Showa Denko Shodex GPC HK-400 Series Operation Manual Download Page 1 | Manualshive

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Operation Manual Shodex GPC HK-400 series

Version 201210E

Operation Manual

Shodex™

GPC HK-400 series

Europe, Middle East, Africa, Russia
www.shodex.de

Sales Office:
Showa Denko Europe GmbH
Shodex Business
Konrad-Zuse-Platz 3
81829 Munich
Germany

Technical Support:

[email protected]
+49 (0)89 / 93 99 62 37

Quotes and Orders:

[email protected]
+49 (0)89 / 93 99 62 34

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Summary of Contents for Shodex GPC HK-400 Series

Page 1: ...l Shodex GPC HK 400 series Europe Middle East Africa Russia www shodex de Sales Office Showa Denko Europe GmbH Shodex Business Konrad Zuse Platz 3 81829 Munich Germany Technical Support info shodex de 49 0 89 93 99 62 37 Quotes and Orders order shodex de 49 0 89 93 99 62 34 ...

Page 2: ... are matching and correct 1 Introduction Thank you for purchasing the Shodex product Shodex GPC HK 400 series is an ultra rapid analysis type size exclusion chromatography used with organic solvents The column series is suitable for the separations of organic compounds and or oligomers and molecular weight distribution analysis of high molecular weight polymers The columns with different exclusion...

Page 3: ... 1 000 000 1 000 000 F6025050 GPC HK 405 10 000 2 500 000 4 000 000 F6025060 GPC HK 406 30 000 8 000 000 10 000 000 Reference value only Measured with polystyrene 4 Usable Conditions 4 1 System Settings Product Name Flow Rate mL min Maximum Pressure MPa Per Column Temperature C Recommended Maximum Recommended Maximum GPC HK 401 0 3 1 0 2 0 25 40 60 GPC HK 402 1 5 20 GPC HK 403 2 0 25 GPC HK 404L G...

Page 4: ...ilter the sample solution using disposable 0 45 μm filter to prevent the column from clogging or deteriorating 3 To prepare a sample with molecular weight larger than 1 000 000 first allow the sample to stand in the eluent of analysis for 1 day until it becomes fully swollen Next slowly agitate the sample solution to completely dissolve the sample Be careful as aggressive agitation can cut the pol...

Page 5: ...upper stream position When connecting HK 404L use multiple number of the same columns in series This is because HK 404L is prepared by using several different gels with different pore sizes designed to provide linear calibration curves over a set molecular weight range If a HK 404L was used together with columns with different pore sizes this changes the mixing ratio of the gels and consequently i...

Page 6: ... the chromatograms Filter the eluent with a 0 45 μm disposable filter to prevent the problems 3 When replacing the eluent with miscible solvents first use 1 1 mixture of the current solvent and the new solvent and then replace it with 100 of the new eluent e g When replacing chloroform to DMF first run 1 1 mixture of chloroform and DMF and then introduce 100 DMF 4 Check miscibility solubility of t...

Page 7: ...and clean analytical columns Make sure to clean the analytical columns after removing the guard filter When washing the column let the washing solution flow from the column outlet go directly into the waste container and not let the solution go through the detector Cleaning Method Insoluble components that block the column inlet may be removed by reversing the flow direction i e introducing the el...

Page 8: ...arising out of in connection with the Shodex Column whether or not such damage is allegedly based on breach of warranty negligence or otherwise 3 No warranty is made in any of the following cases 3 1 If the Shodex product is not used in accordance with the operating manual 3 2 If the Shodex product is remodelled by anyone other than person or firm designated by Showa Denko K K 3 3 If the Shodex pr...

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