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Sigma 2-16KHL, Operating Manual

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Sigma 2-16KHL Operating Manual Download Page 12

Refrigerated Centrifuge with Heating System Sigma 2-16KHL 

  

 

2 Layout and mode of operation 

 

 

 

12 / 80 

Version 02/2013, Rev. 1.19 of 25/04/2019 

• sb 

Translation of the original operating manual, part no. 0700802 

 

 

2.1.2 

Name plate 

 

 

1

Manufacturer 

2

Power consumption 

3

Max. speed 

4

Max. kinetic energy 

5

Serial number 

6

Part number  

7

Type 

8

Nominal voltage 

9

CE mark in compliance 
with the directive  
2006/42/EC  

10

Symbol for special 
disposal (see chapter 
9 - "Disposal") 

11

Consult operating 
manual 

12  Date of manufacture 

13

Max. density 

14

Refrigerant data  

 

 

Fig. 3: Example of a name plate 

 

 

Summary of Contents for 2-16KHL

Page 1: ...he original operating manual sb 02 2013 Rev 1 19 of 25 04 2019 Sigma 2 16KHL part no 0700802 Sigma 2 16KHL from serial no 147260 Refrigerated Centrifuge with Heating System Operating Manual Please retain for later use ...

Page 2: ...Refrigerated Centrifuge with Heating System Sigma 2 16KHL 2 80 Version 02 2013 Rev 1 19 of 25 04 2019 sb Translation of the original operating manual part no 0700802 ...

Page 3: ... original operating manual part no 0700802 In case of inquiries please state the following numbers Order number Serial number Copyright by Sigma Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode am Harz Germany Tel 49 0 5522 5007 0 Fax 49 0 5522 5007 12 Web www sigma zentrifugen de E mail info sigma zentrifugen de ...

Page 4: ...Refrigerated Centrifuge with Heating System Sigma 2 16KHL 4 80 Version 02 2013 Rev 1 19 of 25 04 2019 sb Translation of the original operating manual part no 0700802 ...

Page 5: ...al force 14 2 2 2 2 Density 14 3 Safety 15 3 1 Marking of the unit 15 3 2 Explanation of the symbols and notes 16 3 3 Responsibility of the operator 17 3 4 Operating personnel 17 3 5 Informal safety instructions 17 3 6 Safety instructions 18 3 6 1 Electrical safety 18 3 6 2 Mechanical safety 18 3 6 3 Fire prevention 19 3 6 4 Chemical and biological safety 19 3 6 5 Safety instructions for centrifug...

Page 6: ...on of an angle rotor with a hermetic lid 29 6 2 2 3 Installation of the microhaematocrit rotor 30 6 2 2 4 Installation of accessories 31 6 2 2 5 Adapters 33 6 2 2 6 Vessels 33 6 3 Control system Spincontrol L 34 6 3 1 User interface 34 6 3 2 Manual mode 35 6 3 2 1 Starting a centrifugation run 35 6 3 2 2 Interrupting a centrifugation run 35 6 3 2 3 Interrupting a deceleration process 35 6 3 2 4 Se...

Page 7: ...uckets and carriers 53 8 1 4 Microhaematocrit rotor 53 8 1 5 Load bearing bolts 54 8 1 6 Glass breakage 55 8 2 Sterilisation and disinfection of the rotor chamber and accessories 55 8 2 1 Autoclaving 56 8 3 Service 57 8 4 Return of defective centrifuges or parts 58 9 Disposal 60 9 1 Disposal of the centrifuge 60 9 2 Disposal of the packaging 60 10 Technical data 61 10 1 Ambient conditions 62 10 2 ...

Page 8: ...Refrigerated Centrifuge with Heating System Sigma 2 16KHL Table of contents 8 80 Version 02 2013 Rev 1 19 of 25 04 2019 sb Translation of the original operating manual part no 0700802 ...

Page 9: ... 1 2 Intended use Centrifuges are power driven machines that separate liquids from solid matter liquid mixtures or solid mixtures by centrifugal force They are solely intended for this purpose Any other use beyond this area of application is regarded as improper use Sigma Laborzentrifugen GmbH cannot be held liable for any damage resulting from such improper use The intended use also includes obse...

Page 10: ...n that must not be duplicated distributed or communicated in any other way Non compliance may be prosecuted under criminal law 1 5 Standards and regulations EC declaration of conformity see chapter 11 6 EC declaration of conformity 1 6 Scope of supply The centrifuge comprises 1 power cord with an IEC C13 connector 1 rotor wrench Part no 930 100 1 tube 30 g heavy duty grease for load bearing bolts ...

Page 11: ...of the original operating manual part no 0700802 2 Layout and mode of operation 2 1 Layout of the centrifuge 2 1 1 Functional and operating elements 1 Lid 2 User interface see chapter 6 3 1 User interface 3 Mains switch Fig 1 Total view of the centrifuge 4 Name plate see chapter 2 1 2 Name plate 5 Mains power input Fig 2 Rear view of the centrifuge ...

Page 12: ...l part no 0700802 2 1 2 Name plate 1 Manufacturer 2 Power consumption 3 Max speed 4 Max kinetic energy 5 Serial number 6 Part number 7 Type 8 Nominal voltage 9 CE mark in compliance with the directive 2006 42 EC 10 Symbol for special disposal see chapter 9 Disposal 11 Consult operating manual 12 Date of manufacture 13 Max density 14 Refrigerant data Fig 3 Example of a name plate ...

Page 13: ... as a function of the angular velocity The bigger the radius in the rotor chamber is and the higher the speed is the higher the centripetal acceleration is However the forces acting on the rotor also increase Das 2 2 2 Area of application Depending on the area of application of the centrifuge and also on the particle size solids content and volume throughput of the mixture of substances that is to...

Page 14: ...stated formula If afterwards the speed or the radius is changed the resulting relative centrifugal force will be recalculated automatically by the control unit If the RCF is changed the speed will be adapted while the specified radius is maintained The speed gravitational field diagram provides an overview of the relationship between speed radius and RCF see chapter 11 2 Speed gravitational field ...

Page 15: ...ective earth ground CE mark in compliance with the directive 2006 42 EC Earth ground Do not dispose as part of domestic waste Unplug the mains plug GS mark tested safety only for Germany Arrow indicating the direction of rotation NRTL mark only for the USA and Canada Centrifuge with heating system RCM mark only for Australia Rotor loading information see chapter 6 2 2 4 Installation of accessories...

Page 16: ...rious health problems up to life endangering injuries DANGER This symbol stands for a direct hazard to the life and health of persons due to electrical voltage Non observance of these symbols causes serious health problems up to life endangering injuries WARNING This symbol stands for a potential hazard to the life and health of persons Non observance of these symbols can cause serious health prob...

Page 17: ... intended see chapter 1 2 Intended use take protective measures against fire and explosion when working with hazardous substances take measures for the safe opening of centrifuges 3 4 Operating personnel Persons operating the unit must be familiar with the fundamental regulations concerning workplace safety and accident prevention have read and understood this operating manual and in particular th...

Page 18: ...otion Do not reach into the rotor chamber when the rotor is in motion Do not use the centrifuge if it was installed incorrectly Do not use the centrifuge without panels Do not use the centrifuge if the rotors and inserts show signs of corrosion or other defects Only use the centrifuge with rotors and accessories that have been approved by the manufacturer In case of doubt contact the manufacturer ...

Page 19: ...or radioactive samples are intended to be used in the centrifuge it is in the responsibility of the user to ensure that all necessary safety regulations guidelines precautions and practices are adhered to accordingly DANGER Infectious toxic pathogenic and radioactive substances may only be used in special certified containment systems with a bio seal in order to prevent the material from being rel...

Page 20: ...ly warn against the use of equipment of poor quality Breaking glass or bursting vessels can cause dangerous imbalances at high speeds Ensure that rotor and buckets are correctly fitted see chapter 6 2 2 1 Installation of a rotor Observe the instructions on the installation of accessories see chapter 6 2 2 4 Installation of accessories WARNING The rotor must be loaded in a rotationally symmetrical ...

Page 21: ...ust be scrapped for reasons of safety If other data concerning the service life are engraved on the rotor or bucket these data shall apply accordingly For example a bucket with the engraving max cycles 10 000 has a service life of 10 000 cycles and a rotor with the engraving Exp date 02 20 must be scrapped in February 2020 at the latest see figure If a specification concerning the maximum number o...

Page 22: ...s with regard to plausibility The system continuously performs a self check and identifies malfunctions Error messages are displayed as Error followed by a code number see chapter 7 2 Table of error codes 3 7 4 Earth conductor check An earth conductor check can be carried out by authorised and specialised personnel using a suitable measuring instrument Please contact the Sigma service department s...

Page 23: ... 9 Remaining hazards The centrifuge was built in accordance with the state of the art and in compliance with the generally recognized safety rules However danger to life and limb of the operator or of third parties or impairments of the unit or other material assets cannot be completely excluded when the unit is being used Use the unit only for the purpose that it was originally intended for see c...

Page 24: ...he centrifuge only in dry rooms The permissible storage temperature is between 20 C and 60 C If you would like to store it for more than one year or if you intend to ship it overseas please contact the manufacturer 4 3 Notes on transport Install the transport safety device see chapter 4 5 Transport safety device Always lift the centrifuge with a lifting device or with a sufficient number of people...

Page 25: ...each under the centrifuge from the side CAUTION The centrifuge weighs approx 60 kg Retain the packaging for any possible future transport of the centrifuge 4 5 Transport safety device The transport safety device consists of a foamed plastic piece in the rotor chamber CAUTION The transport safety device must be removed prior to start up Removal Open the lid by pressing the lid key If the centrifuge...

Page 26: ... have a solid even surface Attention During transport from cold to warmer places condensational water will collect inside the centrifuge It is important to allow sufficient time for drying min 24 h before the centrifuge can be used again 5 2 Power supply 5 2 1 Type of connection DANGER The operating voltage on the name plate must correspond to the local supply voltage Sigma centrifuges are units o...

Page 27: ... up and connection Version 02 2013 Rev 1 19 of 25 04 2019 sb 27 80 Translation of the original operating manual part no 0700802 5 2 2 Customer provided fuses Typically the centrifuge must be protected with 16 Amp L or B fuses that are to be provided by the customer ...

Page 28: ...tarted if the lid is opened To close press with both hands slightly on the lid until the lock is audibly locked WARNING Do not place your fingers between the lid and the housing when closing the lid Risk of crushing 6 2 2 Installation of rotors and accessories 6 2 2 1 Installation of a rotor Open the centrifuge lid by pressing the lid key Unscrew the rotor tie down screw from the motor shaft count...

Page 29: ... 2 2 Installation of an angle rotor with a hermetic lid Open the centrifuge lid by pressing the lid key Unscrew the rotor tie down screw from the motor shaft counter clockwise Screw the rotor lid onto the rotor and tighten it by turning it clockwise Lower the rotor with the lid with its central bore straight down onto the motor shaft Insert the rotor tie down screw into the motor shaft Tighten the...

Page 30: ...r tie down screw with the rotor tie down screw of the microhaematocrit rotor part no 81543 included in the scope of supply of the microhaematocrit rotor and tighten it loosely Lower the rotor with its central bore straight down onto the motor shaft Tighten the rotor tie down screw clockwise with 3 Nm using the supplied rotor wrench In doing so hold the microhaematocrit rotor with one hand and tilt...

Page 31: ...or Turn the reader and perform a fine adjustment with the aid of the central eccentric mechanism in order to localize the O point and the maximum liquid point in the capillary tubes You can now read the percentage value Remove the capillary tubes Some of the tubes can also be evaluated with the reader outside of the rotor see the instructions for use on the back of the reader 6 2 2 4 Installation ...

Page 32: ...t the buckets and their suspensions are loaded evenly It is not permissible to load angle rotors on only one axis correct incorrect Fig 7 Permissible and impermissible loading of an angle rotor and a swing out rotor example illustrations NOTE Pay attention to the marking of the centrifuge see the illustration below Safety indications on the centrifuge must be kept readable at all times If necessar...

Page 33: ...essels outside of the centrifuge Liquids in the buckets or multiple carriers cause corrosion Fill the vessels carefully and arrange them according to their weight Imbalances result in the excessive wear of the bearings Always fill the tubes up to their useful volume the volume that is stated for the tube Follow the safety instructions and hazard warnings see chapter 3 Safety Maximum speed for tube...

Page 34: ...nd one function knob The display is divided into several different fields The various functions of the system can be called up by pressing and turning the function knob 1 Start key 2 Function knob 3 Display 4 Stop key 5 Lid key Fig 9 User interface of the Spincontrol L control system Display The centrifuge display has the following display fields 1 Speed RCF field 2 Several display fields e g for ...

Page 35: ...ntrifugation run can be started A quick stop can also be triggered during a normal deceleration e g in order to speed up the deceleration When a quick stop is triggered fast will be displayed in the lower right hand corner of the display 6 3 2 3 Interrupting a deceleration process Press the start key during a deceleration process in order to interrupt it and to restart the centrifuge 6 3 2 4 Selec...

Page 36: ...epend on the rotor that is used The parameters speed and RCF can be changed during the centrifugation Fig 11 Setting the speed value or the RCF value 6 3 2 2 Runtime The set runtime is displayed in the upper section of this field with the remaining runtime shown below The runtime is counted down from the set value starting with the start of the centrifuge and ending with the start of the decelerat...

Page 37: ...case the symbol disappears but the bar continues to flash Press the function knob in order to activate the desired setting The bar remains visible as long as the cursor is placed on the symbol Fig 13 The function Runtime as of the set speed is activated Continuous run During the continuous run the runtime of the centrifuge is unlimited and must be stopped manually The centrifuge accelerates during...

Page 38: ... speed are restored and displayed Fig 15 Indication Short during a short run 6 3 2 3 Temperature Temp The set value is displayed in the upper area of the field and the actual sample temperature is displayed in the lower area Temperatures between 10 C and 60 C can be preselected Fig 16 Setting the temperature Temperature control Depending on the substances to be centrifuged it may make sense to per...

Page 39: ...only the set temperature can be modified The Rapid Temp program will be stopped under the following conditions The set value is reached The program will stop with a sound signal if this function is activated The stop button is pressed The program will be stopped prematurely A parameter is changed except for the temperature and rotors buckets In this case the program will be aborted A short run is ...

Page 40: ...various combinations will be displayed one after the other Confirm the input The selected rotor or rotor bucket combination will be adopted Fig 18 Preselection of a rotor or a rotor bucket combination Automatic rotor identification system If the centrifuge is equipped with an automatic rotor identification system the input mode will be activated automatically if the system detects a different roto...

Page 41: ...of a curve here preselection of an acceleration curve 6 3 2 6 Automatic lid opening function AutoOpen The automatic lid opening function must be activated so that the lid opens automatically at the end of the operation In order to activate the automatic lid opening function Select the Auto Open symbol with the cursor and confirm the selection The symbol and the bar under the symbol start to flash ...

Page 42: ...ut the bar continues to flash Press the function knob in order to activate the desired setting The bar remains visible as long as the cursor is placed over the symbol Fig 21 The sound signal Buzzer is activated 6 3 2 8 Program lock ProgLock When the program lock is active it is impossible to save any new programs In this case the function save program is disabled In order to activate the program l...

Page 43: ...1650 and bucket 13421 Reaching the maximum number of cycles When the maximum number of cycles is reached for a rotor or bucket a corresponding warning signal will be displayed every time that the centrifuge is started the start key lid key and entire display will flash Fig 24 Display when the maximum number of cycles is reached flashing When the start key is pressed CYCLES will be displayed The ce...

Page 44: ...s when pressing it for the third time After the activation of this function the padlock symbol flashes The input lock is activated Proceed in the same manner in order to deactivate the permanent input lock Fig 25 Padlock symbol indicating an activated input lock 6 3 3 Program mode A program contains all the data that are required for a centrifuge run Certain sedimentation results can be repeated u...

Page 45: ...t a random storage location from the program selection list Free storage locations are indicated by a flashing display Any storage locations that are already occupied will be overwritten during the saving process Save the program in the desired location The program is now saved NOTE The functions Auto Open Buzzer ProgLock and Cycles cannot be entered as part of a program Fig 26 Saving a program 6 ...

Page 46: ...ifuge 46 80 Version 02 2013 Rev 1 19 of 25 04 2019 sb Translation of the original operating manual part no 0700802 6 4 Switching the centrifuge off Open the centrifuge when it is not in use so moisture can evaporate Switch the centrifuge off by pressing the mains power switch ...

Page 47: ... mains power switch on Centrifuge cannot be started start key LED is not illuminated Several possible causes Power off on If the error occurs again contact service Centrifuge cannot be started lid key LED flashes The lid lock is not closed correctly Open and close the lid If the error occurs again contact service Centrifuge decelerates during operation Brief mains power failure Press start key in ...

Page 48: ...sconnect the power cord from the socket Remove the plug see figure item 1 from the opening at the side panel e g with a screwdriver Fig 28 Position of the opening for the emergency lid release The plug is connected with a string Pull the string in order to release the lid Then reinsert the stopper into the opening WARNING Do not unlock or open the lid unless the rotor is at a standstill If the lid...

Page 49: ... to cool down Provide better ventilation only air cooled centrifuges Provide sufficient water throughput only water cooled centrifuges 46 49 Imbalance error only for centrifuges with imbalance monitoring system Allow to slow down Power off Eliminate the imbalance 50 59 Lid error Press lid key Close lid Remove foreign matter from the opening of the lid lock device With error 50 and 51 the centrifug...

Page 50: ... of queries malfunctions or spare part enquiries From Germany Contact Sigma Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode Germany Tel 49 0 55 22 50 07 44 44 E mail support lab sigma zentrifugen de Outside Germany Contact our agency in your country All agencies are listed at www sigma zentrifugen de Sales Partners NOTE If you would like to utilise our service please state the type of ...

Page 51: ...ive substances Do not use solvents Do not use agents with abrasive particles Do not expose the centrifuge and rotors to intensive UV radiation or thermal stress e g by heat generators 8 1 Maintenance 8 1 1 Centrifuge Unplug the mains power plug before cleaning Carefully remove all liquids including water and particularly all the solvents acids and alkaline solutions from the rotor chamber using a ...

Page 52: ...cessories special safety measures must be considered as these are measures that will ensure operational safety at the same time Immediately rinse off the rotor buckets or accessories under running water if they have come into contact with any liquids that may cause corrosion Use a brush for test tubes in order to clean the bores of angle rotors Turn the rotor upside down and allow it to dry comple...

Page 53: ...ress corrosion combination of oscillating pressure and chemical reaction can affect or destroy the metals Barely detectable cracks on the surface can expand and weaken the material without any visible signs Check the material regularly at least once a month for cracks visible damage of the surface pressure marks signs of corrosion other changes Check the bores of the rotors and multiple carriers R...

Page 54: ...ng out of the buckets and therefore the smooth operation of the centrifuge Load bearing bolts that are insufficiently greased may cause the centrifuge to stop due to an imbalance Clean the load bearing bolts and bucket groove in order to remove the old grease Apply a small amount of heavy duty grease for load bearing bolts ref no 71401 see the following picture to both load bearing bolts of a buck...

Page 55: ...r chamber In order to completely remove the glass particles and metal dust from the rotor chamber Grease the upper third of the rotor chamber with e g Vaseline Then let the rotor rotate for a few minutes at a moderate speed approx 2000 rpm The glass and metal particles will now collect at the greased part Remove the grease with the glass and metal particles with a cloth If necessary repeat this pr...

Page 56: ...ime min Max cycles Aluminium buckets 134 138 3 5 Aluminium rotors 134 138 3 5 Glass tubes 134 138 3 40 Polyallomer polycarbonate rectangular carriers 115 118 30 40 Polyallomer polycarbonate round carriers 115 118 30 40 Polyamide buckets 115 118 30 40 10 Polycarbonate polyallomer lids for angle rotors 115 118 30 40 20 Polycarbonate tubes 115 118 30 40 20 Polyphenylsulfone PPSU caps for buckets 134 ...

Page 57: ... cannot be excluded and it may not be visible from the outside Especially the rubber parts that are among other things part of the motor suspension are subject to ageing This is why we recommend having the centrifuge checked by the manufacturer during an inspection once per year in the operating state and once every three years in the dismantled state Rubber parts should be replaced after three ye...

Page 58: ...ose we require a declaration of decontamination The form must be filled in completely and signed by authorised and specialised personnel only Affix the original form in a clearly visible manner to the outside of the packaging NOTE We will return the part unit if no declaration of decontamination is provided 2 Form for the return of defective parts This form is for the product related data They fac...

Page 59: ... part no 0700802 NOTE The defective part unit must be packaged in a transport safe manner Please use the original packaging for the unit if at all possible If the product is dispatched to us in unsuitable packaging you will be charged the cost for returning it to you in new packaging The forms can be downloaded online from www sigma zentrifugen de Service Overhaul and repair ...

Page 60: ...t it is not permissible to dispose of the unit among household waste You can return these centrifuges free of cost to Sigma Laborzentrifugen GmbH Ensure that the unit is decontaminated Fill in a declaration of decontamination see chapter 8 4 Return of defective centrifuges or parts Comply with any other applicable local rules and regulations 9 2 Disposal of the packaging Use the packaging to retur...

Page 61: ...ower consumption kW Max current consumption A Input fuse AT 1 01 0 95 4 5 at 220 240 V 50 60 Hz 6 0 at 220 240 V 50 60 Hz temperature fuses Performance data Max speed rpm Max capacity ml Max gravitational field x g Max kin energy Nm 15 300 400 21 913 9 962 Other parameters Time range Temperature range Storage locations 10 sec 99 h 59 min short run continuous run 10 C to 60 C 50 Physical data Heigh...

Page 62: ...minal voltage of 100V or 200V a tolerance of 10 5 applies For indoor use only Allowable ambient temperature 5 C to 35 C Max allowable relative humidity of air 80 from 5 C up to 31 C with a linear decrease to 67 relative humidity of air at 35 C Maximum altitude 2 000 m above sea level 10 2 Technical documentation For environmental reasons the comprehensive technical documentation of the centrifuge ...

Page 63: ...omplete list of accessories can be downloaded from www sigma zentrifugen de 11 1 1 Rotor radii The information in the accessories table concerning the radius refers to the values of the respective rotor as shown below The radius calculation is described in chapter 2 2 2 1 Speed radius and relative centrifugal force Fig 30 Minimum and maximum radius of an angle rotor Fig 31 Minimum and maximum radi...

Page 64: ...rigerated Centrifuge with Heating System Sigma 2 16KHL 11 Appendix 64 80 Version 02 2013 Rev 1 19 of 25 04 2019 sb Translation of the original operating manual part no 0700802 Fig 32 Microhaematocrit rotor ...

Page 65: ... Heating System Sigma 2 16KHL 11 Appendix Version 02 2013 Rev 1 19 of 25 04 2019 sb 65 80 Translation of the original operating manual part no 0700802 11 2 Speed gravitational field diagram Fig 33 Speed gravitational field diagram ...

Page 66: ...rakeless spin out In general the runtime until the set speed is reached depends on the moment of inertia of the rotor Linear curves The slope of the fixed acceleration curves defines the time that is required to accelerate the rotor by 1 000 rpm Curve 9 is a special case compared to the other curves The centrifuge accelerates with maximum power The runtime until the set speed is reached depends so...

Page 67: ... Edition 12082 7 years 1 14 1 14K 12083 7 years 1 14 1 14K 12084 7 years 1 14 1 14K 12085 7 years 1 14 1 14K 12092 5 years 20x 1 14 1 14K 12093 5 years 20x 1 14 1 14K 12094 5 years 20x 1 14 1 14K 12096 5 years 20x 1 14 1 14K 12097 5 years 20x 1 14 1 14K 12101 5 years 20x 1 15 1 15K 1 15P 1 15PK 12124 5 years 20x 1 15 1 15K 1 15P 1 15PK 12126 5 years 20x 1 15 1 15K 1 15P 1 15PK 12134 5 years 20x 1 ...

Page 68: ...00 1 1 4 3 3 4 4 4 1 4 1 Allyl alcohol C3H6O 96 1 3 3 2 2 2 2 4 1 1 1 Aluminium chloride AlCl3 saturated 1 3 2 4 1 1 1 1 4 Aluminium sulfate Al2 SO4 3 10 1 1 1 3 1 1 1 1 1 1 1 Ammonium chloride NH4 Cl aqueous 1 1 1 2 1 1 1 1 1 1 3 Ammonium hydroxide NH3 H2O 30 1 3 4 1 1 2 1 1 1 Aniline C6H7N 100 1 3 4 1 2 4 4 4 1 4 1 Anisole C7H8O 100 3 4 4 1 4 4 2 1 4 1 Antimony trichloride SbCl3 90 1 4 1 4 1 1 1...

Page 69: ...Diaminoethane C2H8N2 100 1 1 3 1 1 3 4 1 1 1 Diesel fuel 100 1 1 3 1 1 1 3 1 1 1 Dimethyl formamide DMF C3D7NO 100 1 1 4 1 1 4 3 1 3 1 Dimethyl sulfoxide DMSO C2H6SO 100 1 2 4 1 1 4 4 1 1 Dimethylaniline C8H11N 100 3 4 2 4 1 1 Dioxane C4H8O2 100 2 1 4 1 3 2 3 4 1 3 1 Dipropylene glycol mono methyl ether C4H10O 100 3 1 4 1 4 4 4 4 1 1 Ethyl acetate C4H8O2 100 1 1 4 1 1 4 4 4 1 4 1 Ethylene chloride...

Page 70: ... 2 4 1 1 3 1 3 1 2 1 Methyl benzene C7H8 100 3 1 4 1 3 4 4 4 1 4 1 Methyl ethyl ketone MEK C4H8O 100 1 1 4 1 1 4 4 4 1 4 1 Methylene chloride CH2Cl2 100 4 3 4 3 3 4 4 4 1 1 Mineral oil 100 1 1 1 1 1 1 1 1 1 1 Nitric acid HNO3 10 1 4 1 4 1 1 1 1 4 3 Nitric acid HNO3 100 4 4 4 4 4 4 1 4 1 Nitrobenzene C6H5NO2 100 3 4 4 3 2 4 4 4 1 4 1 Oleic acid C18H34O2 100 1 1 1 2 1 1 1 3 1 Oxalic acid C2H2O4 x 2H...

Page 71: ... 1 1 1 1 1 1 1 3 Sodium hydroxide NaOH 30 1 1 4 1 1 1 1 1 1 2 4 Sodium hydroxide NaOH 50 1 1 4 1 1 1 1 1 2 4 Sodium sulfate Na2SO4 10 1 1 1 1 1 1 1 1 1 1 1 Spirits C2H6O 96 1 1 1 1 1 1 1 3 1 1 Styrene C8H8 100 4 1 4 1 3 4 4 1 4 1 Sulphuric acid H2SO4 6 1 4 1 4 1 1 1 1 2 3 Sulphuric acid H2SO4 fuming 4 4 4 4 4 4 4 4 1 4 3 Tallow 100 1 1 1 1 1 1 1 1 1 1 Tetrahydrofuran THF C4H8O 100 3 1 4 1 3 4 4 4 ...

Page 72: ...Refrigerated Centrifuge with Heating System Sigma 2 16KHL 11 Appendix 72 80 Version 02 2013 Rev 1 19 of 25 04 2019 sb Translation of the original operating manual part no 0700802 ...

Page 73: ...gerated Centrifuge with Heating System Sigma 2 16KHL 11 Appendix Version 02 2013 Rev 1 19 of 25 04 2019 sb 73 80 Translation of the original operating manual part no 0700802 11 6 EC declaration of conformity ...

Page 74: ...Refrigerated Centrifuge with Heating System Sigma 2 16KHL 11 Appendix 74 80 Version 02 2013 Rev 1 19 of 25 04 2019 sb Translation of the original operating manual part no 0700802 ...

Page 75: ...ed Centrifuge with Heating System Sigma 2 16KHL 11 Appendix Version 02 2013 Rev 1 19 of 25 04 2019 sb 75 80 Translation of the original operating manual part no 0700802 11 7 Declaration of conformity China RoHS 2 ...

Page 76: ...Refrigerated Centrifuge with Heating System Sigma 2 16KHL 11 Appendix 76 80 Version 02 2013 Rev 1 19 of 25 04 2019 sb Translation of the original operating manual part no 0700802 ...

Page 77: ...annot be started 47 Centrifuge decelerates during operation 47 Centrifuge is inclined 47 Centrifuge was moved during run 47 Centrifuge cleaning and care 51 Centrifuges definition 9 Changes in colour 56 Changes in structure 56 Chemical and biological safety 19 Chemical resistance of plastic 53 China RoHS 2 Declaration of conformity 75 Cleaning agents 55 Cleaning the bores of angle rotors 52 Cleanin...

Page 78: ...load bearing bolts 51 54 H Hazard warnings 9 28 29 33 Heavy duty grease for load bearing bolts 54 Highly corrosive substances 18 I Imbalance 31 33 Imbalance error 49 Imbalance monitoring system 22 Importance of the operating manual 9 Important information 16 Improper loading 47 Infectious substances 55 Inflammable substances 19 Informal safety instructions 17 Initial start up 28 Input fuse 61 Inpu...

Page 79: ...ure marks 53 Prevention of accidents 9 Problem description 58 Process error 49 ProgLock program lock 42 Program Rapid Temp 39 Program lock ProgLock 42 Protection class 26 61 Q Quick stop 35 Quitting the modification mode 35 R Radioactive substances 19 51 Radius 14 Rapid Temp program 39 Reaching the maximum number of cycles 43 Refrigerant 61 Refrigerant data 61 Relative centrifugal force RCF 14 36 ...

Page 80: ...f the centrifuge due to an imbalance 54 Storage and transport 24 Storage conditions 24 Storage locations 61 Stress corrosion see corrosion 53 Structural changes 19 Supply voltage 26 Switching the centrifuge off 46 Switching the centrifuge on 28 System check 22 System error 47 49 T Table of error codes 49 Table of rotors and accessories with a different service life 21 67 Technical data 61 Technica...

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