Shodex OHpak SB-2000 Series Operation Manual Download Page 1

ver.211025E

 

SHOWA DENKO K.K. (https://www.shodex.com/)

 

Operation Manual 

Shodex

 OHpak

 SB-2000 series 

 

Please read this manual carefully to achieve the best and consistent column performance for a long time

 

 
 

Important Handling Instructions 

 

 Caution! 

 

 

· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column 

and understand their proper handling methods to prevent potential health hazards or death 
from occurring. 

 

· Please wear appropriate personal protective equipment such as lab goggles and gloves 

when handling organic solvents and acid and alkaline reagents. Avoid any direct physical 
contact to prevent chemical injuries.   

 

Before Using the Column 

(1) Please visually inspect the package and outside of the column for any damage.   

 

(2) Please check if product name and serial number written on the column package, column adhesive label, and     

enclosed Certificate of Analysis (CoA) are matching and correct.   

 

1. Introduction 

Thank you for purchasing the Shodex™ product. Shodex

 OHpak

 SB-2000 series is a size exclusion 

chromatography column used with aqueous solvents. The column series is suitable for the preparations of 
various water-soluble high molecular weight polymers, proteins, and/or oligomers, and molecular weight 
distribution analysis of high molecular weight polymers. The columns with different exclusion limits are available. 
Please select columns with target molecular weight ranges (see below) that meet the molecular weight range 
of your samples.   
 

2. Column Components

                 

 

 

 
 
 

 
 
 
 

 

3. Column Specifications 

Product 

Code 

Product Name 

Column Size (mm) 

Particle 

Size (

μ

m) 

Theoretical 

Plate Number

 

(Per Column) 

Exclusion Limit

*1

 

I.D. Length 

 

F6516011 

OHpak SB-2002 

20.0 300  15 

  9,000 

    1,000 

F6516012 

OHpak SB-2002.5

 

20.0 300  10 

 12,000  

   10,000 

F6516013 

OHpak SB-2003

 

20.0 300  10 

 12,000 

100,000 

F6516014 

OHpak SB-2004

 

20.0 300  18 

 12,000 

1,000,000 

F6516015 

OHpak SB-2005

 

20.0 300  20 

 12,000 

  (4,000,000)

*2

 

F6516016 

OHpak SB-2006

 

20.0 300  20 

 12,000 

(20,000,000)

*2

 

F6516017 

OHpak SB-2006M 

20.0 300  20 

 12,000 

(20,000,000)

*2

 

F6709555 

OHpak SB-G 8B

 

 

8.0  

50  18  (Guard 

Column) 

 

SB-806M HQ is a mixed-gel column, suitable for the analysis of wide molecular weight ranges.   

 

      *1

 Reference value only / Measured with pullulan 

*2

 Estimated value 

Summary of Contents for OHpak SB-2000 Series

Page 1: ...ries is a size exclusion chromatography column used with aqueous solvents The column series is suitable for the preparations of various water soluble high molecular weight polymers proteins and or oligomers and molecular weight distribution analysis of high molecular weight polymers The columns with different exclusion limits are available Please select columns with target molecular weight ranges ...

Page 2: ...d to the eluent However since surfactants tend to remain on the column solvent replacement after their use takes a longer time than replacement from general solvents The replacement time can be shorten by using 30 to 50 v v methanol Organic solvents cannot be used for SB 2002 Attention Use the column within above stated flow rate pressure and temperature ranges Using the column outside the given r...

Page 3: ...t for analysis to dissolve or dilute samples If this is difficult use a solvent which has a composition that is as close as possible to the eluent s composition but which fully dissolves or dilutes the sample 2 Filter the sample solution using disposable 0 45 μm filter to prevent the column from clogging or deteriorating 3 To prepare a sample with molecular weight larger than 1 000 000 first allow...

Page 4: ... Set the initial flow rate at less than 1 0 mL min and start the system If using the column at an elevated temperature keep a low flow rate until the temperature of the column reaches to the set temperature and then gradually increase the flow rate to the desired 4 Multiple number of columns can be connected in series When connecting multiple columns with each having different exclusion limits set...

Page 5: ...mn first remove the guard column and check the performance of the analytical column alone If the problem is solved most likely the cause is from the guard column In this case clean the guard column If the problem is not solved by removing the guard column clean both guard and analytical columns Make sure to clean the guard and the analytical columns separately When washing the column let the washi...

Page 6: ...late Number N 2 Asymmetry Factor Fas 10 Additional Warnings 1 Do not remove end fittings 2 Do not make a strong impact on the column Do not drop or hit the column on a hard surface Refer to the Shodex website https www shodex com for product details and their applications For additional assistance contact the distributor from whom you purchased the column or contact your regional Shodex support of...

Page 7: ...anyone other than person or firm designated by Showa Denko K K 3 3 If the Shodex product is disposed of 3 4 If the Shodex product is resold by the user without giving prior written notice to Showa Denko K K 3 5 If the performance of the Shodex product is not conform to the specification of the attached CERTIFICATE OF ANALYSIS due to any of the reasons below a Computer virus b Impurities contained ...

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