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Sigma 2-7, Operating Manual

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Laboratory centrifuge Sigma 2-7, Sigma 2-7 Cyto 

Laboratory centrifuge Sigma 2-7 IVD 

7 Malfunctions and error correction 

 

 

 

Version 07/2015, Rev. 1.12 of 16/07/2019 

• sb 

37 / 68 

Translation of the original operating manual, part no. 0702802 

 

 

7 

Malfunctions and error correction 

 

 

7.1 

General malfunctions 

 Malfunctions are indicated by error messages with a number in the 
speed/RCF display. In addition, the blue LED flashes rapidly. 

In the event of a fatal error (e.g. a defective lid lock), a certain safety time 
will be counted down on the display. During this time, ―ERR‖ and ―SAFE‖ 
flash alternately on the display. When the time is up, "OFF" will be 
displayed. 

 

 

WARNING 

Do not switch the centrifuge off until ―OFF‖ is displayed! This is necessary 
in order to ensure that the rotor is at a complete standstill. 

 

 

 

 
•  Eliminate the source of the problem (see tables below). 
•  Acknowledge the error messages with the lid key. 
 

 

 

Type of error 

Possible reason 

Correction 

No indication on the display 

No power in the mains supply 

Check fuse in the mains supply 

Power cord is not plugged in 

Plug in power cord correctly 

Mains power switch off 

Switch mains power switch on 

Lid is not closed correctly 

Close the lid 

Centrifuge cannot be started:  The set 
speed value is displayed in an 
unchanged manner 

Several possible causes 

Power off/on. If the error occurs 
again, contact service 

The lid lock is not closed correctly  

Open and close lid. If the error 
occurs again, contact service 

Centrifuge decelerates during 
operation and displays an error from 1 
to 18 after powering on  

Several possible causes 

Power off/on. If the error occurs 
again, contact service 

Centrifuge decelerates during 
operation and displays error 19 after 
powering on  

Several possible causes 

Acknowledge by pressing the lid key 

Lid cannot be opened 

Lid locks have not released 

Unlock the lid manually (see chapter 
7.1.1 - "Emergency lid release")and 
contact service 

Lid seal sticks 

Clean the lid seal and apply talcum 
powder 

Temperature value cannot be reached 
(only for refrigerated centrifuges) 

Condenser dirty 

Contact service 

 

 

 

Summary of Contents for 2-7

Page 1: ...15 Rev 1 12 of 16 07 2019 Sigma 2 7 Sigma 2 7 Cyto Laboratory centrifuge Sigma 2 7 IVD Laboratory centrifuge part no 0702802 Sigma 2 7 Sigma 2 7 Cyto Sigma 2 7 IVD from serial no 157336 Laboratory cen...

Page 2: ...Laboratory centrifuge Sigma 2 7 Sigma 2 7 Cyto Laboratory centrifuge Sigma 2 7 IVD 2 68 Version 07 2015 Rev 1 12 of 16 07 2019 sb Translation of the original operating manual part no 0702802...

Page 3: ...015 Rev 1 12 of 16 07 2019 sb 3 68 Translation of the original operating manual part no 0702802 Copyright by Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode am Harz Germany Tel 49 0...

Page 4: ...Laboratory centrifuge Sigma 2 7 Sigma 2 7 Cyto Laboratory centrifuge Sigma 2 7 IVD 4 68 Version 07 2015 Rev 1 12 of 16 07 2019 sb Translation of the original operating manual part no 0702802...

Page 5: ...Area of application 13 2 2 2 1 Speed radius and relative centrifugal force 14 2 2 2 2 Density 14 3 Safety 15 3 1 Marking of the unit 15 3 2 Explanation of the symbols and notes 16 3 3 Responsibility o...

Page 6: ...f a rotor 27 6 2 2 2 Installation of accessories 28 6 2 2 3 Adapters 29 6 2 2 4 Vessels 29 6 3 Control System Spincontrol Basic 30 6 3 1 User interface 30 6 3 2 Display 30 6 3 3 Starting a centrifugat...

Page 7: ...s 42 8 1 3 Rotors buckets and carriers 43 8 1 4 Glass breakage 43 8 2 Sterilisation and disinfection of the rotor chamber and accessories 44 8 2 1 Autoclaving 45 8 3 Service 46 8 4 Return of defective...

Page 8: ...ratory centrifuge Sigma 2 7 Sigma 2 7 Cyto Laboratory centrifuge Sigma 2 7 IVD Table of contents 8 68 Version 07 2015 Rev 1 12 of 16 07 2019 sb Translation of the original operating manual part no 070...

Page 9: ...xtures with a maximum density of 1 2 g cm3 The laboratory centrifuge that is marked with IVD is intended for human biological samples including donated blood and tissue in conjunction with diagnostic...

Page 10: ...d information that must not be duplicated distributed or communicated in any other way Non compliance may be prosecuted under criminal law 1 5 Standards and regulations EC declaration of conformity se...

Page 11: ...original operating manual part no 0702802 2 Layout and mode of operation 2 1 Layout of the centrifuge 2 1 1 Functional and operating elements 1 Lid 2 Display 3 User interface see chapter 6 3 1 User in...

Page 12: ...rt no 0702802 2 1 2 Name plate 1 Manufacturer 2 Power consumption 3 Max speed 4 Max kinetic energy 5 Serial number 6 Part number 7 Nominal voltage 8 Type 9 Input fuse 10 CE mark in compliance with the...

Page 13: ...adratically as a function of the angular velocity The bigger the radius in the rotor chamber is and the higher the speed is the higher the centripetal acceleration is However the forces acting on the...

Page 14: ...way of the stated formula If afterwards the speed or the radius is changed the resulting relative centrifugal force will be recalculated automatically by the control unit If the RCF is changed the sp...

Page 15: ...late see chapter 2 1 2 Name plate Protective earth ground CE mark in compliance with the directive 2006 42 EC Earth ground Do not dispose as part of domestic waste Unplug the mains plug Do not dispose...

Page 16: ...stands for a direct hazard to the life and health of persons Non observance of these symbols causes serious health problems up to life endangering injuries DANGER This symbol stands for a direct hazar...

Page 17: ...he centrifuges The operator must perform a risk assessment concerning potential accidents in connection with the centrifuge and take design related countermeasures if necessary The centrifuge has to b...

Page 18: ...tor is in motion Do not reach into the rotor chamber when the rotor is in motion Do not use the centrifuge if it was installed incorrectly Do not use the centrifuge without panels Do not use the centr...

Page 19: ...enic toxic or radioactive samples are intended to be used in the centrifuge it is in the responsibility of the user to ensure that all necessary safety regulations guidelines precautions and practices...

Page 20: ...We explicitly warn against the use of equipment of poor quality Breaking glass or bursting vessels can cause dangerous imbalances at high speeds Ensure that rotor and buckets are correctly fitted see...

Page 21: ...ycles and a rotor with the engraving Exp date 02 20 must be scrapped in February 2020 at the latest see figure If a specification concerning the maximum number of cycles and a specification concerning...

Page 22: ...check An earth conductor check can be carried out by authorised and specialised personnel using a suitable measuring instrument Please contact the Sigma service department see chapter 7 3 Service con...

Page 23: ...ht 23 kg 4 2 Storage conditions The centrifuge can be stored in its original packaging for up to a year Store the centrifuge only in dry rooms The permissible storage temperature is between 20 C and 6...

Page 24: ...is packaged in a cardboard box Open the box Take out the box containing the accessories Remove the upper foam element Lift the centrifuge out of the cardboard box When lifting the centrifuge always re...

Page 25: ...st be stable and have a solid even surface Attention During transport from cold to warmer places condensational water will collect inside the centrifuge It is important to allow sufficient time for dr...

Page 26: ...d installed see chapter 5 Set up and connection 6 2 Switching the centrifuge on Press the mains power switch The display then illuminates The centrifuge is ready for operation 6 2 1 Opening and closin...

Page 27: ...bore straight down onto the motor shaft Tighten the rotor tie down screw clockwise with the supplied rotor wrench with 3 Nm In doing so hold the rotor at its outer rim Follow the safety instructions...

Page 28: ...es and fill to avoid imbalance Centrifugation with different tube sizes Working with different tube sizes is possible In this case however it is very important that axial symmetrical inserts are ident...

Page 29: ...in order to avoid imbalance If all of the compartments of a carrier are not used the buckets must be loaded evenly Loading the edges of a bucket only is not permissible 6 2 2 4 Vessels General Load t...

Page 30: ...3 Program key 4 Arrow keys 5 Lid key 6 Start Stop key 7 Quick run key Fig 8 User interface example The centrifuge is started directly via the user interface When the centrifuge is switched on all seg...

Page 31: ...and to restart the centrifuge 6 3 6 Speed Relative centrifugal force RCF The RCF value is determined by the rotor geometry and speed The RCF and speed values therefore depend on each other If one of t...

Page 32: ...ted immediately NOTE If the centrifugation time is changed during the run the centrifuge will run for the entire new time and will disregard the previous runtime that has already elapsed 6 3 7 2 Short...

Page 33: ...e Set button until the rotor selection screen is displayed Then release the Set button Use the arrow buttons to select the ID number code of the rotor that is used from the table see below Press the s...

Page 34: ...ning function The automatic lid opening function ensures that the lid opens when the rotor has stopped In the factory settings the automatic lid opening function is activated To deactivate the automat...

Page 35: ...e sound signal is deactivated Proceed in the same manner in order to activate the sound signal 6 3 13 Button signal This function provides a sound signal that is issued whenever a button is pressed In...

Page 36: ...il the corresponding unit flashes on the display Select the desired parameters In order to save the data start the centrifuge or press the set key repeatedly until the indication Prog stops flashing N...

Page 37: ...tion No indication on the display No power in the mains supply Check fuse in the mains supply Power cord is not plugged in Plug in power cord correctly Mains power switch off Switch mains power switch...

Page 38: ...d from the socket Remove the plug see figure item 1 from the front panel e g with a screwdriver Fig 10 Position of the opening for the emergency lid release Insert the supplied hexagon socket key hori...

Page 39: ...r off Allow to cool down Provide better ventilation only air cooled centrifuges Provide sufficient water throughput only water cooled centrifuges 46 49 Imbalance error only for centrifuges with imbala...

Page 40: ...n the event of queries malfunctions or spare part enquiries From Germany Contact Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode Germany Tel 49 0 55 22 50 07 44 44 E mail support lab...

Page 41: ...and aggressive substances Do not use solvents Do not use agents with abrasive particles Do not expose the centrifuge and rotors to intensive UV radiation or thermal stress e g by heat generators 8 1...

Page 42: ...once a week or preferably after each use Adapters should be removed cleaned and dried CAUTION Do not clean the accessories in a dishwasher Cleaning in a dishwasher removes the anodised coating the res...

Page 43: ...with a cloth so that it forms a thin layer 8 1 4 Glass breakage CAUTION In the case of glass breakage immediately remove all glass particles e g with a vacuum cleaner Replace the rubber cushions sinc...

Page 44: ...r drugstores The centrifuge and the accessories consist of various materials A possible incompatibility must be considered Before using cleaning or decontamination agents that were not recommended by...

Page 45: ...e min Max time min Max cycles Aluminium buckets 134 138 3 5 Aluminium rotors 134 138 3 5 Glass tubes 134 138 3 40 Polyallomer polycarbonate rectangular carriers 115 118 30 40 Polyallomer polycarbonate...

Page 46: ...heless wear cannot be excluded and it may not be visible from the outside Especially the rubber parts that are among other things part of the motor suspension are subject to ageing This is why we reco...

Page 47: ...r this purpose we require a declaration of decontamination The form must be filled in completely and signed by authorised and specialised personnel only Affix the original form in a clearly visible ma...

Page 48: ...ating manual part no 0702802 NOTE The defective part unit must be packaged in a transport safe manner Please use the original packaging for the unit if at all possible If the product is dispatched to...

Page 49: ...ol means that it is not permissible to dispose of the unit among household waste You can return these centrifuges free of cost to Sigma Laborzentrifugen GmbH Ensure that the unit is decontaminated Fil...

Page 50: ...Electrical connection Protection class IP code see name plate I 20 Max connected load kVA Max power consumption kW Max current consumption A Input fuse AF 0 26 at 100 240 V 50 60 Hz 0 21 at 100 240 V...

Page 51: ...ield x g Max kinetic energy Nm 400 2 000 400 635 1 821 Other parameters Time range min 1 99 short run continuous run Physical data Height mm Height with opened lid mm Width mm Depth mm Weight kg 293 6...

Page 52: ...sories The complete list of accessories can be downloaded from www sigma zentrifugen de 11 1 1 Rotor radii The information in the accessories table concerning the radius refers to the values of the re...

Page 53: ...Cyto Laboratory centrifuge Sigma 2 7 IVD 11 Appendix Version 07 2015 Rev 1 12 of 16 07 2019 sb 53 68 Translation of the original operating manual part no 0702802 11 2 Speed gravitational field diagram...

Page 54: ...ition 1 16K Edition 12082 7 years 1 14 1 14K 12083 7 years 1 14 1 14K 12084 7 years 1 14 1 14K 12085 7 years 1 14 1 14K 12092 5 years 20x 1 14 1 14K 12093 5 years 20x 1 14 1 14K 12094 5 years 20x 1 14...

Page 55: ...ile C3H3N 100 1 1 4 3 3 4 4 4 1 4 1 Allyl alcohol C3H6O 96 1 3 3 2 2 2 2 4 1 1 1 Aluminium chloride AlCl3 saturated 1 3 2 4 1 1 1 1 4 Aluminium sulfate Al2 SO4 3 10 1 1 1 3 1 1 1 1 1 1 1 Ammonium chlo...

Page 56: ...2 1 3 1 2 1 Diaminoethane C2H8N2 100 1 1 3 1 1 3 4 1 1 1 Diesel fuel 100 1 1 3 1 1 1 3 1 1 1 Dimethyl formamide DMF C3D7NO 100 1 1 4 1 1 4 3 1 3 1 Dimethyl sulfoxide DMSO C2H6SO 100 1 2 4 1 1 4 4 1 1...

Page 57: ...CH4O 100 1 2 4 1 1 3 1 3 1 2 1 Methyl benzene C7H8 100 3 1 4 1 3 4 4 4 1 4 1 Methyl ethyl ketone MEK C4H8O 100 1 1 4 1 1 4 4 4 1 4 1 Methylene chloride CH2Cl2 100 4 3 4 3 3 4 4 4 1 1 Mineral oil 100...

Page 58: ...Cl 30 1 1 1 1 1 1 1 1 1 1 3 Sodium hydroxide NaOH 30 1 1 4 1 1 1 1 1 1 2 4 Sodium hydroxide NaOH 50 1 1 4 1 1 1 1 1 2 4 Sodium sulfate Na2SO4 10 1 1 1 1 1 1 1 1 1 1 1 Spirits C2H6O 96 1 1 1 1 1 1 1 3...

Page 59: ...ge Sigma 2 7 Sigma 2 7 Cyto Laboratory centrifuge Sigma 2 7 IVD 11 Appendix Version 07 2015 Rev 1 12 of 16 07 2019 sb 59 68 Translation of the original operating manual part no 0702802 11 5 EC declara...

Page 60: ...boratory centrifuge Sigma 2 7 Sigma 2 7 Cyto Laboratory centrifuge Sigma 2 7 IVD 11 Appendix 60 68 Version 07 2015 Rev 1 12 of 16 07 2019 sb Translation of the original operating manual part no 070280...

Page 61: ...boratory centrifuge Sigma 2 7 Sigma 2 7 Cyto Laboratory centrifuge Sigma 2 7 IVD 11 Appendix Version 07 2015 Rev 1 12 of 16 07 2019 sb 61 68 Translation of the original operating manual part no 070280...

Page 62: ...boratory centrifuge Sigma 2 7 Sigma 2 7 Cyto Laboratory centrifuge Sigma 2 7 IVD 11 Appendix 62 68 Version 07 2015 Rev 1 12 of 16 07 2019 sb Translation of the original operating manual part no 070280...

Page 63: ...gma 2 7 Sigma 2 7 Cyto Laboratory centrifuge Sigma 2 7 IVD 11 Appendix Version 07 2015 Rev 1 12 of 16 07 2019 sb 63 68 Translation of the original operating manual part no 0702802 11 6 Declaration of...

Page 64: ...boratory centrifuge Sigma 2 7 Sigma 2 7 Cyto Laboratory centrifuge Sigma 2 7 IVD 11 Appendix 64 68 Version 07 2015 Rev 1 12 of 16 07 2019 sb Translation of the original operating manual part no 070280...

Page 65: ...n colour 45 Changes in structure 45 Changing the runtime during centrifugation 32 Changing the speed RCF value during centrifugation 31 Chemical and biological safety 19 Chemical resistance of plastic...

Page 66: ...mable substances 19 Informal safety instructions 17 Initial start up 26 Input fuse 12 50 51 Inspection by the manufacturer 46 Installation of accessories 28 Installation of rotors and accessories 27 I...

Page 67: ...r tie down screw 43 Rotors and accessories with a different service life 21 54 Rotors cleaning and care 43 Runtime 32 S Safety area 20 Safety devices 21 Safety distance 18 20 25 Safety instructions 9...

Page 68: ...21 54 Technical data 50 51 Technical documentation 51 Temperature error 39 Temperature value cannot be reached 37 Thermal stress 41 Time range 50 51 Toxic substances 19 41 Transport safety device 24...

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