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Operating Manual SIGMA 3-16K, page 63 of 69

03/06

7.2.4

Emergency Lid Release

At the upper right panel there is a stopper which can be removed eg. with a
screw driver. The lid can be released by pulling the visible string.

Attention!

The lid may only be unlocked and opened when the rotor is at standstill.

7.2.5

Problems with the Centrifuge?

Please contact your supplier for support or in the event of malfunctions and for
supply of spare parts.

7.3

Error Mode

In the error mode the Start-, Stop- and Lid-keys are flashing. “ERROR“ is
displayed in the speed area and the error code in the rcf area.

7.3.1

Error Codes

Error no.

Kind of error

Actions

Tacho signal disturbed

•

Power off/on

Lid does not open after
pressing the Lid-key

•

Power off/on

•

Emergency lid release

2, 3, 5 – 11, 17 - 30 Internal fault

•

Power off/on

Imbalance determined

•

Remove imbalance

Temperature sensor

•

Contact service

Excess temperature (>50°C)

•

Let centrifuge cool down

Should it not be possible to repair the failure, please contact Service!

7.4

Speed-RCF-Diagram

An additional help is the enclosed Speed-RCF-Diagram.

Summary of Contents for 3-16K

Page 1: ... Refrigerated Centrifuge 3 16K Operating Manual ...

Page 2: ......

Page 3: ...are sure that your demands will always be fulfilled Please read this manual carefully before installation of the centrifuge to ensure a proper and safe operation We are wishing you a successful use of the centrifuge SIGMA Laborzentrifugen GmbH P O Box 1713 37507 Osterode Germany Phone 05522 5007 0 Fax 05522 500712 Internet www sigma zentrifugen de eMail info sigma zentrifugen de SIGMA Service Tel ...

Page 4: ......

Page 5: ... 336 CEE and 98 37 CEE La machine désignée ci dessous est produit et examiné conforme aux directives 73 23 CEE 89 336 CEE et 98 37 CEE Bezeichnung der Maschine Laborzentrifuge Machine Laboratory Centrifuge Désignation de la machine Centrifugeuse de laboratoire Maschinentyp 3 16K Type Type de la machine Bestell Nr 10280 10281 10282 10283 Part No Réf usine Normen EN 61010 2 020 Standards EN 61000 3 ...

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Page 7: ...3 System check 2 6 4 Ground wire check 2 6 5 Imbalance Monitoring System Installation and Start up 3 1 Unpacking of the centrifuge 3 1 1 Transport safety device 3 2 Installation 3 2 1 Site 3 2 2 Connection 3 2 3 Fuses emergency circuit breaker on site 3 3 Installation of rotors and accessories 3 3 1 Fastening of angle rotors with hermetically sealed lid 3 4 Initial start up 3 4 1 Switching on of t...

Page 8: ... Care and cleaning of centrifuge 6 2 Care and cleaning of accessories 6 3 Rotor pins 6 4 Glass breakage 6 5 Care and cleaning of the condenser 6 6 Sterilization and disinfection of rotor chamber and accessories 6 6 1 Autoclaving 6 7 Checks by operator 7 Appendix 7 1 Mathematical relations 7 1 1 Relative Centrifugal Force RCF 7 1 2 Density 7 2 Error correction 7 2 1 Centrifuge cannot be started 7 2...

Page 9: ...0 1000 23 031 9 970 Further parameters Time range Temperature setting range 0 30 min continuous run short time operation 10 to 40 C Dimensions Depth mm Width mm Height mm Weight kg EMC acc to EN 55011 Noise level dBA 600 630 355 78 Class B 65 Notes of user Serial number Supply date Inventory number Location Responsibility The figures are valid for an ambient temperature of 23 C 2 C and nominal vol...

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Page 11: ...17112 suitable for the system of rectangular carriers suitable for 11180 max radius 17 1 cm min radius 7 6 cm 4 500 3 871 17112 Sealing cap polysulfone clear incl 2 clips 17118 suitable for 13180 18000 Rectangular carrier undrilled for special tubes 85 110 mm long polyallomer 18002 Rectangular carrier for 20 reaction vials 1 5 2 2 ml max 11 mm e g 15008 15040 polypropylene 18003 Upper part for 180...

Page 12: ...llomer 18016 Rectangular carrier for 4 culture tubes 15 ml 15115 polypropylene 18017 Rectangular carrier for 10 tubes 15 ml and tubes with stopper max Ø 17 2 18 x 80 111 mm e g Monovettes 9 ml and 10 ml polypropylene 18025 Rectangular carrier for 5 glass tubes 25 ml max 24 x 85 105 mm e g 15025 15026 polyallomer 18022 Rectangular carrier for 4 sterilin tubes 30 ml graduated up to 20 ml with skirt ...

Page 13: ...0 polyallomer 18100 Rectangular carrier for 1 tube 100 ml max 45 5 48 x 85 110 mm e g 15100 15102 15103 15106 polyallomer 18105 Rectangular carrier for 20 RIA tubes 5 ml max 12 2 x 60 75 mm flat and round bottom tubes e g 15060 decantable polyallomer 18107 Rectangular carrier for 20 glass tubes 7 ml max 12 3 x 80 105 mm flat and round bottom tubes e g 15007 15027 decantable polyallomer 18115 Recta...

Page 14: ...able for 11180 max radius 17 2 cm min radius 7 5 cm 4 500 3 894 17190 Sealing cap polysulfone clear suitable for 13190 17350 Round carrier for 24 reaction vials 0 5 0 75 ml 8 10 x 28 31 mm e g 15005 polypropylene 17351 Round carrier for 12 Monovettes max 15 5 18 x 50 75 mm polypropylene 17352 Round carrier for 25 RIA tubes max 12 5 x 65 80 mm e g 15060 polypropylene 17353 Round carrier for 16 reac...

Page 15: ...15029 15030 15032 15033 polypropylene 17375 Round carrier for 3 tubes 50 ml max 35 38 x 90 110 mm e g 15049 15050 15056 polypropylene 17376 Round carrier for 4 tubes with screw cap 40 50 ml max 29 34 x 85 110 mm e g 15051 15052 15054 polypropylene 17346 Round carrier for 3 culture tubes 50 ml 15151 polypropylene 17385 Round carrier for 1 tube with screw cap 78 85 ml max 38 40 x 85 115 mm e g 13085...

Page 16: ...654 11133 Swing out rotor for 4 round buckets for the following accessories 5 500 5 445 13104 Round bucket aluminium sealable with caps 17109 17111 for the following round carriers and the 200 ml bottles 15202 and 15203 suitable for 11133 max radius 16 1 cm min radius 5 7 cm 5 500 5 445 17109 Sealing cap aluminium for hermetic sealing of bucket 13104 17111 Sealing cap polysulfone clear for hermeti...

Page 17: ...ylene 17015 Round carrier for 7 tubes 10 15 ml max 16 8 x 90 105 mm e g 15015 15020 15022 15023 15024 polyallomer 17018 Round carrier for 4 tubes with sealing cap 10 15 ml max 17 20 x 90 110 mm and Monovettes polyallomer 17019 Round carrier for 3 culture tubes 15 ml 15115 polypropylene suitable for 13104 without sealing cap 17025 Round carrier for 2 glass tubes 25 ml max 24 5 x 90 105 mm e g 15025...

Page 18: ...5100 15102 15103 15106 polypropylene 17125 Adapter for 1 bottle with screw cap 125 ml max 51 x 90 120 mm e g 15125 polypropylene 11132 Swing out rotor 28 x 15 ml complete con sisting of rotor 11133 4 round buckets 13117 4 carriers 17015 and 28 PS tubes 15020 max radius 14 9 cm min radius 5 7 cm 5 500 5 039 11224 Swing out rotor for cytology complete incl 4 buckets and carriers for different object...

Page 19: ...um lid max radius 8 2 cm min radius 5 cm angle 45 15 300 21 460 12131 Angle rotor 30 x 1 5 2 2 ml for reaction vials e g 15008 15040 incl hermetic aluminium lid max radius10 cm min radius 6 7 cm angle 45 14 000 21 913 12348 Angle rotor 48 x 1 5 2 2 ml for reaction vials e g 15008 15040 incl hermetic aluminium lid max radius 8 4 cm min radius 5 1 cm 15 300 21 984 12115 Angle rotor for 12 strips wit...

Page 20: ...us 8 4 cm min radius 2 1 cm angle 25 11 000 11 363 12156 Angle rotor 8 x 50 ml for sealed tubes 13055 plus 17054 15051 15052 15054 incl hermetic aluminium lid max radius 9 6 cm min radius 3 3 cm angle 25 10 000 10 733 19776 Angle rotor for 6 culture tubes 50 ml 15151 incl hermetic aluminium lid max radius 9 3 cm min radius 3 1 cm angle 25 9 500 9 384 13060 Adapter for 1 culture tube 15 ml 15115 su...

Page 21: ...er for 2 tubes 10 12 ml max 16 17 5 x 75 90 mm e g 15000 15010 15039 suitable for 12155 12159 polypropylene Adapters tubes and steel tubes 13000 Adapter POM for reaction vials 0 25 0 4 ml 15014 suitable for 12110 12131 12154 12348 14002 17008 18002 13002 Adapter POM for reaction vials 0 5 0 75 ml 15005 7 9 10 x 28 31 mm suitable for 12110 12131 12154 12348 14002 17008 18002 13021 Adapter for PCR t...

Page 22: ...steel sealing cap for 13026 15000 Teflon tube with screw cap 12 ml 16 1 x 81 1 mm suitable for 12111 12157 13084 17012 17355 18010 15010 ditto polycarbonate 15039 ditto polypropylene 15020 Polystyrene tube 15 ml 17 x 100 mm suitable for 17015 17358 18015 18115 15021 Polypropylene stopper for 15020 15023 15023 Polypropylene tube 15 ml 17 x 100 mm suitable for 17015 17358 18015 18115 15065 Plastic t...

Page 23: ...p for 13055 15051 Teflon tube with screw cap 42 ml 28 5 x 107 mm suitable for 12150 12156 13082 17052 17376 18051 15052 Polypropylene tube with screw cap 42 ml 28 8 x 107 mm suitable for 12150 12156 13082 17052 17376 18051 15054 Polycarbonate tube with screw cap 40 ml 28 8 x 107 mm suitable for 12150 12156 13082 17052 17376 18051 15048 Polyallomer tube with screw cap 42 ml 28 5 x 107 mm suitable f...

Page 24: ...159 17085 17385 18085 15102 Polypropylene tube 100 ml 45 x 100 mm suitable for 17101 17390 18100 15103 Polycarbonate tube 100 ml 45 x 100 mm graduated suitable for 17100 17101 17390 18100 15125 Polypropylene bottle with screw cap 125 ml 51 x 99 mm suitable for 17125 17395 18125 15202 Polypropylene bottle with screw cap 185 ml 56 5 x 113 mm suitable for 13104 13117 17400 18200 15203 Polycarbonate b...

Page 25: ...ditto graduated 15022 Special glass tube 15 ml 17 x 110 mm suitable for 12150 12156 with 16018 and for 12155 12159 with 16019 max permitted speed 7 000 rpm 15025 Glass tube 25 ml 24 x 100 mm suitable for 17025 17365 18025 15026 ditto graduated 15033 Special glass tube 30 ml 24 x 105 mm suitable for 12150 12156 with 16030 and for 12155 12159 with 16031 max permitted speed 7 000 rpm 15050 Glass tube...

Page 26: ...o for 18100 16909 Rubber plate for rectangular carriers 18005 18007 18015 16910 Rubber plate for round carriers 17006 17007 17012 17015 17025 16911 Rubber plate for round carriers 17050 17100 17112 Rectangular sealing cap polysulfone clear incl 2 clips 17118 for 13180 17111 Round sealing cap polysulfone clear for 13104 16251 Sealing ring 58 x 3 mm for 13104 17190 Round sealing cap polysulfone clea...

Page 27: ...e tubes e g glass tubes microtubes culture tubes Teflon tubes and especially high volume tubes can be used in our rotors buckets and adapters at speeds exceeding their breaking limit We recommend to always fill up the tubes and to follow the recommendations of the manufacturer ...

Page 28: ...Operating Manual SIGMA 3 16K page 28 of 69 03 06 ...

Page 29: ...art No 70104 1 tube of grease for load bearing bolts Part No 70284 Documentation Operating Manual Rotor and Accessories Operation and Use EU Statement of Conformity Equipment Decontamination Certificate Accessories according to your order our order confirmation and our delivery note Rotor Part No Rotor No 1 4 Standards and Regulations Please refer to the enclosed EU Statement of Conformity ...

Page 30: ...e centrifuge must be reduced see formula chapter 7 1 2 4 Operation of the centrifuge in hazardous locations is not allowed 5 During operation the centrifuge must not be moved Leaning against or resting on the centrifuge is not allowed 6 Do not spin explosive or highly inflammable materials 7 Substances which could damage the material of the centrifuge the rotors or the buckets anyhow must not be c...

Page 31: ...on consulter les documents joints Ein Netzverbindung On Power Marche mise sous tension Aus Netzverbindung Off Power Arrêt mise hors tension Schutzleiteranschluß Protective earth ground Liaison à la terre Erde Earth ground Terre Netzstecker ziehen Unplug mains plug Tirer la fiche de prise Vorsicht Quetschgefahr Caution Risk of bruising Attention Danger de blessure Drehrichtungspfeil Arrow direction...

Page 32: ... lid is secured by solid hinges and at the front by a cover lock 2 3 Drive The drive motor is a well dimensioned asynchronous motor 2 4 Operation and Display The grafical LCD display is hermetically sealed operation is executed via two knobs Any operating status is indicated 2 5 Electronics The electronics controlled by a microprocessor allows extensive adaptations of the centrifuge to the differe...

Page 33: ...ing of the centrifuge lid may only be possible if the rotor is at standstill This standstill is checked by the microprocessor 2 6 3 System Check An internal system check monitors data transmission and the sensor signals with regard to plausibility In case of a malfunction an error message is displayed under a number in the Speed and rcf display 2 6 4 Ground Wire Check For ground wire check there i...

Page 34: ... device or with several persons When lifting or carrying the centrifuge please always reach under the instrument from the side Attention The instrument is heavy Please keep case for possible transport of centrifuge later 3 1 1 Transport Safety Device made of foamed material The SIGMA 3 16K has a transport safety device between the rotor chamber and the motor It is accessible after opening of the c...

Page 35: ...oltage on the name plate must correspond to the local supply voltage SIGMA laboratory centrifuges are units of safety class I DIN VDE 0700 and in clude a three wire power cord 2 5 m long with shockproof right angle plug The instrument has thermal fuses In case of a disconnection through the thermal fuses allow a cool down phase of two minutes after which they could be reactivated by a switch At th...

Page 36: ...ositions of swing out rotors with buckets Make sure that all buckets are inserted correctly 6 Use only appropriate vessels for the rotor please refer to chapter 1 2 Suitable acces sories as well Universal grease allowed not allowed 7 Fill vessels external to the centrifuge 8 Put or screw on covers of vessels 9 Opposite places of the rotors must always be loaded with same accessories and same filli...

Page 37: ...onto rotor and tighten it 2 Lower rotor with lid 2 onto motor shaft 3 Put rotor tie down screw 1 onto motor shaft and tighten using the wrench 4 The rotor can be run without lid 2 as well 5 The rotor and lid seals 3 must be greased after cleaning 6 Special instructions for the use of hermetically sealed rotors All rotors can be installed or removed with closed lid after loosening the rotor tie dow...

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Page 39: ...Switching on of the Centrifuge Press mains switch on the right side panel The centrifuge display is illuminated 3 4 2 Opening of the Lid Press the lid key The lid opens 3 4 3 Installation of a Rotor Put a rotor onto the shaft and fasten it by screwing the rotor tie down screw clockwise onto the drive shaft Please use the supplied rotor wrench refer to chapter 3 3 Installation of rotors and accesso...

Page 40: ...GMA 3 16K page 40 of 69 03 06 4 1 Operating Panel The centrifuge is operated via the operating panel Keys can be pressed when their LED is on When power is applied all LEDs and the display are illuminated for a short time ...

Page 41: ...n the display is flashing Cancellation of this function occurs in the same way 4 1 2 Stop key with Preselection of the Soft Stop and or Soft Start Function This key can be used for the following early terminating the run The centrifuge decelerates to a complete stop Deceleration can be terminated by pressing the start key again and the centrifuge can be restarted activating the soft stop and or so...

Page 42: ...d has to be opened again When closing the lid both locks must catch 4 1 4 Knobs The parameters are set by turning the knobs Left knob Activation of set by selecting speed rcf time temperature rotor or lock Remark set is automatically deactivated after 60 seconds if no further changes are made the lock symbol appears Thereafter changes are impossible Right knob Preselection of the desired set value...

Page 43: ...pletely active The display consists of the following areas Time Symbol lock Temperature Speed RCF Rotor Imbalance run mode 4 2 1 Speed RCF Relative Centrifugal Force Rotor dimensions and speed determine the RCF value Entry of one value automati cally determines the other one ...

Page 44: ...lue can be changed during the run by turning the right knob 4 2 1 2 Relative Centrifugal Force RCF The relative centrifugal force RCF is the acceleration which the sample is exposed to The display rcf shows the rcf value Activate set in the rcf area with the left knob The rcf value can be changed by turning the right knob This is also possible when the centrifuge is running Preselection of 1 x g b...

Page 45: ...hows the total set time the remaining run time or the elapsed time The time value as well as different time modes can be set by turning the right knob If the time is changed during centrifugation the total newly entered period is run the time elapsed before is not considered ...

Page 46: ...ight knob the display shows min This total run time max 30 minutes is displayed by a sequence of bars before starting the centrifuge During centrifugation this time is counted down the sequence of bars indicates the remaining run time The set time is indicated by one single bar The centrifuge run can be early terminated by pressing the stop key The set time and the remaining run time remain on the...

Page 47: ...uous run Unlike the standard run mode the minutes are counted up A sequence of bars indicates the elapsed run time One single bar at is indicating the continuous run mode After 30 minutes the remaining run time is no longer displayed but the run continues The continuous run is terminated by pressing the stop key and the centrifuge decelerates to a complete stop The elapsed time is displayed in min...

Page 48: ... After release of the start key the centrifuge decelerates with maximum power to standstill During short run the time is counted up in seconds sec is displayed A sequence of bars is indicating the elapsed run time One single bar at short run indicates that short time operation had been selected After termination of the short run the elapsed run time in seconds remains on the display ...

Page 49: ...he short run the time is counted up in seconds A sequence of bars indicates the elapsed run time One single bar at short run indicates that short time operation had been selected After 30 seconds the centrifuge decelerates to a complete stop The short run can be early terminated by pressing the stop key The centrifuge decelerates to standstill The elapsed run time in seconds remains on the display...

Page 50: ...ble in steps of 1 C Temperatures between 10 C and 40 C can be pre selected If the set temperature is lower than the actual temperature the set value is indicated by a flashing bar As soon as the lid is opened the cooling system is disconnected in order to avoid icing of the rotor chamber Precooling of the rotor below 0 C during standstil l is not recommended Differences between actual and displaye...

Page 51: ... using the left knob An other rotor can be selected by turning the right knob This is not possible during a centrifuge run Attention An automatic rotor identification occurs after the centrifuge has been started If the used rotor is not the same than the preselected one the used rotor is displayed and the preselected speed is corrected Preselected rotor 12158 Changed to rotor 12150 ...

Page 52: ... only The vessels must be loaded symmetrically so that the buckets and their suspensions are loaded evenly Loading an eg only an outer position of the bucket is not allowed 8 1Centrifugation with different tubes Working with different tube sizes is possible Opposite places must be loaded with the same vessels The example shows a swing out rotor with buckets and 2 x carriers 100 ml and 2 x carriers...

Page 53: ...n increased wear of bearings 13 Use perfect accessories only 14 Avoid corrosion to accessories by careful maintenance 15 Spin infectious material in sealed rotors and buckets only 16 Do not spin explosive or highly imflammable materials 17 When centrifuging substances with a density 1 2 g cm3 the allowable max speed must be reduced refer to chapter 7 1 2 Density 18 Grease joints of buckets and rot...

Page 54: ...s of max homogeneous density of 1 2 g cm3 if centrifuged at max speed If liquids of higher density are used the speed must be reduced accordingly refer to chapter 7 1 Mathematical relations 7 Operation with rotors and adapters showing corrosion or other defects 8 Operation of very corrosive substances which can cause damages to material and affect mechanical strength of rotors and adapters 9 Opera...

Page 55: ...rotor is spinning 17 Such materials are prohibited which chemically interact vigorously 18 Do not spin explosive or inflammable materials 19 Substances which could damage the material of the centrifuge the rotors or the adapters must not be centrifuged Infectious toxic pathogene or radioactive substances must be centrifuged in certified rotors and vessels only and all necessary safety precautions ...

Page 56: ...n the material without visible signs When detecting a visible damage of the surface a crack a mark or any other change also corrosion the part rotor etc must be replaced immediately In order to avoid corrosion rotor incl tie down screw and cover seal and adapters must be cleaned and greased regularly with the supplied slushing oil Sigma part no 70104 for 20 ml slushing oil Before using detergents ...

Page 57: ...and the metal dust from the rotor chamber it is advisable to grease the upper part of the centrifuge chamber with e g Vaseline Then the rotor should rotate for some minutes at a moderate speed The glass and metal particles will now collect at the greased part and can easily be removed with a cloth together with the grease If necessary repeat this procedure 6 5 Care and Cleaning of the Condenser A ...

Page 58: ... recommended by us the user has to contact us to make sure that such procedure would not damage the centrifuge For sterilization by steam resistance to temperature of the individual material must be checked refer to point 6 6 1 Autoclaving Please contact your laboratory safety officer regarding proper methods to use If dangerous materials are used the centrifuge and the accessories must be disinfe...

Page 59: ...40 20 Polypropylene tubes 115 118 30 40 30 Teflon tubes 134 138 3 5 100 Aluminium rotors 134 138 3 5 Polypropylene rotor 12034 115 118 30 40 20 Polypropylene rotor 12124 115 118 30 40 20 Polycarbonate Polyallomer lids for angle rotors 115 118 30 40 20 Polysulfone lids for angle rotors 134 138 3 5 100 Aluminium buckets 134 138 3 5 Polycarbonate caps for buckets 115 118 30 40 50 Polypropylene caps f...

Page 60: ...tor suspension 2 Concentricity of the motor shaft 3 Fastening of the trunnions in the rotor 4 Rotors and accessories have a limited life For safety reasons a regular check is recommended after 50 000 cycles Any changes like e g corrosion cracks material abrasion etc require special attention 5 Screw connections have to be tight Furthermore the earth wire must be checked regularly ...

Page 61: ... third value is determined by the equation If the speed or the rotation radius is changed the resulting RCF will be recalculated If the RCF is changed the speed under consideration of the radius is adapted accordingly r radius in cm n speed in rpm RCF without dimension 7 1 2 Density If the density of the liquid is higher than 1 2 g cm3 the allowed maximum speed of the centrifuge is reduced accordi...

Page 62: ...tch on power Close lid please refer to chapter 7 2 3 Lid cannot be opened closed 7 2 1 Centrifuge cannot be Started Start key LED illuminated Power off on If error occurs again call service Lid key LED flashing Open and close the lid again If error occurs again although the lock engaged call service 7 2 2 Centrifuge Decelerates during Operation Centrifuge displays an error 1 to 11 after power on P...

Page 63: ...ts 7 3 Error Mode In the error mode the Start Stop and Lid keys are flashing ERROR is displayed in the speed area and the error code in the rcf area 7 3 1 Error Codes Error no Kind of error Actions 1 Tacho signal disturbed Power off on 4 Lid does not open after pressing the Lid key Power off on Emergency lid release 2 3 5 11 17 30 Internal fault Power off on 12 Imbalance determined Remove imbalanc...

Page 64: ...n The following declarations serve for keeping safety and health of our employees Fill in the forms and attach them when returning centrifuges accessories and spare parts Please understand that we cannot carry out any work before we have the declarations We recommend to make several copies of this page ...

Page 65: ...ome copies before removing this page Attention This form must be glued on outside of the box Return declaration YES NO Decontamination declaration inside Unit component contaminated Unit component unused new Attention This form must be glued on outside of the box ...

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Page 67: ..._____________________________________________________ with gloves only _________________________________________________________________ General characteristics of the substances Corrosive Ο Explosive Ο Biologically hazardous Ο Radioactive Ο Toxic Ο In combination with which substances may hazardous mixtures develop Name of the substances Has the equipment been cleaned before shipment YES Ο NO Ο I...

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