Operation Manual Shodex RSpak KC-811 and KC-811 6E
Version 2020
5
10. Performance Test
The column performance can be checked by calculating the number of theoretical plates under the
following conditions:
1) Specimen
3% Acetic acid
2) Injection amount
5 µL
3) Mobile phase
0.1% Phosphoric acid aqueous solution
4) Flow rate
1 mL/min
5) Column temperature
40°C
6) Detector
High-sensitivity refractive index detector such as Shodex RI
7) Calculation formula
NTP = 5.54 x (Rt/W)
2
where NTP: Number of theoretical plate, Rt: Retention time, W: Peak half width
11. Attention
1) Do not remove the end fittings of the column under any circumstances.
2) Do not make a strong impact on the column: such as hitting or dropping on the floor.
3) Replace the solvent in the LC system with the eluent to be used before connecting the column.
4) Connect the column so that the flow direction corresponds to the arrow mark on the tag.
5) When the column is not used for a month or more, replace the in-column solvent with the shipping
solvent, remove it from the LC system, close each end with a stopper, and store it at controlled room
temperature.
6) Filtrate the sample with a disposable filter (0 .45 µm) to prevent deterioration by adsorbing insoluble
matters.
7) Contact Shodex website (www.shodex.de) or Shodex partners regarding product and analysis
applications.