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Sigma 162923, Operating Manual

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Sigma 162923 Operating Manual Download Page 64

Blood bank centrifuge Sigma 8KBS 

  

 

6 Using the centrifuge 

 

 

 

64 / 125 

Version 05/2021, Rev. 1.2 of 28/04/2022 

• sb 

Translation of the original operating manual, part no. 0703502 

 

→

 

6.3.4 

Options for data input and output 

•  Serial interface (see chapter 6.3.5 - "Connection of a separate 

computer") 

•  External signal active DC 24 V, 0.5 A max. (part no. 17701) 
•  Floating switch AC 250 V max., 6 A (part no. 17702) 
•  Connection of a barcode scanner via a barcode extension board (see 

chapter 6.3.2.10 - "Option: Barcode menu") 

 

 

 

6.3.5 

Connection of a separate computer 

The back of the centrifuge is equipped with a serial interface (see chapter 
2.1.1 - "Functional and operating elements") for connecting a computer. 
•  Plug the connecting cable into the intended port on the back of the 

centrifuge. 

The centrifuge can now be controlled via the computer.  

 

 

NOTE 

If the centrifuge is controlled via the computer, the display can only be 
used for display purposes. Only the stop key is active at all times and can 
be used to switch the centrifuge off in an emergency situation.  

 

 

 

 

 

 

→

 

6.4 

Switching the centrifuge off 

•  Open the centrifuge when it is not in use so moisture can evaporate. 
•  Switch the centrifuge off by pressing the mains power switch. 

 

 

 

 

Summary of Contents for 162923

Page 1: ...ranslation of the original operating manual sb 05 2021 Rev 1 2 of 28 04 2022 Sigma 8KBS part no 0703502 Sigma 8KBS from serial no 162923 Blood bank centrifuge Operating Manual Please retain for later use ...

Page 2: ...Blood bank centrifuge Sigma 8KBS 2 125 Version 05 2021 Rev 1 2 of 28 04 2022 sb Translation of the original operating manual part no 0703502 ...

Page 3: ...2 sb 3 125 Translation of the original operating manual part no 0703502 Copyright by Sigma Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode am Harz Germany Tel 49 0 5522 5007 0 Fax 49 0 5522 5007 12 Web www sigma zentrifugen de E mail info sigma zentrifugen de ...

Page 4: ...Blood bank centrifuge Sigma 8KBS 4 125 Version 05 2021 Rev 1 2 of 28 04 2022 sb Translation of the original operating manual part no 0703502 ...

Page 5: ...ive centrifugal force 17 2 2 2 2 Density 17 3 Safety 18 3 1 Marking of the unit 18 3 2 Explanation of the symbols and notes 19 3 3 Responsibility of the operator 20 3 4 Requirements concerning the personnel 21 3 5 Informal safety instructions 22 3 6 Safety instructions 23 3 6 1 Electrical safety 23 3 6 2 Mechanical safety 23 3 6 3 Fire prevention 24 3 6 4 Chemical and biological safety 24 3 6 5 Sa...

Page 6: ...apters 37 6 2 2 4 Vessels and blood bag systems 37 6 3 Control system Spincontrol S 40 6 3 1 User interface 40 6 3 2 Manual mode 41 6 3 2 1 Starting a centrifugation run 41 6 3 2 2 Interrupting a centrifugation run 41 6 3 2 3 Interrupting a deceleration process 41 6 3 2 4 Selection display and modification of data 41 6 3 2 5 Standard menu 42 6 3 2 6 Process library menu 48 6 3 2 7 Parameters menu ...

Page 7: ...s buckets and carriers 71 8 1 4 Load bearing bolts 72 8 1 5 Glass breakage 73 8 2 Sterilisation and disinfection of the rotor chamber and accessories 73 8 2 1 Autoclaving 74 8 3 Service 75 8 4 Return of defective centrifuges or parts 76 9 Disposal 78 9 1 Disposal of the centrifuge 78 9 2 Disposal of the packaging 78 10 Technical data 79 10 1 Ambient conditions 80 10 2 Technical documentation 80 11...

Page 8: ...Blood bank centrifuge Sigma 8KBS Table of contents 8 125 Version 05 2021 Rev 1 2 of 28 04 2022 sb Translation of the original operating manual part no 0703502 ...

Page 9: ...erating instructions Transport and installation instructions of the blood bank centrifuge Sigma 8KBS part no 07041 1 3 Intended use The laboratory centrifuge is suitable for the separation of constituents of different densities in mixtures with a maximum density of 1 2 g cm3 The laboratory centrifuge marked with MD is intended for the separation of blood components that will be used for transfusio...

Page 10: ... for the operator and their personnel It includes instructions and information that must not be duplicated distributed or communicated in any other way Non compliance may be prosecuted under criminal law 1 6 Standards and regulations These operating instructions have been created in accordance with the relevant European standards and regulations see chapter 11 7 EC declaration of conformity 1 7 Sc...

Page 11: ...1 hose connector for condensate drain installed 80415 1 tube 30 g heavy duty grease for load bearing bolts 71401 1 operating manual incl EC declaration of conformity see chapter 11 7 EC declaration of conformity 07035 Options Description Part no alternative serial communication interface for serial communication RS232 with option to connect a barcode scanner without barcode scanner replaces interf...

Page 12: ... 2021 Rev 1 2 of 28 04 2022 sb Translation of the original operating manual part no 0703502 2 Layout and mode of operation 2 1 Layout of the centrifuge 2 1 1 Functional and operating elements 1 Lid 2 User interface see chapter 6 3 1 User interface Fig 1 Total view of the centrifuge ...

Page 13: ...yout and mode of operation Version 05 2021 Rev 1 2 of 28 04 2022 sb 13 125 Translation of the original operating manual part no 0703502 3 Locks of the front door 4 Name plate see chapter 2 1 2 Name plate Fig 2 Right side of the centrifuge ...

Page 14: ...ginal operating manual part no 0703502 5 Mains power switch 6 Condensate drain Fig 3 Left side of the centrifuge 7 Mains cable 8 Option connector for barcode scanner 9 RS 232 interface 10 Option serial interface see chapter 6 3 4 Options for data input and output 11 Castor 12 Adjustable foot Fig 4 Rear view of an air cooled centrifuge ...

Page 15: ...ifuge 2 1 2 Name plate 1 Manufacturer 2 Power consumption 3 Max speed 4 Max kinetic energy 5 Serial number 6 Part number 7 Nominal voltage 8 Type 9 Symbol for special disposal see chapter 9 Disposal 10 CE mark in compliance with the directive 2006 42 EC 11 Marking as per Regulation EU 2017 745 on medical devices 12 Date of manufacture 13 Consult operating manual 14 Max permissible density 15 Refri...

Page 16: ...n of the angular velocity The bigger the radius in the rotor chamber is and the higher the speed is the higher the centripetal acceleration is However the forces acting on the rotor also increase Das 2 2 2 Area of application Depending on the area of application of the centrifuge and also on the particle size solids content and volume throughput of the mixture of substances that is to be centrifug...

Page 17: ...mula If afterwards the speed or the radius is changed the resulting relative centrifugal force will be recalculated automatically by the control unit If the RCF is changed the speed will be adapted while the specified radius is maintained The speed gravitational field diagram provides an overview of the relationship between speed radius and RCF see chapter 11 2 Speed gravitational field diagram 2 ...

Page 18: ...n General danger 4 C Garantie Note concerning the condensate drain Name plate see chapter 6 2 2 2 Installation of accessories CE mark in compliance with the directive 2006 42 EC Do not dispose as part of domestic waste Consult operating manual Medical product in accordance with the regulation EU 2017 745 NRTL mark only for the USA and Canada RCM mark only for Australia China RoHS 2 mark only for C...

Page 19: ...problems up to life endangering injuries DANGER This symbol stands for a direct hazard to the life and health of persons due to electrical voltage Non observance of these symbols causes serious health problems up to life endangering injuries WARNING This symbol stands for a potential hazard to the life and health of persons Non observance of these symbols can cause serious health problems up to li...

Page 20: ...ake measures in order to prevent danger to life or health during work ensure that the centrifuges are used properly and entirely as intended see chapter 1 3 Intended use take protective measures against fire and explosion when working with hazardous substances take measures for the safe opening of the centrifuges The operator must perform a risk assessment concerning potential accidents in connect...

Page 21: ...ger zone or work area This manual uses the following personnel qualifications for various areas of activity Qualified electrician Due to their special training knowledge experience and familiarity with the relevant standards and regulations qualified electricians are in the position to perform work on electrical systems and to autonomously identify and prevent possible hazards Qualified electricia...

Page 22: ...son c a serious risk to public health 3 5 Informal safety instructions This operating manual is a part of the product The operating manual must be kept at the location of use of the centrifuge Ensure that it is accessible at all times The operating manual must be handed over to any subsequent owner or operator of the centrifuge Any changes additions or updates received must be added to the operati...

Page 23: ...e removed Inspect the electrical equipment of the unit regularly Defects such as loose or burnt cables must be eliminated immediately Following the completion of any type of repair or service the qualified and specialised personnel must perform final inspection and testing in compliance with the relevant standards 3 6 2 Mechanical safety In order to ensure the safe operation of the centrifuge obse...

Page 24: ... the centrifuge when it is not in use so that moisture can evaporate 3 6 3 Fire prevention DANGER Do not spin explosive or inflammable substances Do not use the centrifuge within hazardous locations 3 6 4 Chemical and biological safety If pathogenic toxic or radioactive samples are intended to be used in the centrifuge it is in the responsibility of the user to ensure that all necessary safety reg...

Page 25: ...gainst the use of equipment of poor quality Breaking glass or bursting vessels can cause dangerous imbalances at high speeds Ensure that rotor and buckets are correctly fitted see chapter 6 2 2 1 Installation of a rotor Observe the instructions on the installation of accessories see chapter 6 2 2 2 Installation of accessories The rotor must be loaded in a rotationally symmetrical manner at equal w...

Page 26: ... at least once per month for safety reasons Pay special attention to changes such as corrosion cracks material abrasion etc The rotors and accessories must be put out of service after 10 years Any use after this period may be permissible in individual cases after an inspection performed by the manufacturer After 50 000 cycles the rotor must be scrapped for reasons of safety If other data concernin...

Page 27: ... people helping you 3 6 8 2 Marking During production every rotor and bucket receives a batch number enabling conclusions to be drawn concerning the production process and the subsequent quality inspection Some rotors also have an additional serial number providing further detailed information The batch number and serial number is engraved on the rotor as follows 1 Batch number 2 Serial number Fig...

Page 28: ...signals with regard to plausibility Errors are detected with extreme sensitivity and displayed as error messages in a dialog box see chapter 7 2 Table of error codes 3 7 4 Earth conductor check An earth conductor check can be carried out by authorised and specialised personnel using a suitable measuring instrument Please contact the Sigma service department see chapter 7 3 Service contact 3 7 5 Im...

Page 29: ... hazards The centrifuge was built in accordance with the state of the art and in compliance with the generally recognized safety rules However danger to life and limb of the operator or of third parties or impairments of the unit or other material assets cannot be completely excluded when the unit is being used Use the unit only for the purpose that it was originally intended for see chapter 1 3 I...

Page 30: ...ge period of more than one year the specified maintenance intervals must be observed Any necessary work must be performed directly before using the device Specific dates of expiry of the accessories e g the limited usability of plastic rotors must be complied with These components must be replaced without exception 4 2 Transport The centrifuge must be transported by authorised specialised personne...

Page 31: ...med by authorised specialised personnel 5 1 Condensate drain The condensate drain is used to drain the condensate off that forms in the rotor chamber during the centrifugation It consists of a hose with a plastic valve It consists of a hose with a plastic valve and runs from the rotor chamber to the outlet on the left side in the front door of the centrifuge see chapter 2 1 1 Functional and operat...

Page 32: ...entrifuge The required accessories are included in the scope of supply 1 Holder for the rotor cover 2 Hexagon socket head screw M6x16 Fig 9 Cover holder accessories Installation There is a hole for the cover holder in the middle of the upper edge on the right hand side of the centrifuge Insert the hexagon socket head screw through the hole of the cover holder and fasten the cover holder to the hol...

Page 33: ...ion 6 2 Switching the centrifuge on Press the mains power switch The display then illuminates The centrifuge is ready for operation 6 2 1 Opening and closing the lid The lid can be opened if the centrifuge is at a standstill and if the lid key is illuminated Press the lid key in order to open the lid The centrifuge cannot be started if the lid is opened To close press with both hands slightly on t...

Page 34: ...ition the rotor carefully perpendicularly from above on the cone of the motor shaft see the illustration above item 2 CAUTION If the rotor gets jammed or if it is lowered too quickly onto the fastening stud the thread of the stud may be damaged In this case it is no longer possible to correctly secure the rotor in place Insert the supplied hexagon key part no 930 050 into the hexagon socket of the...

Page 35: ...rotor in place with one hand see the following picture and tighten it with the aid of the rotor wrench and a tightening torque of 20 Nm Fig 13 Fastening of the rotor WARNING After frequent use the rotor tie down screw must be loosened by some turns the rotor has to be lifted and fastened again This must be done once a day or after 20 cycles This ensures a proper connection between the rotor and th...

Page 36: ...g out rotors all of the compartments must be equipped with buckets Always load rotationally symmetrical compartments of the rotors with the same accessories and fill to avoid imbalance Centrifugation with vessels of various sizes Working with vessel of various sizes is possible In this case however it is very important that the rotationally symmetrical inserts are identical correct incorrect Fig 1...

Page 37: ... compartments of a carrier are not used the buckets must be loaded evenly Loading the edges of a bucket only is not permissible 6 2 2 4 Vessels and blood bag systems NOTE The information and instructions provided by the manufacturers of bags bottles and tubes for use in the centrifuge and especially the information concerning the maximum permissible RCF value and temperature must be observed Vesse...

Page 38: ...in particular for 250 and 500 ml bottles Blood bag systems Alle All six places on the rotor must be loaded with buckets It is required to fill just two opposite buckets must be filled with one adapter for blood bags and two blood bag systems each The opposite buckets including the filled blood bag systems must have an equal weight If the number of blood bags is uneven a balance adapter must be use...

Page 39: ...g von Blutbeutelsystemen WARNING The service life of the adapter for blood bags no 13867 is limited Refer to the table of rotors and accessories with a different service life see chapter 11 4 Table of the service life of rotors and accessories NOTE At maximum speed the plastic adapter 13867 must not be used at a temperature above 25 C If it is used at a considerably lower speed up to 2 500 rpm the...

Page 40: ...isplay is divided into several different fields The various functions of the system can be called up by pressing and turning the function knob 1 Start button 2 Display 3 Function knob 4 Stop button 5 Lid button Fig 18 User interface of the Spincontrol S control system Display The centrifuge display has the following fields 1 Menu bar 2 Speed field 3 RCF field 4 Rotor field 5 Program field 6 Status...

Page 41: ...efore a new centrifugation run can be started A quick stop can also be triggered during a normal deceleration e g in order to speed up the deceleration When a quick stop is triggered Quick stop will be displayed in the speed field NOTE A quick stop can be performed even if the centrifuge is blocked against unauthorised use 6 3 2 3 Interrupting a deceleration process Press the start key during a de...

Page 42: ...peed radius and relative centrifugal force The maximum speed values depend on the rotor that is used Relative centrifugal force RCF The relative centrifugal force is the acceleration that the sample is subjected to during the centrifugation run The set value of this parameter is displayed in the upper section of this field with the actual value shown below The values are stated in g gravitational ...

Page 43: ...ched Select the field Runtime and confirm the selection The display flashes when it is activated Turn the function knob from the time 0 00 10 anti clockwise or from the time 99 59 59 clockwise Infinite will be displayed After the start of the centrifuge the elapsed time will be displayed Deactivate the continuous run by pressing the stop button or by entering a specific runtime Short run A short r...

Page 44: ... data Fig 21 Rotor selection list here with potential rotor bucket combinations and additional information Automatic rotor identification The centrifuge automatically identifies the rotor that is currently being used If the system identifies a different rotor than the one that is set and if there are no different buckets for this rotor the rotor input will be adapted automatically The system will ...

Page 45: ...nu shows the program that is currently loaded When the field is selected the program list is displayed for information on how to work with the programs please see chapter 6 3 3 Program mode The program RAPID_TEMP see below cannot be deleted Fig 22 Program list Program RAPID_TEMP Precooling or preheating at a standstill may distort the measurement results and cause the increased wear of the mechani...

Page 46: ...s case the RAPID_TEMP program stops with a sound signal and the standstill cooling system will be activated The stop button is pressed The RAPID_TEMP program will be stopped prematurely No message will be issued when the set temperature is reached A parameter is changed except for the temperature or another input is made In this case the RAPID_TEMP program will be aborted No message will be issued...

Page 47: ...played temperature will deviate from the actual sample temperature Progress indicator The progress indicator provides a quick overview of the remaining runtime of the running centrifugation run For this purpose a green progress bar and percentage value are displayed in the program field Fig 24 Progress indicator during a centrifugation run After the completion of the centrifugation run the progres...

Page 48: ... The order of the processes corresponds to their storage locations on the program list Empty storage locations will not be displayed If more than 11 programs have been stored the user can scroll through the list Fig 25 Process library menu Loading a process In the process library select the desired process by turning the function knob Press the function knob in order to confirm the selection The p...

Page 49: ...he standstill cooling function is activated the centrifuge starts to precool after it is switched on In the runtime field the symbol is displayed The lid must be closed CAUTION Unmoved air in the rotor chamber leads to an incorrect measuring and control behaviour and causes the compressor to freeze over At temperatures below 0 C aqueous liquids will freeze thereby preventing sedimentation Do not c...

Page 50: ...ring function enables the continuous monitoring of the speed and runtime parameters during the centrifugation Activate the centrifugation monitoring function by clicking NOTE If the function is activated during a centrifugation run the monitoring process will not be started until the start of the next centrifugation run The centrifugation monitoring function compares the speed values of the curren...

Page 51: ...rrent temperature is between the preset temperature in the Standard menu and the Delta T limit value If the temperature leaves this range during the centrifugation run an error message will be displayed and the centrifuge will be decelerated to a complete standstill NOTE The delta T temperature monitoring system remains inactive as long as the RAPID_TEMP program is active see chapter 6 3 2 5 Stand...

Page 52: ...ters function is selected the Save function will also be selected Select the button Activate code Enter a four digit code and confirm the entry The lock is now active The symbol will be displayed in the status line and the lock level will be indicated If changes are made after a function has been blocked the system will ask for the code prior to executing the change Fig 28 Parameters menu with an ...

Page 53: ... Setup Function Open lid after run The automatic lid opening function ensures that the lid opens when the rotor has stopped CAUTION When the lid is open the cooling is not active The sample may warm up Program rotation See chapter 6 3 3 5 Automatic program rotation Runtime as of set speed If this function is active the runtime will not be measured until the set speed is reached In the Standard men...

Page 54: ...be displayed in C Celsius or F Fahrenheit Language The control system can be used in various language versions If a language is selected by mistake it can be changed on any screen as follows Press and hold the stop button Turn the function knob one notch to the left and then one notch to the right Release the stop button The Language window will be displayed Select the desired language Speed RCF f...

Page 55: ...tters and characters can be entered when the cursor flashes in the text field Turn the function knob in order to select a character and press it to confirm the selection Then press the knob again in order to enter the next character Pressing the arrow button will delete the last character The maximum number of characters is 19 When the name is complete select the option Accept and confirm it Buzze...

Page 56: ...If the curve number is already used the stored curve will be displayed The input field Int is used to specify the interval number of the process Up to ten intervals can be entered for a curve Enter the interval time of the current interval into the input field Time While doing so certain restrictions must be taken into consideration see below Enter the desired acceleration under min 1 rpm or RCF W...

Page 57: ...final speed 100 min 1 rpm runtime 1 hour not possible since the necessary slope 0 03 min 1 rpm which means that it is beyond the defined range Example 2 Start speed 0 min 1 rpm final speed 15000 min 1 rpm runtime 10 sec not possible since the necessary slope is 1500 min 1 rpm which means that it is beyond the defined range 6 3 2 10 Option Barcode menu NOTE In order to connect a barcode scanner the...

Page 58: ...8 barcode with the data structure 001 donation identification number and there are fewer than 12 of these codes stored in the system It is an ISBT 128 barcode with the data structure 020 staff member identification number and there are not any barcodes with this data structure stored in the system If the barcode is accepted the data of the last centrifugation run will be automatically deleted and ...

Page 59: ...ation If barcodes have been stored they will also be output as follows Syntax data structure 001 Barcode x abc r n x serial number starting at 1 abc barcode Syntax data structure 020 Barcode Staff Member ID abc r n abc barcode The enquiry can be performed at any time before during and after the centrifugation run Depending on the time of the enquiry some of the results may still be unknown 6 3 2 1...

Page 60: ...ains all of the data that are required for a centrifuge run Certain sedimentation results can be repeated under identical conditions Programs can be loaded executed edited and deleted when the centrifuge is at a standstill A maximum of 60 programs can be stored under the numbers 1 60 The program RAPID_TEMP does not occupy any storage location and cannot be deleted It is used to bring the centrifug...

Page 61: ...a letter and press it to confirm the selection Then the next character can be selected Pressing the arrow button will delete the last character When the program name is complete select OK and confirm it The program will be saved and the Standard menu will be displayed Fig 34 Assignment of a program name prior to saving the program 6 3 3 2 Loading a program Select the option Progr from the Standard...

Page 62: ... desired program from the list and confirm the selection by pressing the function knob Press the start button Or Open the process library select the desired program and press the start button The program will be executed and the Standard menu will be displayed 6 3 3 4 Deleting a program Select the option Progr in the Standard menu and confirm the selection The program list will be displayed Select...

Page 63: ...ded while the program rotation function is active this program will be used as the start program for the rotation After the completion of the program the next program on the program list will be loaded automatically The rotation continues up to the next empty storage location and then restarts from the beginning see the following illustration Example 1 Loading of Test04 Rotation Test04 Test05 Test...

Page 64: ... Connection of a separate computer The back of the centrifuge is equipped with a serial interface see chapter 2 1 1 Functional and operating elements for connecting a computer Plug the connecting cable into the intended port on the back of the centrifuge The centrifuge can now be controlled via the computer NOTE If the centrifuge is controlled via the computer the display can only be used for disp...

Page 65: ...e cannot be started start key LED is not illuminated Several possible causes Power off on If the error occurs again contact service Centrifuge cannot be started lid key LED flashes The lid lock is not closed correctly Open and close the lid If the error occurs again contact service Centrifuge decelerates during operation Brief mains power failure Press start key in order to restart the centrifuge ...

Page 66: ...ion of the openings for the emergency lid release Insert the supplied tube wrench part no 930 110 horizontally into the hole The key will be guided through a funnel shaped tube to the shaft of the lid lock motor Fig 40 The emergency lid release key must be inserted horizontally Unlock the motorised lid locks as follows Turn the left lid lock anti clockwise Turn the right lid lock clockwise Then re...

Page 67: ... Provide better ventilation only air cooled centrifuges Provide sufficient water throughput only water cooled centrifuges 46 49 Imbalance error only for centrifuges with imbalance monitoring system Allow to slow down Power off Eliminate the imbalance 50 59 Lid error Press lid key Close lid Remove foreign matter from the opening of the lid lock device With error 50 and 51 the centrifuge will stop 6...

Page 68: ...alfunctions or spare part enquiries From Germany Contact Sigma Laborzentrifugen GmbH An der Unteren Söse 50 37520 Osterode Germany Tel 49 0 55 22 50 07 44 44 E mail support lab sigma zentrifugen de Outside Germany Contact our agency in your country All agencies are listed at www sigma zentrifugen de Sales Partners NOTE If you would like to utilise our service please state the type of your centrifu...

Page 69: ...es Do not use solvents Do not use agents with abrasive particles Do not expose the centrifuge and rotors to intensive UV radiation or thermal stress e g by heat generators 8 1 Maintenance 8 1 1 Centrifuge Unplug the mains power plug before cleaning Carefully remove all liquids including water and particularly all the solvents acids and alkaline solutions from the rotor chamber using a cloth in ord...

Page 70: ... operational safety at the same time WARNING All rotors applicable for this centrifuge weigh more than 18 kg Always lift the rotors with a lifting device or with a sufficient number of people helping you Immediately rinse off the rotor buckets or accessories under running water if they have come into contact with any liquids that may cause corrosion Use a brush for test tubes to clean the bores of...

Page 71: ... the permanent high stress from high gravitational fields Chemical reactions as well as stress corrosion combination of oscillating pressure and chemical reaction can affect or destroy the metals Barely detectable cracks on the surface can expand and weaken the material without any visible signs Check the material regularly at least once a month for cracks visible damage of the surface pressure ma...

Page 72: ... Load bearing bolts that are insufficiently greased may cause the centrifuge to stop due to an imbalance Clean the load bearing bolts and bucket groove in order to remove the old grease Apply a small amount of heavy duty grease for load bearing bolts ref no 71401 see the following picture to both load bearing bolts of a bucket Fig 41 Sufficient quantity of grease for one bolt Install the bucket an...

Page 73: ... order to completely remove the glass particles and metal dust from the rotor chamber Grease the upper third of the rotor chamber with e g Vaseline Then let the rotor rotate for a few minutes at a moderate speed approx 2000 rpm The glass and metal particles will now collect at the greased part Remove the grease with the glass and metal particles with a cloth If necessary repeat this procedure 8 2 ...

Page 74: ...ycles Aluminium buckets 134 138 3 5 Aluminium rotors 134 138 3 5 Glass tubes 134 138 3 40 Polyallomer polycarbonate rectangular carriers 115 118 30 40 Polyallomer polycarbonate round carriers 115 118 30 40 Polyamide buckets 115 118 30 40 10 Polycarbonate polyallomer lids for angle rotors 115 118 30 40 20 Polycarbonate tubes 115 118 30 40 20 Polyphenylsulfone PPSU caps for buckets 134 138 3 5 100 P...

Page 75: ...s were used during the production of the centrifuge Nevertheless wear cannot be excluded and it may not be visible from the outside Especially the rubber parts that are among other things part of the motor suspension are subject to ageing This is why we recommend having the centrifuge checked by the manufacturer during an inspection once per year in the operating state and once every three years i...

Page 76: ...e a declaration of decontamination The form must be filled in completely and signed by authorised and specialised personnel only Affix the original form in a clearly visible manner to the outside of the packaging NOTE We will return the part unit if no declaration of decontamination is provided 2 Form for the return of defective parts This form is for the product related data They facilitate the a...

Page 77: ...3502 NOTE The defective part unit must be packaged in a transport safe manner Please use the original packaging for the unit if at all possible If the product is dispatched to us in unsuitable packaging you will be charged the cost for returning it to you in new packaging The forms can be downloaded online from www sigma zentrifugen de Service Overhaul and repair ...

Page 78: ...permissible to dispose of the unit among household waste You can return these centrifuges free of cost to Sigma Laborzentrifugen GmbH Ensure that the unit is decontaminated Fill in a declaration of decontamination see chapter 8 4 Return of defective centrifuges or parts Comply with any other applicable local rules and regulations 9 2 Disposal of the packaging Use the packaging to return the centri...

Page 79: ...0 Hz 16 0 at 3 x 400 V 50 Hz water cooled 32 0 at 3 x 220 V 60 Hz Performance data Max speed rpm Max capacity ml Max gravitational field x g Max kinetic energy Nm 4 100 12 000 5 394 280 080 Other parameters Time range Temperature range Storage locations 10 sec to 99 h 59 min 59 sec short run continuous run 20 to 40 C 60 Physical data Height mm Height with open lid mm Width mm Depth mm Weight kg No...

Page 80: ...mperature For indoor use only Allowable ambient temperature 5 C to 35 C Max allowable relative humidity of air 80 from 5 C up to 31 C with a linear decrease to 67 relative humidity of air at 35 C Maximum altitude 2 000 m above sea level 10 2 Technical documentation For environmental reasons the comprehensive technical documentation of the centrifuge e g circuit diagrams and the safety data sheets ...

Page 81: ...essories The complete list of accessories can be downloaded from www sigma zentrifugen de 11 1 1 Rotor radii The information in the accessories table concerning the radius refers to the values of the respective rotor as shown below The radius calculation is described in chapter 2 2 2 1 Speed radius and relative centrifugal force Fig 42 Minimum and maximum radius of a swing out rotor ...

Page 82: ...ifuge Sigma 8KBS 11 Appendix 82 125 Version 05 2021 Rev 1 2 of 28 04 2022 sb Translation of the original operating manual part no 0703502 11 2 Speed gravitational field diagram Fig 43 Speed gravitational field diagram ...

Page 83: ...by 1 000 rpm Curve 9 is a special case compared to the other curves The centrifuge accelerates with maximum power The runtime until the set speed is reached depends solely on the moment of inertia of the rotor Linear curve no Slope 0 4 rpm sec 1 6 rpm sec 2 8 rpm sec 3 17 rpm sec 4 25 rpm sec 5 33 rpm sec 6 50 rpm sec 7 100 rpm sec 8 200 rpm sec 9 1 000 rpm sec Fig 44 Slope of linear curves Quadra...

Page 84: ...k centrifuge Sigma 8KBS 11 Appendix 84 125 Version 05 2021 Rev 1 2 of 28 04 2022 sb Translation of the original operating manual part no 0703502 Fig 46 Diagram of linear curves Fig 47 Diagram of quadratic curves ...

Page 85: ...S 6 16KHS 9366 15 000 4 5KL 4 16S 4 16KS 4 16KHS 6 16S 6 16HS 6 16KS 6 16KHS 11805 10 years 8KS 8KBS 11806 10 years 8KS 8KBS 12082 7 years 1 14 1 14K 12083 7 years 1 14 1 14K 12084 7 years 1 14 1 14K 12085 7 years 1 14 1 14K 12092 5 years 20x 1 14 1 14K 12093 5 years 20x 1 14 1 14K 12094 5 years 20x 1 14 1 14K 12096 5 years 20x 1 14 1 14K 12097 5 years 20x 1 14 1 14K 12134 5 years 20x 1 16 1 16K 1...

Page 86: ...Blood bank centrifuge Sigma 8KBS 11 Appendix 86 125 Version 05 2021 Rev 1 2 of 28 04 2022 sb Translation of the original operating manual part no 0703502 ...

Page 87: ...4 4 4 1 4 1 Allyl alcohol C3H6O 96 1 3 3 2 2 2 2 4 1 1 1 Aluminium chloride AlCl3 saturated 1 3 2 4 1 1 1 1 4 Aluminium sulfate Al2 SO4 3 10 1 1 1 3 1 1 1 1 1 1 1 Ammonium chloride NH4 Cl aqueous 1 1 1 2 1 1 1 1 1 1 3 Ammonium hydroxide NH3 H2O 30 1 3 4 1 1 2 1 1 1 Aniline C6H7N 100 1 3 4 1 2 4 4 4 1 4 1 Anisole C7H8O 100 3 4 4 1 4 4 2 1 4 1 Antimony trichloride SbCl3 90 1 4 1 4 1 1 1 4 Benzaldehy...

Page 88: ...e C2H8N2 100 1 1 3 1 1 3 4 1 1 1 Diesel fuel 100 1 1 3 1 1 1 3 1 1 1 Dimethyl formamide DMF C3D7NO 100 1 1 4 1 1 4 3 1 3 1 Dimethyl sulfoxide DMSO C2H6SO 100 1 2 4 1 1 4 4 1 1 Dimethylaniline C8H11N 100 3 4 2 4 1 1 Dioxane C4H8O2 100 2 1 4 1 3 2 3 4 1 3 1 Dipropylene glycol mono methyl ether C4H10O 100 3 1 4 1 4 4 4 4 1 1 Ethyl acetate C4H8O2 100 1 1 4 1 1 4 4 4 1 4 1 Ethylene chloride C2H4Cl2 100...

Page 89: ...3 1 2 1 Methyl benzene C7H8 100 3 1 4 1 3 4 4 4 1 4 1 Methyl ethyl ketone MEK C4H8O 100 1 1 4 1 1 4 4 4 1 4 1 Methylene chloride CH2Cl2 100 4 3 4 3 3 4 4 4 1 1 Mineral oil 100 1 1 1 1 1 1 1 1 1 1 Nitric acid HNO3 10 1 4 1 4 1 1 1 1 4 3 Nitric acid HNO3 100 4 4 4 4 4 4 1 4 1 Nitrobenzene C6H5NO2 100 3 4 4 3 2 4 4 4 1 4 1 Oleic acid C18H34O2 100 1 1 1 2 1 1 1 3 1 Oxalic acid C2H2O4 x 2H2O 100 1 3 1 ...

Page 90: ...1 3 Sodium hydroxide NaOH 30 1 1 4 1 1 1 1 1 1 2 4 Sodium hydroxide NaOH 50 1 1 4 1 1 1 1 1 2 4 Sodium sulfate Na2SO4 10 1 1 1 1 1 1 1 1 1 1 1 Spirits C2H6O 96 1 1 1 1 1 1 1 3 1 1 Styrene C8H8 100 4 1 4 1 3 4 4 1 4 1 Sulphuric acid H2SO4 6 1 4 1 4 1 1 1 1 2 3 Sulphuric acid H2SO4 fuming 4 4 4 4 4 4 4 4 1 4 3 Tallow 100 1 1 1 1 1 1 1 1 1 1 Tetrahydrofuran THF C4H8O 100 3 1 4 1 3 4 4 4 1 3 1 Tetrahy...

Page 91: ...Blood bank centrifuge Sigma 8KBS 11 Appendix Version 05 2021 Rev 1 2 of 28 04 2022 sb 91 125 Translation of the original operating manual part no 0703502 11 6 Serial Control Interface Specification ...

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Page 117: ...Blood bank centrifuge Sigma 8KBS 11 Appendix Version 05 2021 Rev 1 2 of 28 04 2022 sb 117 125 Translation of the original operating manual part no 0703502 11 7 EC declaration of conformity ...

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Page 121: ...9 Carrier 37 Carrier systems 37 Carriers cleaning and care 71 CE mark in compliance with the directive 2006 42 EC 18 Centrifugation monitoring 50 Centrifugation of blood bag systems 38 Centrifugation of infectious toxic radioactive or pathogenic substances 24 Centrifugation principle 16 Centrifugation with low capacity 36 Centrifugation with vessels of various sizes36 Centrifuge cannot be started ...

Page 122: ...nsate off 31 Drive problem 65 E Earth conductor check 28 EC declaration of conformity 11 117 EEPROM error 67 Einsatz von Blutbeutelsysteme 38 Electrical connection 79 Electrical safety 23 Emergency lid release 66 Error correction 65 Error message 65 Executing a program 62 Explanation of the symbols and notes 19 Explosive substances 24 External signal 55 F Fastening stud 34 Filling quantity refrige...

Page 123: ...peed for tubes 38 Measures in the event of hazards and accidents 29 Mechanical safety 23 Menu Barcode 57 Menu Curve 56 Menu Help 59 Menu Parameters 49 Menu Processlibrary 48 Menu Setup 53 Menu Standard 42 Mode of operation 16 Modification mode active 41 Motor error 67 Motor shaft 69 Multiple carrier 37 N Name plate 15 No indication on the display 65 No power in the mains supply 65 Noise level 79 N...

Page 124: ... of set speed 53 S Safety area 25 Safety devices 28 Safety distance 23 25 Safety instructions 9 37 Safety instructions for centrifugation 25 Safety chemical and biological 24 Safety electrical 23 Safety mechanical 23 Safety conscious work 20 Saving a program 61 Scope of supply 10 Selection display and modification of data41 Serial number 15 68 75 Service 75 Service contact 68 Service life 69 Servi...

Page 125: ...hnical documentation 80 Temperature 42 Temperature at water inlet 79 Temperature error 67 Temperature inside the rotor chamber 28 Temperature monitoring 51 Temperature monitoring system 28 Temperature range 79 Temperature unit 54 Temperature value not reached 65 Thermal stress 69 Time fine 54 Time range 79 Toxic substances 24 69 Tubes 37 38 Two finger principle rotor fastening 34 Type 15 79 Type o...

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