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Refrigerated Centrifuge Sigma 2-16KL

11 Appendix

Version 08/2012, Rev. 1.24 of 28/04/2022

• sb

67 / 82

Translation of the original operating manual, part no. 0700702

Pos : 221 /100 Sigma/100 BA Z entrif ugen Sigma (St andardm odul e)/110 Anhang/ 110-0020 Dr ehz ahl-Sc hwer ef eld-Diagr amm @ 28\m od_1405320705642_68. docx @ 195153 @ 2 @ 1

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11.2 Speed-gravitational-field-diagram

Fig. 33: Speed-gravitational-field-diagram

Pos : 222 /010 Univ ers almodul e/ Seit enwechs el @ 0\mod_1202116244312_0.docx @ 105 @ @ 1

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Summary of Contents for 2-16KL

Page 1: ...ation of the original operating manual sb 08 2012 Rev 1 24 of 28 04 2022 Sigma 2 16KL part no 0700702 Sigma 2 16KL from serial no 146109 Refrigerated Centrifuge Operating Manual Please retain for late...

Page 2: ...Refrigerated Centrifuge Sigma 2 16KL 2 82 Version 08 2012 Rev 1 24 of 28 04 2022 sb Translation of the original operating manual part no 0700702...

Page 3: ...022 sb 3 82 Translation of the original operating manual part no 0700702 Copyright by Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode am Harz Germany Tel 49 0 5522 5007 0 Fax 49 0 55...

Page 4: ...Refrigerated Centrifuge Sigma 2 16KL 4 82 Version 08 2012 Rev 1 24 of 28 04 2022 sb Translation of the original operating manual part no 0700702...

Page 5: ...gal force 14 2 2 2 2 Density 14 3 Safety 15 3 1 Marking of the unit 15 3 2 Explanation of the symbols and notes 16 3 3 Responsibility of the operator 17 3 4 Requirements concerning the personnel 18 3...

Page 6: ...gle rotor with a hermetic lid 31 6 2 2 3 Installation of the microhaematocrit rotor 32 6 2 2 4 Installation of accessories 33 6 2 2 5 Adapters 35 6 2 2 6 Vessels 35 6 3 Control system Spincontrol L 36...

Page 7: ...carriers 55 8 1 4 Microhaematocrit rotor 55 8 1 5 Load bearing bolts 56 8 1 6 Glass breakage 57 8 2 Sterilisation and disinfection of the rotor chamber and accessories 57 8 2 1 Autoclaving 58 8 3 Serv...

Page 8: ...Refrigerated Centrifuge Sigma 2 16KL Table of contents 8 82 Version 08 2012 Rev 1 24 of 28 04 2022 sb Translation of the original operating manual part no 0700702...

Page 9: ...odule 010 Allgem eine Inform ationen 010 0020 Bestimm ungsgem e Verw endung Sigma 26 m od_1405318951312_68 docx 191709 2 1 1 2 Intended use Centrifuges are power driven machines that separate liquids...

Page 10: ...N ormen und Vorschriften 26 mod_1405318958202_68 docx 191765 2 1 1 5 Standards and regulations These operating instructions have been created in accordance with the relevant European standards and re...

Page 11: ...ufbau und Wirkungsw eise 020 0010 Aufbau der Zentrifuge 26 m od_1405318982945_68 docx 191793 2 1 2 1 Layout of the centrifuge Pos 17 100 Sigma 110 BA Zentrifugen Sigma Pr ojekte 2 16KL_2 16KH L 020 Au...

Page 12: ...2 16KL 29 m od_1405321347233_68 docx 196609 3 1 2 1 2 Name plate 1 Manufacturer 2 Power consumption 3 Max speed 4 Max kinetic energy 5 Serial number 6 Part number 7 Type 8 Nominal voltage 9 CE mark in...

Page 13: ...bigger the radius in the rotor chamber is and the higher the speed is the higher the centripetal acceleration is However the forces acting on the rotor also increase Pos 23 010 U niversalmodule Leerz...

Page 14: ...force will be recalculated automatically by the control unit If the RCF is changed the speed will be adapted while the specified radius is maintained The speed gravitational field diagram provides an...

Page 15: ...fuge I On Power Arrow indicating the direction of rotation 0 Off Power Rotor loading information see chapter 2 1 2 Name plate Name plate see chapter 6 2 2 4 Installation of accessories CE mark in comp...

Page 16: ...e of these symbols causes serious health problems up to life endangering injuries DANGER This symbol stands for a direct hazard to the life and health of persons due to electrical voltage Non observan...

Page 17: ...l health and safety regulations as well as with the accident prevention regulations must be checked at regular intervals e g every month Under the international rules for health and safety at work the...

Page 18: ...ised persons are present in the danger zone or work area This manual uses the following personnel qualifications for various areas of activity Qualified electrician Due to their special training knowl...

Page 19: ...the location of use of the centrifuge Ensure that it is accessible at all times The operating manual must be handed over to any subsequent owner or operator of the centrifuge Any changes additions or...

Page 20: ...ich the housing needs to be removed Inspect the electrical equipment of the unit regularly Defects such as loose or burnt cables must be eliminated immediately Following the completion of any type of...

Page 21: ...319025698_68 docx 192073 3 1 3 6 3 Fire prevention DANGER Do not spin explosive or inflammable substances Do not use the centrifuge within hazardous locations Pos 49 010 U niversalmodule Leerzeile 0 m...

Page 22: ...ssels can cause dangerous imbalances at high speeds Ensure that rotor and buckets are correctly fitted see chapter 6 2 2 1 Installation of a rotor Observe the instructions on the installation of acces...

Page 23: ...n etc After 10 years they must be inspected by the manufacturer After 50 000 cycles the rotor must be scrapped for reasons of safety If other data concerning the service life are engraved on the rotor...

Page 24: ...number see chapter 7 2 Table of error codes Pos 65 010 U niversalmodule Leerzeile 8pt 12 mod_1337063062114_0 docx 65687 1 Pos 66 100 Sigma 100 BA Zentrifugen Sigma Standar dmodule 030 Sicher heit 030...

Page 25: ...tandar dmodule 030 Sicher heit 030 0100 R estrisiken 27 mod_1405319052885_68 docx 192339 2 1 3 9 Remaining hazards The centrifuge was built in accordance with the state of the art and in compliance wi...

Page 26: ...4 2 Storage conditions The centrifuge can be stored in its original packaging for up to a year Store the centrifuge only in dry rooms The permissible storage temperature is between 20 C and 60 C If y...

Page 27: ...1 Pos 88 100 Sigma 110 BA Zentrifugen Sigma Pr ojekte 2 16KL_2 16KH L 040 Lagerung und Tr ansport 040 0050 Transportsicherung 2 16KL_KH L berschrift 29 m od_1405321382496_68 docx 196679 2 1 4 5 Transp...

Page 28: ...be stable and have a solid even surface Attention During transport from cold to warmer places condensational water will collect inside the centrifuge It is important to allow sufficient time for dryi...

Page 29: ...ule Leerzeile 0 m od_1202116244500_0 docx 114 1 Pos 98 100 Sigma 100 BA Zentrifugen Sigma Standar dmodule 050 Aufstellung und Anschl uss 050 0020 0020 Sicherungen bauseits au er 3 30KS 8KS 8KBS 7 m od...

Page 30: ...he lid The lid can be opened if the centrifuge is at a standstill and if the lid key is illuminated Press the lid key in order to open the lid The centrifuge cannot be started if the lid is opened To...

Page 31: ...r ol L S 060 0020 0020 0021 Einsetzen eines Wi nkelrotors mit H ermetikdeckel 3 Nm 29 mod_1405321531516_68 docx 197015 4 1 6 2 2 2 Installation of an angle rotor with a hermetic lid Open the centrifug...

Page 32: ...63 4 1 6 2 2 3 Installation of the microhaematocrit rotor Open the centrifuge lid by pressing the lid key Replace the rotor tie down screw with the rotor tie down screw of the microhaematocrit rotor p...

Page 33: ...oint in the capillary tubes You can now read the percentage value Remove the capillary tubes Some of the tubes can also be evaluated with the reader outside of the rotor see the instructions for use o...

Page 34: ...enly It is not permissible to load angle rotors on only one axis correct incorrect Fig 7 Permissible and impermissible loading of an angle rotor and a swing out rotor example illustrations NOTE Pay at...

Page 35: ...060 0020 0020 0050 Gef e 2 16KL bis 4 5L 27 m od_1405319211622_68 docx 192577 4 1 6 2 2 6 Vessels Load the vessels outside of the centrifuge Liquids in the buckets or multiple carriers cause corrosio...

Page 36: ...5319250704_68 docx 192619 3 1 6 3 1 User interface The centrifuge is operated via three buttons with integrated light emitting diodes and one function knob The display is divided into several differen...

Page 37: ...centrifugation run can be started A quick stop can also be triggered during a normal deceleration e g in order to speed up the deceleration When a quick stop is triggered fast will be displayed in th...

Page 38: ...speed and RCF can be changed during the centrifugation Fig 11 Setting the speed value or the RCF value Pos 134 010 Universalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 135 100 Sigma 100 BA...

Page 39: ...bol disappears but the bar continues to flash Press the function knob in order to activate the desired setting The bar remains visible as long as the cursor is placed on the symbol Fig 13 The function...

Page 40: ...entrifugen Sigma Standardm odul e 060 Betrieb_Spi ncontr ol L S Spincontrol L Zent6 060 0030 0020 0070 Tem per atur nur KL 27 m od_1405319266022_68 docx 192773 4 1 6 3 2 7 Temperature The set value is...

Page 41: ...s the set temperature can be modified within the range below the actual temperature The Rapid Temp program will be stopped under the following conditions The set value is reached The program will stop...

Page 42: ...al different types of possible buckets the various combinations will be displayed one after the other Confirm the input The selected rotor or rotor bucket combination will be adopted Fig 18 Preselecti...

Page 43: ...erzeile 0 mod_1202116244500_0 docx 114 1 Pos 143 100 Sigma 100 BA Zentrifugen Sigma Standardm odul e 060 Betrieb_Spi ncontr ol L S Spincontrol L Zent6 060 0030 0020 0100 Autom atische D eckel ffnung 2...

Page 44: ...red setting The bar remains visible as long as the cursor is placed over the symbol Fig 21 The sound signal Buzzer is activated Pos 146 010 Universalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1...

Page 45: ...der quit the cycle display Fig 23 Cycle display for rotor 11650 and bucket 13421 Reaching the maximum number of cycles When the maximum number of cycles is reached for a rotor or bucket a correspondin...

Page 46: ...activated Proceed in the same manner in order to deactivate the permanent input lock Fig 25 Padlock symbol indicating an activated input lock Pos 152 010 Universalmodul e Leerzeile 0 mod_120211624450...

Page 47: ...are indicated by a flashing display Any storage locations that are already occupied will be overwritten during the saving process Save the program in the desired location The program is now saved NOT...

Page 48: ...dm odul e 060 Betrieb_Spi ncontr ol L S 060 0040 Ausschalten 27 mod_1405319212716_68 docx 192591 2 1 6 4 Switching the centrifuge off Open the centrifuge when it is not in use so moisture can evaporat...

Page 49: ...orrectly Fuses have tripped Reactivate temperature fuse see chapter 5 2 1 Type of connection Mains power switch off Switch mains power switch on Centrifuge cannot be started start key LED is not illum...

Page 50: ...d Switch off the mains power switch and disconnect the power cord from the socket Remove the plug see figure item 1 from the opening at the side panel e g with a screwdriver Fig 28 Position of the ope...

Page 51: ...s Allow to slow down Power off Allow to cool down Provide better ventilation only air cooled centrifuges Provide sufficient water throughput only water cooled centrifuges 46 49 Imbalance error only fo...

Page 52: ...ies malfunctions or spare part enquiries From Germany Contact Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode Germany Tel 49 0 55 22 50 07 44 44 E mail support lab sigma zentrifugen...

Page 53: ...e g by heat generators Pos 174 010 Universalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 175 100 Sigma 100 BA Zentrifugen Sigma Standardm odul e 080 War tung und Instandhaltung 080 0010 W ar...

Page 54: ...0 War tung und Instandhaltung 080 0010 0020 Zubeh r au er 8K 28 m od_1405320618247_68 docx 194845 3 1 8 1 2 Accessories CAUTION For the care of the accessories special safety measures must be consider...

Page 55: ...ds Chemical reactions as well as stress corrosion combination of oscillating pressure and chemical reaction can affect or destroy the metals Barely detectable cracks on the surface can expand and weak...

Page 56: ...ased load bearing bolts ensure a uniform swing out of the buckets and therefore the smooth operation of the centrifuge Load bearing bolts that are insufficiently greased may cause the centrifuge to st...

Page 57: ...e upper third of the rotor chamber with e g Vaseline Then let the rotor rotate for a few minutes at a moderate speed approx 2000 rpm The glass and metal particles will now collect at the greased part...

Page 58: ...s Max temp C Min time min Max time min Max cycles Aluminium buckets 134 138 3 5 Aluminium rotors 134 138 3 5 Glass tubes 134 138 3 40 Polyallomer polycarbonate rectangular carriers 115 118 30 40 Polya...

Page 59: ...is high level of stress high quality components were used during the production of the centrifuge Nevertheless wear cannot be excluded and it may not be visible from the outside Especially the rubber...

Page 60: ...obliged to certify the harmlessness of all incoming goods For this purpose we require a declaration of decontamination The form must be filled in completely and signed by authorised and specialised p...

Page 61: ...Please use the original packaging for the unit if at all possible If the product is dispatched to us in unsuitable packaging you will be charged the cost for returning it to you in new packaging The...

Page 62: ...ld waste You can return these centrifuges free of cost to Sigma Laborzentrifugen GmbH Ensure that the unit is decontaminated Fill in a declaration of decontamination see chapter 8 4 Return of defectiv...

Page 63: ...Type 2 16KL Connection requirements Electr connection Protection class IP code see name plate I 20 Power consumption kW Input fuse AT 0 72 6 0 at 220 240 V 50 60 Hz temperature fuses Performance data...

Page 64: ...allowable relative humidity of air 80 from 5 C up to 31 C with a linear decrease to 67 relative humidity of air at 35 C Maximum altitude 2 000 m above sea level Pos 211 010 Universalmodul e Leerzeile...

Page 65: ...downloaded from www sigma zentrifugen de Pos 217 010 Universalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 218 010 Universalmodul e Leerzeile 0 mod_1202116244500_0 docx 114 1 Pos 219 100 Sig...

Page 66: ...16KL 11 Appendix 66 82 Version 08 2012 Rev 1 24 of 28 04 2022 sb Translation of the original operating manual part no 0700702 Fig 32 Microhaematocrit rotor Pos 220 010 Universalmodul e Seitenwechsel 0...

Page 67: ...manual part no 0700702 Pos 221 100 Sigma 100 BA Zentrifugen Sigma Standardm odul e 110 Anhang 110 0020 Dr ehzahl Schwer efeld Diagr amm 28 m od_1405320705642_68 docx 195153 2 1 11 2 Speed gravitation...

Page 68: ...eption is curve 0 It decelerates brakeless spin out In general the runtime until the set speed is reached depends on the moment of inertia of the rotor Linear curves The slope of the fixed acceleratio...

Page 69: ...16KS 4 16KHS 6 16S 6 16HS 6 16KS 6 16KHS 9366 15 000 4 5KL 4 16S 4 16KS 4 16KHS 6 16S 6 16HS 6 16KS 6 16KHS 11805 10 years 8KS 8KBS 11806 10 years 8KS 8KBS 12082 7 years 1 14 1 14K 12083 7 years 1 14...

Page 70: ...0 82 Version 08 2012 Rev 1 24 of 28 04 2022 sb Translation of the original operating manual part no 0700702 Pos 227 100 Sigma 100 BA Zentrifugen Sigma Standardm odul e 110 Anhang 110 0050 Best ndigkei...

Page 71: ...4 4 4 1 4 1 Allyl alcohol C3H6O 96 1 3 3 2 2 2 2 4 1 1 1 Aluminium chloride AlCl3 saturated 1 3 2 4 1 1 1 1 4 Aluminium sulfate Al2 SO4 3 10 1 1 1 3 1 1 1 1 1 1 1 Ammonium chloride NH4 Cl aqueous 1 1...

Page 72: ...ne C2H8N2 100 1 1 3 1 1 3 4 1 1 1 Diesel fuel 100 1 1 3 1 1 1 3 1 1 1 Dimethyl formamide DMF C3D7NO 100 1 1 4 1 1 4 3 1 3 1 Dimethyl sulfoxide DMSO C2H6SO 100 1 2 4 1 1 4 4 1 1 Dimethylaniline C8H11N...

Page 73: ...3 1 2 1 Methyl benzene C7H8 100 3 1 4 1 3 4 4 4 1 4 1 Methyl ethyl ketone MEK C4H8O 100 1 1 4 1 1 4 4 4 1 4 1 Methylene chloride CH2Cl2 100 4 3 4 3 3 4 4 4 1 1 Mineral oil 100 1 1 1 1 1 1 1 1 1 1 Nit...

Page 74: ...1 1 1 1 1 2 4 Sodium hydroxide NaOH 50 1 1 4 1 1 1 1 1 2 4 Sodium sulfate Na2SO4 10 1 1 1 1 1 1 1 1 1 1 1 Spirits C2H6O 96 1 1 1 1 1 1 1 3 1 1 Styrene C8H8 100 4 1 4 1 3 4 4 1 4 1 Sulphuric acid H2SO4...

Page 75: ...28 04 2022 sb 75 82 Translation of the original operating manual part no 0700702 Pos 229 100 Sigma 110 BA Zentrifugen Sigma Proj ekte 2 16KL_2 16KH L 110 Anhang 110 0060 Konformit tserkl rung 2 16KL_K...

Page 76: ...Refrigerated Centrifuge Sigma 2 16KL 11 Appendix 76 82 Version 08 2012 Rev 1 24 of 28 04 2022 sb Translation of the original operating manual part no 0700702...

Page 77: ...efrigerated Centrifuge Sigma 2 16KL 11 Appendix Version 08 2012 Rev 1 24 of 28 04 2022 sb 77 82 Translation of the original operating manual part no 0700702 11 7 Declaration of conformity China RoHS 2...

Page 78: ...Refrigerated Centrifuge Sigma 2 16KL 11 Appendix 78 82 Version 08 2012 Rev 1 24 of 28 04 2022 sb Translation of the original operating manual part no 0700702 Ende der Liste f r Textm arke Inhalt...

Page 79: ...trifuge is inclined 49 Centrifuge was moved during run 49 Centrifuge cleaning and care 53 Centrifuges definition 9 CE Zeichen gem Richtlinie 2006 42 EG 15 Changes in colour 58 Changes in structure 58...

Page 80: ...bearing bolts 53 56 H Hazard warnings 9 30 31 35 Heavy duty grease for load bearing bolts 56 Highly corrosive substances 21 I Imbalance 33 35 Imbalance error 51 Imbalance monitoring system 24 Importan...

Page 81: ...evention of accidents 9 Problem description 60 Process error 51 ProgLock program lock 44 Program Rapid Temp 41 Program lock ProgLock 44 Protection class 28 63 Q Qualified electrician 18 Quick stop 37...

Page 82: ...d accessories 57 Stopping of the centrifuge due to an imbalance 56 Storage and transport 26 Storage conditions 26 Storage locations 63 Stress corrosion see corrosion 55 Structural changes 21 Supply vo...

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