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Sigma 3-16PK, Use And Maintenance Manual

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Sigma 3-16PK Use And Maintenance Manual Download Page 21

CONTROLLED COPY 

 Centre for Biological Engineering 

 

Standard Operating Procedure  

 

 

 

 

 

 

 

SOP122 

 

Title:  Use and Maintenance of Sigma Refrigerated Centrifuge 3-16PK

 

 

Location: 

CBE Laboratory Unit (H21/22) 

 

Version 003   

Effective Date:  26

th

 June 2018 

Review 26

th

 June 2020 

 
Written by: Victoria Workman 
 

Reviewed by: P.Hourd 

Approved by: R/I.Temple 

 
 

Page 21 of 22 

 

 

Remember that it is important to follow the manufacturer's instructions in all aspects of centrifuge 
use. Only trained persons should use centrifuges. If in doubt about the operation of a centrifuge – 
ask somebody else. 
 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

Summary of Contents for 3-16PK

Page 1: ...UST be used for applications involving the centrifugation of infectious toxic or pathogenic material SPECIAL NOTES HEALTH SAFETY 2 1 Hazards associated with use of centrifuge Mechanical failure of rot...

Page 2: ...he start If a rotor other than the preselected one is installed the system will display the installed rotor and the pre selection speed will be corrected if necessary x Use only the correct designated...

Page 3: ...ey intend to carry out iv Shall record alarms adverse events non conformances or malfunction on the Preventative Maintenance of Centrifuges Record Section 6 and notify the Laboratory Manager Responsib...

Page 4: ...efore each use NOTE Lifetime of the Rotor After 10 years the rotors and accessories must be inspected by the manufacturers The rotor must be scrapped if it has done 50 000 cycles for reasons of safety...

Page 5: ...up to the maximum recommended by the manufacturer according to the head and accessories to be used or to the recommended reduced speed if the sample has a high density viii Follow the manufacturer s i...

Page 6: ...Always consider all components e g inserts and trunnion rings when balancing There should be no more than 100g difference between buckets next to each other iii Avoid contaminating the outside of a s...

Page 7: ...2018 Review 26th June 2020 Written by Victoria Workman Reviewed by P Hourd Approved by R I Temple Page 7 of 22 iii Insert the holders into the rotor ensuring that the holders fit correctly into the t...

Page 8: ...of the option Rotor on the display ii Select the option by pressing or turning the right rotary knob Set will now flash iii Turn the right rotary knob until the desired rotor number is displayed iv P...

Page 9: ...isplayed iv Press the right rotary knob to confirm the selected value If this is not done the value will be automatically reset to the last setting v When the maximum permissible speed of the rotor is...

Page 10: ...field in front of the parameter Temp ii Select the option by pressing or turning the right rotary knob Set will now flash iii Turn the right rotary knob until the desired value is displayed iv Press t...

Page 11: ...l for continuous run v The program will only be loaded if the actual temperature is above the set temperature vi The program runs until the set value is reached Then a sound signal is issued and the p...

Page 12: ...ted with seals in place vi If the temperature of the chamber is below room temperature pre cool the rotor to the lower temperature before securing the rotor this will minimize the chance of it seizing...

Page 13: ...otate the rotor by hand without the rotor fastening nut If the motor shaft does not turn around on a perpendicular axis the motor and motor shaft must be replaced Refit the rotor correctly after visua...

Page 14: ...l grain of rice to the load bearing bolts of the rotor and the buckets vi After completion of the routine clean record the event the Preventative Maintenance of centrifuges Record Section 6 Consult th...

Page 15: ...lly removed Rubber inserts must be carefully cleaned or replaced If a problem has occurred the following must be considered Glass particles in the rubber cushion will cause glass breakage again Glass...

Page 16: ...he CBE website NOTE A Declaration of decontamination available in the Operators Manual may also be required 5 7 Centrifuge or Rotor Decommissioning Checklist i If the centrifuge or rotor needs to be d...

Page 17: ...British Standard can expel debris or even move bodily for a distance of several metres in the event of mechanical failure While considerable effort goes into the design and construction of centrifuge...

Page 18: ...manufacturer s instructions for starting and where the control is not automatic for increasing the speed of rotation B 1 4 6 Allow the rotation assembly to come to rest before attempting to open the...

Page 19: ...on of the rotor within the centrifuge The centrifugal forces cause rotors to stretch At low and normal speeds the change of size is elastic and the rotor returns to its original size when stationary A...

Page 20: ...ach bucket Centrifuging Hazardous Materials Hazardous materials must be centrifuged in the proper type of sealed container The basic principle of safe containment is an air tight leak proof seal Most...

Page 21: ...Unit H21 22 Version 003 Effective Date 26th June 2018 Review 26th June 2020 Written by Victoria Workman Reviewed by P Hourd Approved by R I Temple Page 21 of 22 Remember that it is important to follow...

Page 22: ...rms from the SOP These are now on the CBE website iii Amendment to forms The QS Form 003 Preventative Maintenance of centrifuges now referenced in place of other inspection cleaning forms iv 5 5 3 Add...

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