents for 3-18KS

Page 1: ...lation of the original operating manual sb 08 2012 Rev 1 15 of 15 09 2017 Sigma 3 18KS part no 0701102 Sigma 3 18KS from serial no 146139 Refrigerated Centrifuge Operating Manual Please retain for later use ...

Page 2: ...Refrigerated Centrifuge Sigma 3 18KS 2 89 Version 08 2012 Rev 1 15 of 15 09 2017 sb Translation of the original operating manual part no 0701102 ...

Page 3: ...perating manual part no 0701102 In case of inquiries please state the following numbers Order number Serial number Copyright by Sigma Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode am Harz Germany Tel 49 0 5522 5007 0 Fax 49 0 5522 5007 12 Web www sigma zentrifugen de E mail info sigma zentrifugen de ...

Page 4: ...Refrigerated Centrifuge Sigma 3 18KS 4 89 Version 08 2012 Rev 1 15 of 15 09 2017 sb Translation of the original operating manual part no 0701102 ...

Page 5: ... 2 2 2 2 Density 14 3 Safety 15 3 1 Marking of the unit 15 3 2 Explanation of the symbols and notes 16 3 3 Responsibility of the operator 17 3 4 Operating personnel 17 3 5 Informal safety instructions 17 3 6 Safety instructions 18 3 6 1 Electrical safety 18 3 6 2 Mechanical safety 18 3 6 3 Fire prevention 19 3 6 4 Chemical and biological safety 19 3 6 5 Safety instructions for centrifugation 20 3 ...

Page 6: ...27 6 2 2 2 Installation of angle rotors with a hermetically sealed lid 28 6 2 2 3 Installation of accessories 29 6 2 2 4 Adapters 30 6 2 2 5 Tubes 30 6 3 Control system Spincontrol S 31 6 3 1 User interface 31 6 3 2 Manual mode 32 6 3 2 1 Starting a centrifugation run 32 6 3 2 2 Interrupting a centrifugation run 32 6 3 2 3 Interrupting a deceleration process 32 6 3 2 4 Selection display and modifi...

Page 7: ... 1 4 Load bearing bolts 60 8 1 5 Glass breakage 61 8 2 Sterilisation and disinfection of the rotor chamber and accessories 61 8 2 1 Autoclaving 62 8 3 Service 63 8 4 Return of defective parts 64 9 Disposal 66 9 1 Disposal of the centrifuge 66 9 2 Disposal of the packaging 66 10 Technical data 67 10 1 Ambient conditions 68 10 2 Technical documentation 68 11 Appendix 69 11 1 Range of accessories 69 ...

Page 8: ...Refrigerated Centrifuge Sigma 3 18KS Table of contents 8 89 Version 08 2012 Rev 1 15 of 15 09 2017 sb Translation of the original operating manual part no 0701102 ...

Page 9: ...rdmodule 010 Allgemeine Infor mationen 010 0020 Besti mmungsgemäße Ver wendung 26 mod_1405318951312_68 docx 191709 2 1 1 2 Intended use Centrifuges are power driven machines that separate liquids from solid matter liquid mixtures or solid mixtures by centrifugal force They are solely intended for this purpose Any other use beyond this area of application is regarded as improper use Sigma Laborzent...

Page 10: ...r mati onen 010 0050 N or men und Vorschriften 26 mod_1405318958202_68 docx 191765 2 1 1 5 Standards and regulations EC declaration of conformity see chapter 11 6 EC declaration of conformity Pos 11 010 U ni versal module Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 12 100 Sigma 110 BA Zentrifugen Sigma Pr ojekte 3 18KS 3 18KH S 010 Allgemeine Infor mationen 010 0060 Li eferumfang 3 18KS_3 18KH ...

Page 11: ...U ni versal module Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 18 100 Sigma 110 BA Zentrifugen Sigma Pr ojekte 3 18KS 3 18KH S 020 Aufbau und Wir kungsweise 020 0010 0010 Funkti ons und Bedienelemente 3 18KS 29 mod_1405321768977_68 docx 197589 3 1 2 1 1 Functional and operating elements 1 Lid 2 User interface see chapter 6 3 1 User interface 3 Mains switch Fig 1 Total view of the centrifuge 4 N...

Page 12: ... registered office 2 Type 3 Serial number 4 Refrigerant data 5 Max density 6 Nominal voltage 7 Input fuse 8 Symbol for special disposal see chapter 9 Disposal 9 CE mark in compliance with the directive 2006 42 EC 10 Part number 11 Year of manufacture 12 Max speed 13 Max kinetic energy 14 Power consumption Fig 3 Example of a name plate Pos 21 010 U ni versal module Seitenwechsel 0 mod_1202116244312...

Page 13: ... function of the angular velocity The bigger the radius in the rotor chamber is and the higher the speed is the higher the centripetal acceleration is However the forces acting on the rotor also increase Pos 24 010 U ni versal module Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 25 100 Sigma 100 BA Zentrifugen Sigma Standar dmodule 020 Aufbau und Wir kungsweise 020 0020 0020 Anwendungsbereich 26 ...

Page 14: ...rce will be recalculated automatically by the control unit If the RCF is changed the speed will be adapted while the specified radius is maintained The speed gravitational field diagram provides an overview of the relationship between speed radius and RCF see chapter 11 2 Speed gravitational field diagram Pos 28 010 U ni versal module Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 29 100 Sigma 100...

Page 15: ... Sigma Dangerous voltage I On Power Hot surface 0 Off Power Caution Risk of bruising Name plate see chapter 2 1 2 Name plate Protective earth ground CE mark in compliance with the directive 2006 42 EC Earth ground Do not dispose as part of domestic waste Unplug the mains plug GS mark tested safety only for Germany Arrow indicating the direction of rotation NRTL mark only for the USA and Canada 4 C...

Page 16: ...fe and health of persons Non observance of these symbols causes serious health problems up to life endangering injuries DANGER This symbol stands for a direct hazard to the life and health of persons due to electrical voltage Non observance of these symbols causes serious health problems up to life endangering injuries WARNING This symbol stands for a potential hazard to the life and health of per...

Page 17: ... Pos 39 010 U ni versal module Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 40 100 Sigma 100 BA Zentrifugen Sigma Standar dmodule 030 Sicher heit 030 0040 Bedienpersonal 26 mod_1405319017634_68 docx 191989 2 1 3 4 Operating personnel Persons operating the unit must be familiar with the fundamental regulations concerning workplace safety and accident prevention have read and understood this opera...

Page 18: ...cher heit 030 0060 0020 M echanische Sicher heit außer 1 16 1 16K 26 mod_1405319024314_68 docx 192059 3 1 3 6 2 Mechanical safety In order to ensure the safe operation of the centrifuge observe the following WARNING Do not open the lid when the rotor is in motion Do not reach into the rotor chamber when the rotor is in motion Do not use the centrifuge if it was installed incorrectly Do not use the...

Page 19: ... Sigma Standar dmodule 030 Sicher heit 030 0060 0040 C hemische und bi ologische Sicher hei t 26 mod_1405319028141_68 docx 192101 3 1 3 6 4 Chemical and biological safety If pathogenic toxic or radioactive samples are intended to be used in the centrifuge it is in the responsibility of the user to ensure that all necessary safety regulations guidelines precautions and practices are adhered to acco...

Page 20: ...rsting vessels can cause dangerous imbalances at high speeds Ensure that rotor and buckets are correctly fitted see chapter 6 2 2 1 Installation of the rotor Observe the instructions on the installation of accessories see chapter 6 2 2 3 Installation of accessories WARNING The rotor must be loaded axial symmetrically at equal weights If liquids with a density 1 2 g cm3 are used reduce the speed se...

Page 21: ... month for safety reasons Pay special attention to changes such as corrosion cracks material abrasion etc After 10 years they must be inspected by the manufacturer After 50 000 cycles the rotor must be scrapped for reasons of safety If other data concerning the service life are engraved on the rotor or bucket these data shall apply accordingly For example a bucket with the engraving max cycles 10 ...

Page 22: ...t 12 mod_1337063062114_0 docx 65687 1 Pos 67 100 Sigma 100 BA Zentrifugen Sigma Standar dmodule 030 Sicher heit 030 0070 0040 Schutzleiterpr üfung Spincontrol L S Easy Uni versal 27 mod_1405319043129_68 docx 192241 3 1 3 7 4 Earth conductor check For the earth conductor check there is an equipotential bonding screw on the rear panel of the centrifuge see chapter 2 1 1 Functional and operating elem...

Page 23: ...00 BA Zentrifugen Sigma Standar dmodule 030 Sicher heit 030 0100 R estrisi ken 27 mod_1405319052885_68 docx 192339 2 1 3 9 Remaining hazards The unit was built state of the art and according to the accepted safety rules However danger to life and limb of the operator or of third parties or impairments of the unit or other material assets cannot be completely excluded when the unit is being used Us...

Page 24: ...9161442_68 docx 192367 2 1 4 2 Storage conditions The centrifuge can be stored in its original packaging for up to a year Store the centrifuge only in dry rooms The permissible storage temperature is between 20 C and 60 C If you would like to store it for more than one year or if you intend to ship it overseas please contact the manufacturer Pos 84 010 U ni versal module Leerzeile 0 mod_1202116244...

Page 25: ...KS 3 18KH S 040 Lagerung und Tr ansport 040 0050 Transportsicherung 3 18KS_KHS Überschrift 29 mod_1405321789957_68 docx 197701 2 1 4 5 Transport safety device Pos 90 100 Sigma 110 BA Zentrifugen Sigma Pr ojekte 3 18KS 3 18KH S 040 Lagerung und Tr ansport 040 0051 Transportsicherung 3 18KS_KHS Text 36 mod_1434605924079_68 docx 263917 1 The transport safety device consists of a foamed plastic piece ...

Page 26: ...d_1202116244500_0 docx 114 1 Pos 96 100 Sigma 100 BA Zentrifugen Sigma Standar dmodule 050 Aufstellung und Anschl uss 050 0020 Energieversorgung 27 mod_1405319175630_68 docx 192409 2 1 5 2 Power supply Pos 97 100 Sigma 100 BA Zentrifugen Sigma Standar dmodule 050 Aufstellung und Anschl uss 050 0020 0011 Anschl ussart Kaltger ätestecker Schutzschalter 32 mod_1417600691402_68 docx 222498 3 1 5 2 1 T...

Page 27: ...2465 3 1 6 2 1 Opening and closing the lid The lid can be opened if the centrifuge is at a standstill and if the lid key is illuminated Press the lid key in order to open the lid The centrifuge cannot be started if the lid is opened To close press with both hands slightly on the lid until the electrical lock is locked WARNING Do not place your fingers between the lid and the housing when closing t...

Page 28: ... 0020 0020 0020 3 18KS_KH S Ei nsetzen von Wi nkelrotoren mit her metisch verschli eßbarem Deckel 29 mod_1405321818963_68 docx 197771 4 1 6 2 2 2 Installation of angle rotors with a hermetically sealed lid Screw the rotor cover onto the rotor and tighten it Lower the rotor with the cover onto the motor shaft Insert the rotor tie down screw into the motor shaft Tighten the rotor tie down screw with...

Page 29: ...ymmetrical inserts buckets of the rotors with the same accessories and fill to avoid imbalance Centrifugation with different tube sizes Working with different tube sizes is possible In this case however it is very important that axial symmetrical inserts are identical correct incorrect Fig 6 Permissible and impermissible loading of a swing out rotor with different tube sizes example illustrations ...

Page 30: ... docx 114 1 Pos 118 100 Sig ma 100 BA Zentrifugen Sig ma Standardmodul e 060 Betrieb_Spi ncontr ol L S 060 0020 0020 0050 Gefäße außer 4 5L 27 mod_1405319211622_68 docx 192577 4 1 6 2 2 5 Tubes Load the tubes outside of the centrifuge Liquids in the buckets or multiple carriers cause corrosion Fill the tubes carefully and arrange them according to their weight Imbalances result in the excessive we...

Page 31: ...20193718_68 docx 193123 3 1 6 3 1 User interface The centrifuge is operated via three buttons with integrated light emitting diodes and one function knob The display is divided into several different fields The various functions of the system can be called up by pressing and turning the function knob 1 Start button 2 Display 3 Function knob 4 Stop button 5 Lid button Fig 8 User interface of the Sp...

Page 32: ...ust be opened before a new centrifugation run can be started A quick stop can also be triggered during a normal deceleration e g in order to speed up the deceleration When a quick stop is triggered Quick stop will be displayed in the speed field NOTE A quick stop can be performed even if the centrifuge is blocked against unauthorised use Pos 127 010 Uni versalmodul e Leerzeile 0 mod_1202116244500_...

Page 33: ...lues are stated in revolutions per minute min 1 rpm and depend on the RCF values see chapter 2 2 2 1 Speed radius and relative centrifugal force The maximum speed values depend on the rotor that is used Relative centrifugal force RCF The relative centrifugal force is the acceleration that the sample is subjected to during the centrifugation run The set value of this parameter is displayed in the u...

Page 34: ...eached Select the field Runtime and confirm the selection The display flashes when it is activated Turn the function knob from the time 0 00 10 anti clockwise or from the time 99 59 59 clockwise Infinite will be displayed After the start of the centrifuge the elapsed time will be displayed Deactivate the continuous run by pressing the stop button or by entering a specific runtime Short run A short...

Page 35: ...he data Fig 11 Rotor selection list here with potential rotor bucket combinations and additional information Automatic rotor identification The centrifuge automatically identifies the rotor that is currently being used If the system identifies a different rotor than the one that is set and if there are no different buckets for this rotor the rotor input will be adapted automatically The system wil...

Page 36: ...menu shows the program that is currently loaded When the field is selected the program list is displayed for information on how to work with the programs please see chapter 6 3 3 Program mode The program RAPID_TEMP see below cannot be deleted Fig 12 Program list Program RAPID_TEMP Precooling or preheating at a standstill may distort the measurement results and cause the increased wear of the mecha...

Page 37: ...his case the RAPID_TEMP program stops with a sound signal and the standstill cooling system will be activated The stop button is pressed The RAPID_TEMP program will be stopped prematurely No message will be issued when the set temperature is reached A parameter is changed except for the temperature or another input is made In this case the RAPID_TEMP program will be aborted No message will be issu...

Page 38: ...the actual sample temperature Progress indicator The progress indicator provides a quick overview of the remaining runtime of the running centrifugation run For this purpose a green progress bar and percentage value are displayed in the program field Fig 14 Progress indicator during a centrifugation run After the completion of the centrifugation run the progress indicator remains at 100 until the ...

Page 39: ...mperature exception the RAPID_TEMP program will not be listed The order of the processes corresponds to their storage locations on the program list Empty storage locations will not be displayed If more than 11 programs have been stored the user can scroll through the list Fig 15 Process library menu Loading a process In the process library select the desired process by turning the function knob Pr...

Page 40: ...cooled samples in the uncooled centrifuge from heating up to an inadmissible temperature level If the standstill cooling function is activated the centrifuge starts to precool after it is switched on In the runtime field the symbol is displayed The lid must be closed CAUTION Unmoved air in the rotor chamber leads to an incorrect measuring and control behaviour and causes the compressor to freeze o...

Page 41: ...toring function enables the continuous monitoring of the speed and runtime parameters during the centrifugation Activate the centrifugation monitoring function by clicking NOTE If the function is activated during a centrifugation run the monitoring process will not be started until the start of the next centrifugation run The centrifugation monitoring function compares the speed values of the curr...

Page 42: ...current temperature is between the preset temperature in the Standard menu and the Delta T limit value If the temperature leaves this range during the centrifugation run an error message will be displayed and the centrifuge will be decelerated to a complete standstill NOTE The delta T temperature monitoring system remains inactive as long as the RAPID_TEMP program is active see chapter 6 3 2 5 Sta...

Page 43: ...nction will also be selected Select the button Activate code Enter a four digit code and confirm the entry The lock is now active The symbol will be displayed in the status line and the lock level will be indicated If changes are made after a function has been blocked the system will ask for the code prior to executing the change Fig 18 Parameters menu with an active lock level 01 Unblocking a fun...

Page 44: ...bles the optimum adaptation of the centrifuge to its specific area of application Fig 19 Menu Setup Function Open lid after run The automatic lid opening function ensures that the lid opens when the rotor has stopped CAUTION When the lid is open the cooling is not active The sample may warm up Program rotation See chapter 6 3 3 5 Automatic program rotation Runtime as of set speed If this function ...

Page 45: ...n be displayed in C Celsius or F Fahrenheit Language The control system can be used in various language versions If a language is selected by mistake it can be changed on any screen as follows Press and hold the stop button Turn the function knob one notch to the left and then one notch to the right Release the stop button The Language window will be displayed Select the desired language Speed RCF...

Page 46: ...when the cursor flashes in the text field Turn the function knob in order to select a character and press it to confirm the selection Then press the knob again in order to enter the next character Pressing the arrow button will delete the last character The maximum number of characters is 19 When the name is complete select the option Accept and confirm it Buzzer signal With this function a warnin...

Page 47: ...rve editor will be displayed Select a curve number between 20 and 29 in the input field Curve If the curve number is already used the stored curve will be displayed The input field Int is used to specify the interval number of the process Up to ten intervals can be entered for a curve Enter the interval time of the current interval into the input field Time While doing so certain restrictions must...

Page 48: ... necessary slope 0 03 min 1 rpm which means that it is beyond the defined range Example 2 Start speed 0 min 1 rpm final speed 15000 min 1 rpm runtime 10 sec not possible since the necessary slope is 1500 min 1 rpm which means that it is beyond the defined range Pos 141 010 Uni versalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 142 100 Sig ma 100 BA Zentrifugen Sig ma Standardmodul e 060 ...

Page 49: ...114 1 Pos 147 100 Sig ma 100 BA Zentrifugen Sig ma Standardmodul e 060 Betrieb_Spi ncontr ol L S Spincontrol S Zent7 060 0030 0030 Programmbetri eb 27 mod_1405320235093_68 docx 193459 3 1 6 3 3 Program mode A program contains all of the data that are required for a centrifuge run Certain sedimentation results can be repeated under identical conditions Programs can be loaded executed edited and del...

Page 50: ... the selection Then the next character can be selected Pressing the arrow button will delete the last character When the program name is complete select OK and confirm it The program will be saved and the Standard menu will be displayed Fig 23 Assignment of a program name prior to saving the program Pos 150 010 Uni versalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 151 100 Sig ma 100 BA ...

Page 51: ...onfirm the selection by pressing the function knob Press the start button Or Open the process library select the desired program and press the start button The program will be executed and the Standard menu will be displayed Pos 154 010 Uni versalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 155 100 Sig ma 100 BA Zentrifugen Sig ma Standardmodul e 060 Betrieb_Spi ncontr ol L S Spincontrol...

Page 52: ...tup menu Fig 26 Program rotation function When a program is loaded while the program rotation function is active this program will be used as the start program for the rotation After the completion of the program the next program on the program list will be loaded automatically The rotation continues up to the next empty storage location and then restarts from the beginning see the following illus...

Page 53: ... centrifuge type partly standard External signal active DC 24V 0 5 A max part no 17701 Floating switch AC 250V max 6 A part no 17702 Pos 160 010 Uni versalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 161 100 Sig ma 100 BA Zentrifugen Sig ma Standardmodul e 060 Betrieb_Spi ncontr ol L S 060 0040 Ausschalten 27 mod_1405319212716_68 docx 192591 2 1 6 4 Switching the centrifuge off Open the ...

Page 54: ...ectly Fuses have tripped Reactivate temperature fuse see chapter 5 2 1 Type of connection Mains power switch off Switch mains power switch on Centrifuge cannot be started start key LED is not illuminated Several Power off on If the error occurs again contact service Centrifuge cannot be started lid key LED flashes The lid lock is not closed correctly Open and close the lid If the error occurs agai...

Page 55: ... the socket Remove the plug see figure item 1 from the opening on the right side panel e g with a screwdriver Fig 28 Position of the opening for the emergency lid release First pull the red string to release the mechanism and then unlock the lid lock by pulling the white string Then reinsert the plug and quit the error message by switching the centrifuge off on Approximately 10 seconds after quitt...

Page 56: ...frigerated centrifuges Allow to slow down Power off Allow to cool down Provide better ventilation only air cooled centrifuges Provide sufficient water throughput only water cooled centrifuges 46 49 Imbalance error only for centrifuges with imbalance monitoring system Allow to slow down Power off Eliminate the imbalance 50 59 Lid error Press lid key Close lid Remove foreign matter from the opening ...

Page 57: ...ries malfunctions or spare part enquiries From Germany Contact Sigma Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode Germany Tel 49 0 55 22 50 07 44 44 E mail support lab sigma zentrifugen de Outside Germany Contact our agency in your country All agencies are listed at www sigma zentrifugen de Sales Partners NOTE If you would like to utilise our service please state the type of your ce...

Page 58: ... g by heat generators Pos 175 010 Uni versalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 176 100 Sig ma 100 BA Zentrifugen Sig ma Standardmodul e 080 War tung und Instandhaltung 080 0010 Wartungsar beiten 28 mod_1405320613978_68 docx 194803 2 1 8 1 Maintenance Pos 177 100 Sig ma 100 BA Zentrifugen Sig ma Standardmodul e 080 War tung und Instandhaltung 080 0010 0010 Zentrifuge 28 mod_1405...

Page 59: ...20 Zubehör außer 8K 28 mod_1405320618247_68 docx 194845 3 1 8 1 2 Accessories CAUTION For the care of the accessories special safety measures must be considered as these are measures that will ensure operational safety at the same time Immediately rinse off the rotor buckets or accessories under running water if they have come into contact with any liquids that may cause corrosion Use a brush for ...

Page 60: ...affect or destroy the metals Barely detectable cracks on the surface can expand and weaken the material without any visible signs Check the material regularly at least once a month for cracks visible damage of the surface pressure marks signs of corrosion other changes Check the bores of the rotors and multiple carriers Replace any damaged components immediately for your own safety After every cle...

Page 61: ... upper third of the rotor chamber with e g Vaseline Then let the rotor rotate for a few minutes at a moderate speed approx 2000 rpm The glass and metal particles will now collect at the greased part Remove the grease with the glass and metal particles with a cloth If necessary repeat this procedure Pos 190 010 Uni versalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 191 100 Sig ma 100 BA Z...

Page 62: ...es Max temp C Min time min Max time min Max cycles Aluminium buckets 134 138 3 5 Aluminium rotors 134 138 3 5 Glass tubes 134 138 3 40 Polyallomer polycarbonate rectangular carriers 115 118 30 40 Polyallomer polycarbonate round carriers 115 118 30 40 Polyamide buckets 115 118 30 40 10 Polycarbonate polyallomer lids for angle rotors 115 118 30 40 20 Polycarbonate tubes 115 118 30 40 20 Polyphenylsu...

Page 63: ...f the centrifuge Nevertheless wear cannot be excluded and it may not be visible from the outside Especially the rubber parts that are among other things part of the motor suspension are subject to ageing This is why we recommend having the centrifuge checked by the manufacturer during an inspection once per year in the operating state and once every three years in the dismantled state Rubber parts...

Page 64: ...ged to certify the harmlessness of all incoming goods For this purpose we require a declaration of decontamination The form must be filled in completely and signed by authorised and specialised personnel only Affix the original form in a clearly visible manner to the outside of the packaging NOTE We will return the part unit if no declaration of decontamination is provided 2 Form for the return of...

Page 65: ... Please use the original packaging for the unit if at all possible If the product is dispatched to us in unsuitable packaging you will be charged the cost for returning it to you in new packaging The forms can be downloaded online from www sigma zentrifugen de Service Overhaul and repair Pos 198 010 Uni versalmodul e Abschnittswechsel 0 mod_1202124514062_0 docx 418 1 Pos 199 010 Uni versalmodul e ...

Page 66: ...household waste You can return these centrifuges free of cost to Sigma Laborzentrifugen GmbH Ensure that the unit is decontaminated Fill in a declaration of decontamination see chapter 8 4 Return of defective parts Comply with any other applicable local rules and regulations Pos 202 010 Uni versalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 203 100 Sig ma 100 BA Zentrifugen Sig ma Standa...

Page 67: ... V 50 Hz 16 0 at 220 240 V 50 Hz temperature fuses Performance data Max speed rpm Max capacity ml Max gravitational field x g Max kin energy Nm 18 000 1 600 30 070 22 997 Other parameters Time range Temperature range Storage locations 10 sec 99 h 59 min short run continuous run 20 C to 40 C 60 Physical data Height mm Height with open lid mm Width mm Depth mm Weight kg 355 785 630 600 78 Noise leve...

Page 68: ...ive humidity of air at 35 C Maximum altitude 2 000 m above sea level Pos 210 010 Uni versalmodul e Seitenwechsel 0 mod_1202116244312_0 docx 105 1 Pos 211 100 Sig ma 100 BA Zentrifugen Sig ma Standardmodul e 100 Technische D aten 100 0020 Technische Dokumentation 28 mod_1405320681369_68 docx 195055 2 1 10 2 Technical documentation For environmental reasons the comprehensive technical documentation ...

Page 69: ...ngular carriers no 18015 and 48 PPCO tubes 15 ml no 15023 Radius max 17 1 cm radius min 7 6 cm 4 700 4 223 11182 Swing out rotor 12 x 50 ml culture tubes complete no 11180 incl 2 sets buckets no 13190 2 sets adapters no 17346 and 12 tubes no 15151 Radius max 17 2 cm radius min 7 5 cm 4 700 4 248 11222 Swing out rotor for microtiter plates incl 1 set carriers no 13222 max height of plates 56 mm max...

Page 70: ...ngle rotor 12 x 5 ml Eppendorf reaction caps incl hermetic aluminium lid no 17182 software version50 or higher Radius max 8 4 cm radius min 4 3 cm 17 500 28 761 12349 Angle rotor 48 x 1 5 2 0 ml for reaction vials e g no 15008 15040 incl hermetic lid 17349 Radius max 10 0 cm radius min 6 5 cm 15 000 25 155 19776 Angle rotor for 6 x 50 ml culture tubes e g no 15151 incl hermetic aluminium lid no 17...

Page 71: ...for 1 tube 30 ml max Ø 25 5 x 90 100 mm e g no 15029 15030 15032 1 set 2 pcs 13084 Adapter for 2 tubes 10 ml Ø 16 17 5 x 75 90 mm e g no 15000 15010 15039 1 set 2 pcs 13085 Stainless steel tube 85 ml Ø 38 x 103 mm sealable with cap no 17185 suitable for 12155 12159 13255 Stainless steel bottle 250 ml Ø 61 4 x 125 mm sealable with cap no 17256 The complete list of accessories can be downloaded from...

Page 72: ...on in the accessories table concerning the radius refers to the values of the respective rotor as shown below The radius calculation is described in chapter 2 2 2 1 Speed radius and relative centrifugal force Fig 29 Minimum and maximum radius of an angle rotor Fig 30 Minimum and maximum radius of a swing out rotor Pos 220 010 Uni versalmodul e Seitenwechsel 0 mod_1202116244312_0 docx 105 1 ...

Page 73: ...g manual part no 0701102 Pos 221 100 Sig ma 100 BA Zentrifugen Sig ma Standardmodul e 110 Anhang 110 0020 Dr ehzahl Schwer efeld Diagr amm 28 mod_1405320705642_68 docx 195153 2 1 11 2 Speed gravitational field diagram Fig 31 Speed gravitational field diagram Pos 222 010 Uni versalmodul e Seitenwechsel 0 mod_1202116244312_0 docx 105 1 ...

Page 74: ...he fixed acceleration curves defines the time that is required to accelerate the rotor by 1 000 rpm Curve 9 is a special case compared to the other curves The centrifuge accelerates with maximum power The runtime until the set speed is reached depends solely on the moment of inertia of the rotor Linear curve no Slop 0 4 rpm sec 1 6 rpm sec 2 8 rpm sec 3 17 rpm sec 4 25 rpm sec 5 33 rpm sec 6 50 rp...

Page 75: ...ersion 08 2012 Rev 1 15 of 15 09 2017 sb 75 89 Translation of the original operating manual part no 0701102 Fig 34 Diagram of linear curves Fig 35 Diagram of quadratic curves Pos 224 010 Uni versalmodul e Seitenwechsel 0 mod_1202116244312_0 docx 105 1 ...

Page 76: ...K 12101 5 years 20x 1 15 1 15K 1 15P 1 15PK 12124 5 years 20x 1 15 1 15K 1 15P 1 15PK 12126 5 years 20x 1 15 1 15K 1 15P 1 15PK 12134 5 years 20x 1 16 1 16K 12135 5 years 20x 1 16 1 16K 12137 5 years 20x 1 16 1 16K 12500 7 years 6 15 6K15 6 16 6 16K 12600 7 years 6 16S 6 16KS 13218 20 000 4 16 4 16S 4 16K 4 16KS 6 16 6 16S 6 16K 6 16KS 13296 5 years 10x 2 6 2 6E 2 7 13299 5 years 10x 2 6 2 6E 2 7 ...

Page 77: ...1 Acetone C3H6O 100 1 1 4 1 1 4 4 1 4 1 Acrylonitrile C3H3N 100 1 1 4 3 3 4 4 4 1 4 1 Allyl alcohol C3H6O 96 1 3 3 2 2 2 2 4 1 1 1 Aluminium chloride AlCl3 saturated 1 3 2 4 1 1 1 1 4 Aluminium sulfate Al2 SO4 3 10 1 1 1 3 1 1 1 1 1 1 1 Ammonium chloride NH4 Cl aqueous 1 1 1 2 1 1 1 1 1 1 3 Ammonium hydroxide NH3 H2O 30 1 3 4 1 1 2 1 1 1 Aniline C6H7N 100 1 3 4 1 2 4 4 4 1 4 1 Anisole C7H8O 100 3 ...

Page 78: ...ane C2H8N2 100 1 1 3 1 1 3 4 1 1 1 Diesel fuel 100 1 1 3 1 1 1 3 1 1 1 Dimethyl formamide DMF C3D7NO 100 1 1 4 1 1 4 3 1 3 1 Dimethyl sulfoxide DMSO C2H6SO 100 1 2 4 1 1 4 4 1 1 Dimethylaniline C8H11N 100 3 4 2 4 1 1 Dioxane C4H8O2 100 2 1 4 1 3 2 3 4 1 3 1 Dipropylene glycol mono methyl ether C4H10O 100 3 1 4 1 4 4 4 4 1 1 Ethyl acetate C4H8O2 100 1 1 4 1 1 4 4 4 1 4 1 Ethylene chloride C2H4Cl2 1...

Page 79: ...1 3 1 2 1 Methyl benzene C7H8 100 3 1 4 1 3 4 4 4 1 4 1 Methyl ethyl ketone MEK C4H8O 100 1 1 4 1 1 4 4 4 1 4 1 Methylene chloride CH2Cl2 100 4 3 4 3 3 4 4 4 1 1 Mineral oil 100 1 1 1 1 1 1 1 1 1 1 Nitric acid HNO3 10 1 4 1 4 1 1 1 1 4 3 Nitric acid HNO3 100 4 4 4 4 4 4 1 4 1 Nitrobenzene C6H5NO2 100 3 4 4 3 2 4 4 4 1 4 1 Oleic acid C18H34O2 100 1 1 1 2 1 1 1 3 1 Oxalic acid C2H2O4 x 2H2O 100 1 3 ...

Page 80: ... 1 1 1 1 1 2 4 Sodium hydroxide NaOH 50 1 1 4 1 1 1 1 1 2 4 Sodium sulfate Na2SO4 10 1 1 1 1 1 1 1 1 1 1 1 Spirits C2H6O 96 1 1 1 1 1 1 1 3 1 1 Styrene C8H8 100 4 1 4 1 3 4 4 1 4 1 Sulphuric acid H2SO4 6 1 4 1 4 1 1 1 1 2 3 Sulphuric acid H2SO4 fuming 4 4 4 4 4 4 4 4 1 4 3 Tallow 100 1 1 1 1 1 1 1 1 1 1 Tetrahydrofuran THF C4H8O 100 3 1 4 1 3 4 4 4 1 3 1 Tetrahydronaphthalene C10H12 100 3 1 4 1 4 ...

Page 81: ...5 09 2017 sb 81 89 Translation of the original operating manual part no 0701102 Pos 229 100 Sig ma 110 BA Zentrifugen Sig ma Proj ekte 3 18KS 3 18KH S 110 Anhang 110 0060 Konfor mitätser kl ärung 3 18KS_KHS 29 mod_1405321845824_68 docx 197855 2 1 11 6 EC declaration of conformity ...

Page 82: ...Refrigerated Centrifuge Sigma 3 18KS 11 Appendix 82 89 Version 08 2012 Rev 1 15 of 15 09 2017 sb Translation of the original operating manual part no 0701102 ...

Page 83: ...Refrigerated Centrifuge Sigma 3 18KS 11 Appendix Version 08 2012 Rev 1 15 of 15 09 2017 sb 83 89 Translation of the original operating manual part no 0701102 11 7 Declaration of conformity China RoHS 2 ...

Page 84: ...Refrigerated Centrifuge Sigma 3 18KS 11 Appendix 84 89 Version 08 2012 Rev 1 15 of 15 09 2017 sb Translation of the original operating manual part no 0701102 Ende der Liste für Textmar ke Inhalt ...

Page 85: ...trifugation monitoring 41 Centrifugation of infectious toxic radioactive or pathogenic substances 19 Centrifugation principle 13 Centrifugation with different tube sizes 29 Centrifugation with low capacity 29 Centrifuge cannot be started 54 Centrifuge decelerates during operation 54 Centrifuge is inclined 54 Centrifuge was moved during run 54 Centrifuge cleaning and care 58 Centrifuges definition ...

Page 86: ...cy lid release 55 Equipotential bonding screw 22 Error correction 54 Error message 54 Executing a program 51 Explanation of the symbols and notes 16 Explosive substances 19 External signal 46 F Filling quantity refrigerant 67 Fire preventions 19 Form for the return of defective parts 64 Function 44 Functional and operating elements 11 Fuses have tripped 54 G General conditions 9 Glass breakage 61 ...

Page 87: ...r shaft 27 28 58 Multiple carrier 30 N Name plate 11 12 No indication on the display 54 No power in the mains supply 54 Noise level 67 Nominal voltage 12 Non greased bolts 60 Notes on safety and hazards 9 Notes on transport 24 O Online download of forms 65 Open lid after run 44 Opening the lid 27 Operating personnel 17 Operating voltage 26 Operational safety 59 Option for the input and output of d...

Page 88: ...s 21 Safety chemical and biological 19 Safety electrical 18 Safety mechanical 18 Safety conscious work 17 Saving a program 50 Scope of supply 10 Selection display and modification of data 32 Serial number 12 57 63 Service 63 Service contact 57 Service life 58 Service life of rotors and accessories 21 76 Service life of the accessories 62 Service work 63 Set up and connection 26 Setup menu 44 Short...

Page 89: ...Temperature value not reached 54 Thermal stress 58 Time fine 45 Time range 67 Toxic substances 19 58 Transport safety device 25 Tubes 30 Type 12 Type of connection 26 Type of the centrifuge 57 63 67 U Unblocking a function 43 Ungreased load bearing bolts 54 Useful volume volume that is stated for the tube 30 71 User interface 31 UV radiation 26 58 V Vents 26 Vessels 30 W Warning signal 46 Warranty...

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