Shodex IEC SP-FT 4A Operation Manual Download Page 1

ver.211110E

 

SHOWA DENKO K.K. (https://www.shodex.com/)

 

Operation Manual 

Shodex

 IEC SP-FT 4A 

 

(Please read this manual carefully to achieve the best and consistent column performance for a long time) 

 

 

Important Handling Instructions 

 

 Caution! 

 

 

· Please consult the Safety Data Sheet (SDS) of reagents and solvents used with the column 

and understand their proper handling methods to prevent potential health hazards or death 
from occurring. 

 

· Please wear appropriate personal protective equipment such as lab goggles and gloves 

when handling organic solvents and acid and alkaline reagents. Avoid any direct physical 
contact to prevent chemical injuries.   

 

Before Using the Column 

(1) Please visually inspect the package and outside of the column for any damage.   

 

(2) Please check if product name and serial number written on the column package, column name tag, and     

enclosed Certificate of Analysis (CoA) are matching and correct.   

 

1. Introduction 

Thank you for purchasing the Shodex

 product. 

Shodex

 

IEC SP-FT 4A is a strong cation exchange 

column filled with non-porous hydrophilic polymer-based gel modified with sulfopropyl group. Its small 
hydrophobic adsorption makes the column suitable for analyzing various proteins, peptides, and nucleic 
acids. The polymer-based gel allows its use at a wide pH range condition. 
 

2. Column Components   

 

           

 

 

 
 
 
 
 
 
 
 
 
 
 
3. Column Specifications 

Product 

Code 

Product Name 

Column Size 

(mm) 

Particle 

Size 

(

μ

m) 

Theoretical Plate 

Number 

(Per Column) 

Ion Exchange 

Capacity       

(meq/g) 

I.D. Length 

F6113100 

IEC SP-FT 4A 

4.6 

10 

2.7 

 20,000 

0.2 

 

Base Material 

: Non-porous particles of polyhydroxymethacrylate modified with sulfopropyl group

 

Column Housing  : PEEK 
Screw Type 

: Internally-threaded type No.10-32 UNF 

Shipping Solvent : 20 mM MES

*

 Buffer (pH5.6) 

*2-(N-Morpholino)ethanesulfonic acid

 
 
 
 

Summary of Contents for IEC SP-FT 4A

Page 1: ...and serial number written on the column package column name tag and enclosed Certificate of Analysis CoA are matching and correct 1 Introduction Thank you for purchasing the Shodex product Shodex IEC SP FT 4A is a strong cation exchange column filled with non porous hydrophilic polymer based gel modified with sulfopropyl group Its small hydrophobic adsorption makes the column suitable for analyzin...

Page 2: ...lt When using highly corrosive salts such as sodium chloride wash out the salts at the end of analysis The metal parts of the devices may rust Column pressure is influenced by the eluent composition flow rate and column temperature When changing the eluent compositions adjust the flow rate and column temperature so that the column pressure remains below the usable maximum pressure 5 Eluent Prepara...

Page 3: ... 2 Column Installation 1 Connect the column to LC system by following the flow direction arrow indicated on the column name tag If guard column is used position the guard column in front before the inlet of the analytical column 2 Make sure to insert the tubing all the way to the end fitting and secure it with the male nut It is important that there is no extra space between the tubing and the col...

Page 4: ...usable maximum pressure 7 4 Column Cleaning Problems in peak shapes and elution time changes or elevated column pressure etc are often caused by insoluble or adsorbing components present in the eluent and reagents being deposited inside the column These problems may be resolved by cleaning the column Make sure to clean the guard and the analytical columns separately When washing the column let the...

Page 5: ...late Number N 2 Asymmetry Factor Fas 10 Additional Warnings 1 Do not remove end fittings 2 Do not make a strong impact on the column Do not drop or hit the column on a hard surface Refer to the Shodex website https www shodex com for product details and their applications For additional assistance contact the distributor from whom you purchased the column or contact your regional Shodex support of...

Page 6: ...anyone other than person or firm designated by Showa Denko K K 3 3 If the Shodex product is disposed of 3 4 If the Shodex product is resold by the user without giving prior written notice to Showa Denko K K 3 5 If the performance of the Shodex product is not conform to the specification of the attached CERTIFICATE OF ANALYSIS due to any of the reasons below a Computer virus b Impurities contained ...

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